A5103792213- Sperm and Testicular Function
- Influenza Virus Research Studies
- Respiratory viral infections research
- Reproductive Biology and Fertility
- Lipid Membrane Structure and Behavior
- COVID-19 epidemiological studies
- Animal Genetics and Reproduction
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- Polyamine Metabolism and Applications
- Viral Infections and Outbreaks Research
- Advanced Biosensing Techniques and Applications
- Metabolism and Genetic Disorders
- Vaccine Coverage and Hesitancy
- Redox biology and oxidative stress
- Pain Mechanisms and Treatments
- Reproductive biology and impacts on aquatic species
- melanin and skin pigmentation
- Proteins in Food Systems
- Nanoparticle-Based Drug Delivery
- Data-Driven Disease Surveillance
- Clinical Nutrition and Gastroenterology
- Hormonal and reproductive studies
- Botulinum Toxin and Related Neurological Disorders
- Force Microscopy Techniques and Applications
York Teaching Hospital NHS Foundation Trust
2022-2025
Research Network (United States)
2024
Yale University
2022-2024
Siemens Healthcare (United States)
2024
Great Ormond Street Hospital
2023
University College London
2023
Chattanooga State Community College
2022
Memphis Health Center
2022
Prisma Health
2022
University of South Carolina
2022
The formulation of accurate clinical case definitions is an integral part effective process public health surveillance. Although such should, ideally, be based on a standardized and fixed collection defining criteria, they often require revision to reflect new knowledge the condition involved improvements in diagnostic testing. Optimal also need have balance sensitivity specificity that reflects their intended use. After 2009-2010 H1N1 influenza pandemic, World Health Organization (WHO)...
Influenza vaccination is recommended especially for persons at risk of complications. In 2003, the World Health Assembly urged Member States (MS) to increase coverage 75% among older by 2010.To assess progress towards 2010 goal and describe seasonal influenza recommendations in Organization (WHO) European Region.Data on vaccine recommendations, dose distribution, target group were obtained from two sources: Union Economic Area MS data extracted surveys covering seven seasons...
ABSTRACT The role in early development of proteins secreted by oviduct epithelial cells has been investigated. Secreted devoid serum contamination have produced the surgical removal and immediate incubation [3sS]methionine-containing medium. After electrophoretic separation, polypeptides could be divided into those that were uniformly throughout oestrous cycle a second class showed cyclical pattern secretion. first represented small proportion total output whilst predominant was composed...
1. Protein synthesis has been investigated in different regions of the rat epididymis by measuring incorporation [35S]methionine tissue minces incubated vitro followed analysis labelled proteins on polyacrylamide gels containing sodium dodecyl sulphate. Rates were highest proximal cauda > distal initial segment ductuli efferentes corpus caput caput. One protein (mol.wt. 23 000) characterized segment, three (mol.wts. 18 500, 19 000 and 32 one 47 cauda. 2. After castration, all was reduced to...
Although influenza-like illnesses (ILI) and acute respiratory (ARI) surveillance are well established in Europe, the comparability of intensity among countries seasons remains an unresolved challenge. The objective is to compare ILI ARI some European countries.Weekly incidence rates proportion primary care consultations were modeled 28 for 1996/1997-2013/2014 using moving epidemic method (MEM). We calculated threshold three thresholds, which delimit five levels: baseline, low, medium, high,...
Abstract Recent evidence indicates that a carbohydrate recognition mechanism is involved in the early stages of sperm-egg interaction mammals. In this communication, we describe potential zona-ligand molecule on boar spermatozoa has capacity to recognize and bind moieties zona pellucida glycoproteins as well neoglycoproteins, BSA-fucose BSA-mannose. The broad specificity for binding there requirement polysaccharide structure or ‘clustering’ saccharides protein backbone. been identified...
Glycoproteins on the plasma membrane of testicular and cauda epididymidal spermatozoa have been labeled with galactose oxidase/NaB [3H]4 sodium metaperiodate/NaB[3H]4, followed by analysis SDS polyacrylamide gels. The major glycoprotein labeling has a molecular weight 110,000 whereas greater than 90% radio-label is incorporated into proteins 32,000. These 32,000-mol wt X are homologous similar purified from epididymal secretion which shown previously to be synthesized in caput epididymidis...
