A5060714245- Autophagy in Disease and Therapy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Cannabis and Cannabinoid Research
- CAR-T cell therapy research
- Legionella and Acanthamoeba research
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Helicobacter pylori-related gastroenterology studies
- Virus-based gene therapy research
- Immune Response and Inflammation
- Parkinson's Disease Mechanisms and Treatments
- Biomedical Text Mining and Ontologies
- HIV Research and Treatment
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Cancer-related gene regulation
- thermodynamics and calorimetric analyses
- Microbial Natural Products and Biosynthesis
- Galectins and Cancer Biology
- Vitamin K Research Studies
- Bacterial biofilms and quorum sensing
- Probiotics and Fermented Foods
- Carbohydrate Chemistry and Synthesis
- Microtubule and mitosis dynamics
University of California, Berkeley
2022-2024
Hurley Medical Center
2023-2024
QB3
2022-2024
Research Network (United States)
2023-2024
California University of Pennsylvania
2024
University of Oregon
2019-2021
Activation of PINK1 and Parkin in response to mitochondrial damage initiates a that includes phosphorylation RAB7A at Ser72. Rubicon is binding negative regulator autophagy. The structure the Rubicon:RAB7A complex suggests Ser72 would block binding. Indeed, vitro by TBK1 abrogates Pacer, positive autophagy, has an RH domain with basic triad predicted bind introduced phosphate. Consistent this, Pacer-RH binds phosho-RAB7A but not unphosphorylated RAB7A. In cells, depolarization reduces...
The gastric pathogen Helicobacter pylori requires a noncanonical cytosolic chemoreceptor transducer-like protein D (TlpD) for efficient colonization of the mammalian stomach. Here, we reconstituted complete chemotransduction signaling complex in vitro with TlpD and chemotaxis (Che) proteins CheW CheA, enabling quantitative assays potential ligands. We found that is selectively sensitive at micromolar concentrations to bleach (hypochlorous acid, HOCl), potent antimicrobial produced by...
The adaptor protein (AP) complexes not only form the inner layer of clathrin coats but also have clathrin-independent roles in membrane traffic whose mechanisms are unknown. HIV-1 Nef hijacks AP-1 to sequester major histocompatibility complex class I (MHC-I), evading immune detection. We found that AP-1:Arf1:Nef:MHC-I forms a coat on tubulated membranes without and determined its structure. assembles via Arf1 dimer interfaces. AP-1-positive tubules enriched cells upon knockdown. localizes...
Bacteria that colonize animals must overcome, or coexist, with the reactive oxygen species products of inflammation, a front-line defense innate immunity. Among these is neutrophilic oxidant bleach, hypochlorous acid (HOCl), potent antimicrobial plays primary role in killing bacteria through nonspecific oxidation proteins, lipids, and DNA. Here, we report response to increasing HOCl levels, Escherichia coli regulates biofilm production via activation diguanylate cyclase DgcZ. We identify...
Methods used in this paper.
For quantification of PINK-PARKIN mitophagy
Production of pseudotyped virus an subsequent transduction.
Fungal polysaccharide monooxygenases (PMOs) oxidatively degrade cellulose and other carbohydrate polymers via a mononuclear copper active site using either O
SUMMARY The gastric pathogen Helicobacter pylori requires a non-canonical cytosolic chemoreceptor transducer-like protein D (TlpD) for efficient colonization of the mammalian stomach. Here we reconstituted complete chemotransduction signaling complex in vitro with TlpD and chemotaxis proteins CheW CheA, enabling quantitative assays potential ligands. We found that is selectively sensitive at micromolar concentrations to bleach (hypochlorous acid, HOCl), potent antimicrobial produced by...
SUMMARY Activation of PINK1 and Parkin in response to mitochondrial damage initiates a cytoprotective mitophagy that includes phosphorylation RAB7A at Ser72. Rubicon is binding protein acts as negative regulator autophagy. The structure the Rubicon-RAB7A complex suggests Ser72 would block binding. Indeed, vitro by TBK1 abrogates Pacer, positive autophagy, has an RH domain with basic triad predicted bind introduced phosphate. Consistent this, Pacer-RH binds phosho-RAB7A but not...
ABSTRACT The reactive oxygen species produced during inflammation through the neutrophilic respiratory burst play profound roles in combating bacterial pathogens and regulating microbiota. Among these, oxidant bleach, hypochlorous acid (HOCl), is most prevalent strongest oxidizer kills bacteria non-specific oxidation of proteins, lipids, DNA. Thus, HOCl can be viewed as a host-specific cue that conveys important information about what physiology lifestyle programs may required for successful...
Phosphorylation of Rab7 at S72 using TBK1
1:1 binding of Rubicon and Rab7
Pule-chaseable method for quantifying autophagy flux
Growth in DMEM supplemented with FBS and antibiotics
Using the Rubicon RH - Rab7 crystal structure as a template (6wcw)
Production of pseudotyped virus an subsequent transduction.
Affinity purification followed by size exclusion chromatography.