A5063984041- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Neurobiology and Insect Physiology Research
- CRISPR and Genetic Engineering
- Cannabis and Cannabinoid Research
- Mitochondrial Function and Pathology
- Parkinson's Disease Mechanisms and Treatments
- dental development and anomalies
- Genetics, Bioinformatics, and Biomedical Research
- Receptor Mechanisms and Signaling
- Collagen: Extraction and Characterization
- Toxin Mechanisms and Immunotoxins
- Lipid metabolism and biosynthesis
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
- Hippo pathway signaling and YAP/TAZ
- Photoreceptor and optogenetics research
- Bone and Dental Protein Studies
Research Network (United States)
2023-2024
University of Pennsylvania
2023-2024
National Centre for Biological Sciences
2016-2023
Activation of PINK1 and Parkin in response to mitochondrial damage initiates a that includes phosphorylation RAB7A at Ser72. Rubicon is binding negative regulator autophagy. The structure the Rubicon:RAB7A complex suggests Ser72 would block binding. Indeed, vitro by TBK1 abrogates Pacer, positive autophagy, has an RH domain with basic triad predicted bind introduced phosphate. Consistent this, Pacer-RH binds phosho-RAB7A but not unphosphorylated RAB7A. In cells, depolarization reduces...
During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, mechanism by which illumination is coupled to rhabdomere remains unclear. We find that contain a light-dependent phospholipase D (PLD) activity. loss PLD resulted an enhanced reduction size, accumulation Rab7 positive, rhodopsin1-containing vesicles (RLVs) cell body reduced rhodopsin protein. These phenotypes were...
Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling implicated human disease mechanisms. Biochemical analyses studies cultured cells have identified a large number proteins can mediate signaling. However, the role such regulating processes vivo development metazoans remains to be understood. Here, we describe set CRISPR-based genome engineering tools allow manipulation each these with spatial temporal control during metazoan...
ABSTRACT Mutations in genes that regulate mitophagy, a key mitochondrial quality control pathway, are causative for neurological disorders including Parkinson’s. Here, we identify novel stress response pathway activated by damage regulates mitophagy neurons. We find increasing levels of triggers graded, concerted induces proteasomal degradation negative regulators autophagy. These include Myotubularin-related phosphatase 5 (MTMR5), MTMR2 and Rubicon. This ‘ Mito phagic S tress R esponse’...
Lipid transfer proteins (LTPs) that shuttle lipids at membrane contact sites (MCS) play an important role in maintaining cellular homeostasis. One such LTP is the Retinal Degeneration B (RDGB) protein. RDGB localized MCS formed between endoplasmic reticulum (ER) and apical plasma (PM) Drosophila photoreceptors where it transfers phosphatidylinositol (PI) during G-protein coupled phospholipase C signalling. Previously, C-terminal domains of have been shown to be essential for its function...
ABSTRACT During phospholipase C-β (PLC-β) signalling in Drosophila photoreceptors, the phosphatidylinositol transfer protein (PITP) RDGB, is required for lipid at endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (MCS). Depletion of RDGB or its mis-localization away from ER–PM MCS results multiple defects photoreceptor function. Previously, interaction between FFAT motif and integral ER dVAP-A was shown to be essential accurate localization MCS. Here, we report that FFAT/dVAP-A...
Collagen is a protein that found in cartilage, bone and other tissues animals humans. People utilize collagen from chicken for medicine. It used to treat joint pain associated with many types of arthritis surgery, back pain, neck subsequent injury. So far, 26 genetically distinct have been described. However, there not sufficient information know if safe or what the side effects might be. Other products, such as bovine gelatin, caused allergic reactions whereas has low amount reactions....
Summary Inter-organelle communication between closely apposed membranes is proposed at Membrane Contact Sites (MCS). However the regulation of MCS structure and their functional relevance in vivo remain debated. The extended synaptotagmins (Esyt) are evolutionarily conserved proteins to function MCS. However, loss all three Esyts yeast or mammals shows minimal phenotypes questioning importance Esyt. We report that Drosophila photoreceptors, number regulated by PLCβ activity. Photoreceptors a...
SUMMARY Activation of PINK1 and Parkin in response to mitochondrial damage initiates a cytoprotective mitophagy that includes phosphorylation RAB7A at Ser72. Rubicon is binding protein acts as negative regulator autophagy. The structure the Rubicon-RAB7A complex suggests Ser72 would block binding. Indeed, vitro by TBK1 abrogates Pacer, positive autophagy, has an RH domain with basic triad predicted bind introduced phosphate. Consistent this, Pacer-RH binds phosho-RAB7A but not...
Summary In Drosophila photoreceptors, following Phospholipase C-β activation, the phosphatidylinositol transfer protein (PITP) RDGB, is required to maintain lipid homeostasis at endoplasmic reticulum (ER) plasma membrane (PM) contact sites (MCS). Depletion or mis-localization of RDGB results in multiple defects photoreceptors. Previously, interaction between FFAT motif with integral ER dVAP-A was shown be important for its localization ER-PM MCS. Here, we report that addition motif, a large...
Summary Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signalling implicated human disease mechanisms. Biochemical analyses studies cultured cells have identified a large number proteins can mediate signalling. However, the role such regulating processes vivo development metazoans remains to be understood. Here we describe set CRISPR based genome engineering tools allow manipulation each these with spatial temporal control during...
Abstract During phospholipase C-β (PLC-β) signalling in Drosophila photoreceptors, the phosphatidylinositol transfer protein (PITP) RDGB, is required for lipid at endoplasmic reticulum (ER)-plasma membrane (PM) contact sites (MCS). Depletion of RDGB or its mis-localization away from ER-PM MCS results multiple defects photoreceptor function. Previously, interaction between FFAT motif and integral ER dVAP-A was shown to be essential accurate localization MCS. Here, we report that FFAT/dVAP-A...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Bishal Basak author on ‘Interdomain interactions regulate localization lipid transfer protein at ER-PM contact sites’, BiO. PhD student lab Professor Raghu Padinjat National Center for Biological Sciences, Rajiv Gandhi Nagar, Kodigehalli, Bengaluru, Karnataka, India, investigating non-vesicular...