We have developed a chemiluminescent immunoenzymometric system. The first commercial application of this assay (CLA) is the measurement total IgE and allergen-specific in human serum. CLA system second-generation adaptation MAST RIA allergy profiling protocol consists three steps: overnight incubation serum, 4-h with enzyme-labeled antibody, 30-min reaction, which produces visible image (immunograph) on high-speed Polaroid instant film. densities bands produced film are quantified an...
Our objective was to identify any changes that occur in the composition of porcine zona pellucida during development 2- 4-cell embryo from oocyte. Oocytes, unfertilized eggs and single embryos have been recovered surgically their zonae pellucidae 125I-labelled analysed individually by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The ovulated possessed two major glycoproteins Mr250 000 90 which were absent follicular oocytes but present fluid oestrus, not luteal,...
The major glycoprotein on the plasma membrane of testicular spermatozoa labelled with galactose oxidase/NaB3H4 technique has mol.wt. 110 000. As pass through epididymis, labelling this disappears and is replaced by a 32 000-mol.wt. protein. latter protein component epididymal secretions. evidence suggests that it inserted into or absorbed to membrane, since its appearance correlates acquisition fertilizing capacity should serve as good marker for assessing maturation in vitro.
<section class="abstract"> The trypsin-like proteinase (TLP) of rabbit spermatozoa (Stambaugh & Buckley, 1969) attracted interest following the discovery that trypsin inhibitors impede penetration through zona pellucida (Stambaugh, Brackett Mastroianni, 1969; Zaneveld, Robertson Williams, 1970). Stambaugh Buckley (1969) recovered most this in an acrosomal fraction prepared by treating with surfactant Hyamine 2389 (Hartree Srivastava, 1965). Using fluorescein-labelled inhibitors, they...
Background AbobotulinumtoxinA (aboBoNT-A) is approved worldwide in indications related to excessive muscle activity, including focal upper and lower limb spasticity. Objective We evaluated real-world outcomes associated with on-label use of aboBoNT-A for adult spasticity UK routine clinical practice. Methods This was a multicenter, retrospective, 52-week study analyzing data from patients who had received ≥1 injection line the Summary Product Characteristics. Primary endpoints were average...
1. The incorporation of [35S]methionine into protein was investigated in tissue minces from different regions the rabbit epididymis incubated vitro. Rates synthesis were order: epididymal 2-5 greater than region 7 6 1 8 ductus deferens ductuli efferentes. 2. Separation labelled proteins on polyacrylamide gels containing sodium dodecyl sulphate followed by fluorography revealed that one (mol.wt. approx. 90 000) characteristic 1, four (one mol.wt. 54 000 and three 20 synthesized principally...
Three hormonally regulated proteins with mol.wts of 18 500, 19 000 and 23 have been shown to associate the plasma membrane spermatozoa during maturation in epididymis. All three showed some alpha-lactalbumin-like activity, suggesting that they may act concert epididymal glycosidases glycosyltransferases regulate modification sugars on membrane-bound glycoproteins.
Improving pandemic planning and preparedness is a challenge in Europe, diverse region whose regional bodies (the Regional Office for Europe of the World Health Organization [WHO], European Commission Centre Disease Prevention Control) have overlapping roles responsibilities.European indicators were used to develop an assessment tool procedure based on 2005 global WHO checklist preparedness. These then applied Member States WHO's Region, initially as part structured national assessments...
Native mass spectrometry (nMS) has emerged as a key analytical tool to study the organizational states of proteins and their complexes with both endogenous exogenous ligands. Specifically, for membrane proteins, it provides dimension determine identity bound lipids decipher effects on observed structural assembly. We recently developed an approach directly from intact tunable lipid membranes where biophysical properties its compositions can be customized. Extending this, we use our...
ABSTRACT Experiments have been carried out to identify proteins on boar spermatozoa that bind components of the zona pellucida. Polypeptides in sodium deoxycholate extracts and whole seminal plasma separated by dodecyl sulphate-polyacrylamide gel electrophoresis, transferred onto nitrocellulose sheet electroblotting probed with 125I-labelled heat-solubilized pellucida from pig oocytes or ovulated eggs. Zona bound avidly consistently a polypeptide Mr 53 000 blots capacitated noncapacitated...
Boar sperm acrosin isolated by affinity chromatography on p-(p'-aminophenoxypropoxy)benzamidine linked to Sepharose was tested for its proteolytic effect the zona pellucida of freshly ovulated pig eggs. During 1 h in a physiological medium there no observable change morphology but subsequent 125I labelling structure followed electrophoretic analysis revealed that had asserted limited and selective proteolysis.