A5033674891- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Pancreatic function and diabetes
- Ion Channels and Receptors
- Genetics and Neurodevelopmental Disorders
- Hippo pathway signaling and YAP/TAZ
- Invertebrate Immune Response Mechanisms
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
- Insect Resistance and Genetics
- SARS-CoV-2 and COVID-19 Research
- Genomics and Rare Diseases
- Genetic and Kidney Cyst Diseases
- Genetics, Aging, and Longevity in Model Organisms
- Sphingolipid Metabolism and Signaling
- PI3K/AKT/mTOR signaling in cancer
- Antioxidant Activity and Oxidative Stress
- Metabolism and Genetic Disorders
National Centre for Biological Sciences
2016-2025
Tata Institute of Fundamental Research
2012-2023
Institute for Stem Cell Biology and Regenerative Medicine
2020-2022
Shanghai Jiao Tong University
2022
Keio University
2022
Estación Experimental del Zaidín
2022
University of Exeter
2022
Sanford Burnham Prebys Medical Discovery Institute
2022
Western University
2022
Heidelberg University
2022
Phosphatidylinositol transfer proteins (PITPs) are versatile required for signal transduction and membrane traffic. The best characterized mammalian PITPs the Class I PITPs, PITPα (PITPNA) PITPβ (PITPNB), which single domain with a hydrophobic cavity that binds phosphatidylinositol (PI) or phosphatidylcholine molecule. In this study, we report lipid binding properties of an uncharacterized soluble PITP, protein, cytoplasmic 1 (PITPNC1) (alternative name, RdgBβ), II family. We show protein...
Many membrane receptors activate phospholipase C (PLC) during signalling, triggering changes in the levels of several plasma (PM) lipids including PtdIns, PtdOH and PtdIns(4,5)P2. It is widely believed that exchange between PM endoplasmic reticulum (ER) required to restore lipid homeostasis PLC yet mechanism remains unresolved. RDGB a multi-domain protein with PITP domain (RDGB-PITPd). We find vitro, RDGB-PITPd binds transfers both PtdIns. In Drosophila photoreceptors experience high rates...
Phosphatidic acid (PA) is postulated to have both structural and signaling functions during membrane dynamics in animal cells. In this study, we show that before a critical time period rhabdomere biogenesis Drosophila melanogaster photoreceptors, elevated levels of PA disrupt transport the apical domain. Lipidomic analysis shows effect associated with an increase abundance single, relatively minor molecular species PA. These defects are dependent on activation state Arf1. Transport via...
During development, Drosophila larvae undergo a dramatic increase in body mass wherein nutritional and developmental cues are transduced into growth through the activity of complex signaling pathways. Class I phosphoinositide 3-kinases have an established role this process. In study we identify phosphatidylinositol 5-phosphate 4-kinase (dPIP4K) as kinase that regulates during larval development. Loss-of-function mutants dPIP4K show reduced weight prolonged whereas overexpression results both...
Membrane contact sites (MCS) between the plasma membrane (PM) and endoplasmic reticulum (ER) regulate Ca2+ influx. However, mechanisms by which cells modulate ER-PM MCS density are not understood, role of Ca2+, if any, in regulating these is unknown. We report that Drosophila photoreceptors, regulated channels, TRP TRPL. Regulation mediated extended synaptotagmin (dEsyt), a protein localized to previously shown density. find Ca2+-binding activity dEsyt required for its function vivo....
There is emerging evidence that there are shared genetic, environmental and developmental risk factors in psychiatry, cut across traditional diagnostic boundaries. With this background, the Discovery biology of neuropsychiatric syndromes (DBNS) proposes to recruit patients from five different (schizophrenia, bipolar disorder, obsessive compulsive Alzheimer's dementia substance use disorders), identify those with multiple affected relatives, invite these families participate study. The will...
During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, mechanism by which illumination is coupled to rhabdomere remains unclear. We find that contain a light-dependent phospholipase D (PLD) activity. loss PLD resulted an enhanced reduction size, accumulation Rab7 positive, rhodopsin1-containing vesicles (RLVs) cell body reduced rhodopsin protein. These phenotypes were...
Highlights•Drosophila PIP4K mutant larvae have increased PIP3 levels in cells•Cells show enhanced sensitivity to insulin the absence of PIP4K•PIP4K regulates enzymes involved turnover at plasma membrane•Loss suppresses resistance phenotypesSummaryPhosphatidylinositol 3,4,5-trisphosphate (PIP3) generation membrane is a key event during activation receptor tyrosine kinases such as required for normal growth and metabolism. We report that Drosophila, phosphatidylinositol 5 phosphate 4-kinase...
Abstract Lithium is an effective, well-established treatment for bipolar disorder (BD). However, the mechanisms of its action, and reasons variations in clinical response, are unclear. We used neural precursor cells (NPCs) lymphoblastoid cell lines (LCLs), from BD patients characterized response to lithium (using “Alda scale” “NIMH Retrospective Life chart method”), interrogate cellular phenotypes related both disease response. NPCs two biologically who differed their were compared with...
There are limited global data on head-to-head comparisons of vaccine platforms assessing both humoral and cellular immune responses, stratified by pre-vaccination serostatus. The COVID-19 vaccination drive for the Indian population in age group 18–45 years began April 2021 when seropositivity rates general were rising due to delta wave pandemic during April–May 2021. Between June 30, 2021, Jan 28, 2022, we enrolled 691 participants across four clinical sites India. In this non-randomised...