A5054319229- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Retinal Development and Disorders
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Nanopore and Nanochannel Transport Studies
- Endoplasmic Reticulum Stress and Disease
- Neurobiology and Insect Physiology Research
- Protein Kinase Regulation and GTPase Signaling
- Photoreceptor and optogenetics research
- Reservoir Engineering and Simulation Methods
- Neuroscience and Neuropharmacology Research
- Advanced Proteomics Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Mitochondrial Function and Pathology
- Nanoparticle-Based Drug Delivery
- Force Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Polymer Surface Interaction Studies
- Seismology and Earthquake Studies
- Mesoporous Materials and Catalysis
Shell (Netherlands)
2025
Yale University
2022-2024
National Centre for Biological Sciences
2015-2022
Shell (India)
2022
Manipal Academy of Higher Education
2015-2018
Large-scale deployment of carbon capture and storage (CCS) technology is imperative for mitigating greenhouse gas emissions to combat the effects climate change. One key elements success CCS selection appropriate sites store captured CO2. To achieve this goal, various factors such as plume migration, capacity, containment need be validated over long-time horizons. Flow simulators, which accurately model complex interplay buoyancy, viscous, capillary forces, are typically used modeling...
Many membrane receptors activate phospholipase C (PLC) during signalling, triggering changes in the levels of several plasma (PM) lipids including PtdIns, PtdOH and PtdIns(4,5)P2. It is widely believed that exchange between PM endoplasmic reticulum (ER) required to restore lipid homeostasis PLC yet mechanism remains unresolved. RDGB a multi-domain protein with PITP domain (RDGB-PITPd). We find vitro, RDGB-PITPd binds transfers both PtdIns. In Drosophila photoreceptors experience high rates...
In recent years, medium-range AI weather forecasting models have improved significantly, now offering accuracy comparable to classical numerical prediction (NWP) models, while also being faster and (once trained) less computationally demanding. Due inherent assumptions limitations, all exhibit some degree of persistent systematic errors, called biases, in their forecast output, with certain performing better than others for specific variables regions. To address these we introduce a machine...
During illumination, the light-sensitive plasma membrane (rhabdomere) of Drosophila photoreceptors undergoes turnover with consequent changes in size and composition. However, mechanism by which illumination is coupled to rhabdomere remains unclear. We find that contain a light-dependent phospholipase D (PLD) activity. loss PLD resulted an enhanced reduction size, accumulation Rab7 positive, rhodopsin1-containing vesicles (RLVs) cell body reduced rhodopsin protein. These phenotypes were...
Native mass spectrometry (nMS) has emerged as a key analytical tool to study the organizational states of proteins and their complexes with both endogenous exogenous ligands. Specifically, for membrane proteins, it provides dimension determine identity bound lipids decipher effects on observed structural assembly. We recently developed an approach directly from intact tunable lipid membranes where biophysical properties its compositions can be customized. Extending this, we use our...
Here, we introduce the full functional reconstitution of genetically validated core protein machinery (SNAREs, Munc13, Munc18, Synaptotagmin, and Complexin) for synaptic vesicle priming release in a geometry that enables detailed characterization fate docked vesicles both before after is triggered with Ca 2+ . Using this setup, identify new roles diacylglycerol (DAG) regulating -triggered involving SNARE assembly chaperone Munc13. We find low concentrations DAG profoundly accelerate rate...
Abstract Phosphatidylserine externalization on the surface of dying cells is a key signal for their recognition and clearance by macrophages mediated members X-Kell related (Xkr) protein family. Defective Xkr-mediated scrambling impairs clearance, leading to inflammation. It was proposed that activation Xkr4 apoptotic scramblase requires caspase cleavage, followed dimerization ligand binding. Here, using combination biochemical approaches we show purified monomeric, full-length human (hXkr4)...
Here we introduce the full functional reconstitution of genetically-validated core protein machinery (SNAREs, Munc13, Munc18, Synaptotagmin, Complexin) for synaptic vesicle priming and release in a geometry that enables detailed characterization fate docked vesicles both before after is triggered with Ca
Phosphatidylcholine (PC)-specific phospholipase D (PLD) hydrolyzes the phosphodiester bond of PC to generate phosphatidic acid (PA) and regulates several subcellular functions. Mammalian genomes contain two genes encoding distinct isoforms PLD in contrast with invertebrate that include a single gene. However, significance within genome same biochemical activity remains unclear. Recently, loss function only gene Drosophila was reported result reduced PA levels PA-dependent collapse...
Abstract The intricate molecular environment of the native membrane profoundly influences every aspect protein (MP) biology. Despite this, most prevalent method studying MPs uses detergent-like molecules that disrupt and remove this vital local context. This severely impedes our ability to quantitatively decipher context comprehend its regulatory role in structure, function, biogenesis MPs. Using a library membrane-active polymers we have developed platform for high-throughput analysis...
Abstract The native membrane environment profoundly influences every aspect of protein (MP) biology. Despite this, the most prevalent method studying MPs uses detergents to disrupt and remove this vital context, impeding our ability decipher local molecular context its effect. Here we develop a proteome-wide platform that enables rapid spatially resolved extraction target directly from cellular membranes into nanodiscs maintain using library membrane-active polymers. We accompany with an...
ABSTRACT Hierarchical organization of integral membrane proteins (IMP) and lipids at the is essential for regulating myriad downstream signaling. A quantitative understanding these processes requires both detections oligomeric IMPs directly from intact membranes determination key components/properties that regulate them. Addressing this, we have developed a platform enables native mass spectrometry (nMS) analysis IMP-lipid complexes customizable lipid membranes. Both composition properties...