A5004119274- Neonatal Respiratory Health Research
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Fatty Acid Research and Health
- Birth, Development, and Health
- Neuroscience of respiration and sleep
- Metabolism and Genetic Disorders
- Neonatal Health and Biochemistry
- Cellular transport and secretion
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Adipose Tissue and Metabolism
- Blood disorders and treatments
- Protein Kinase Regulation and GTPase Signaling
- Peroxisome Proliferator-Activated Receptors
- Coral and Marine Ecosystems Studies
- Inhalation and Respiratory Drug Delivery
- Marine and coastal plant biology
- Neuroblastoma Research and Treatments
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
University of Southampton
2005-2021
Southampton General Hospital
1993-2019
NIHR Southampton Respiratory Biomedical Research Unit
2012
Centre for Inflammation Research
2005-2006
St. Jude Children's Research Hospital
2000
University of Tennessee at Knoxville
2000
Imperial College London
2000
The activity of CTP:phosphocholine cytidylyltransferase, a rate-limiting enzyme in phosphatidylcholine biosynthesis, is modulated by its interaction with lipid bilayers [Kent, C. (1997) Biochim. Biophys. Acta 1348, 79-90]. Its regulation central importance the maintenance membrane homeostasis. Here we show evidence that stored curvature elastic stress membrane's monolayer modulates cytidylyltransferase. Our results how purely physical feedback signal could play key role control synthesis.
Phosphatidylinositol transfer proteins (PITPs) are versatile required for signal transduction and membrane traffic. The best characterized mammalian PITPs the Class I PITPs, PITPα (PITPNA) PITPβ (PITPNB), which single domain with a hydrophobic cavity that binds phosphatidylinositol (PI) or phosphatidylcholine molecule. In this study, we report lipid binding properties of an uncharacterized soluble PITP, protein, cytoplasmic 1 (PITPNC1) (alternative name, RdgBβ), II family. We show protein...
Abstract Climate change-induced global warming threatens the survival of key ecosystems including shallow water coral reefs. Elevated temperatures can disrupt normal physiological functioning photosynthetic organisms by altering fluidity and permeability chloroplast membranes that is defined regulated their lipid composition. Since habitat-forming reef corals rely on obligatory symbiosis with dinoflagellates family Symbiodiniaceae , heat stress response be expected to strongly influenced...
Chromatin-associated phospholipids are well recognized. A report that catalytically active endonuclear CTP:choline-phosphate cytidylyltransferase α is necessary for cell survival questions whether endonuclear, CDP-choline pathway phosphatidylcholine synthesis may occur <i>in situ</i>. We chromatin from human IMR-32 neuroblastoma cells possesses such a biosynthetic pathway. First, membrane-free nuclei retain all three enzymes in proportions comparable with the content of chromatin-associated...
Late pregnancy in the rat (gestational ages 16-21 days) was accompanied by a specific increase hepatic phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecular species containing C16:0 at sn-1 position polyunsaturated essential fatty acids (PUFA), particular C22:6(n-3), sn-2 position. Incorporation of either CDP:[Me-14C]choline or CDP:[1,2-14C]-ethanolamine into microsomal PC PE vitro greater term than non-pregnant animals, suggesting modifications to composition diacylglycerol...
ATP-binding cassette A3 (ABCA3) is a lipid transport protein required for synthesis and storage of pulmonary surfactant in type II cells the alveoli. Abca3 was conditionally deleted respiratory epithelial (Abca3(Δ/Δ)) vivo. The majority mice which alveolar died shortly after birth from distress related to deficiency. Approximately 30% Abca3(Δ/Δ) survived birth. Surviving developed emphysema absence significant inflammation. Staining lung tissue mRNA isolated demonstrated that ∼50% lacked...
Pulmonary inflammation is associated with altered lipid synthesis and clearance related to diabetes, obesity, various inherited metabolic disorders. In many tissues, lipogenesis regulated at the transcriptional level by activity of sterol-response element-binding proteins (SREBP). The role SREBP activation in regulation metabolism lung was assessed mice which both Insig1 Insig2 genes, encoding that bind inhibit SREBPs endoplasmic reticulum, were deleted alveolar type 2 cells. Although...
Deuterated choline‐ d 9 labelling of IMR‐32 cells enabled comparison the molecular specificities whole cell and endonuclear phosphatidylcholine synthesis after 96 h polyunsaturated fatty acid supplementation. Surprisingly, while remodelling reflected a pattern accretion, saturated pool was only transiently labelled with polyunsaturates. Periodic accumulations lipid second messenger diacylglycerol, mobilised from unsaturated phosphatidylinositol or phosphatidylcholine, accompany...
PPARγ (peroxisome proliferator-activated receptor γ) is a ligand-activated transcription factor that responds to 15dPGJ2 (15-deoxy-Δ12,14-prostglandin J2). 15dPGJ2, in vitro, halts neuroblastoma cell growth, but reported mechanisms vary. Here we evaluated the modulatory effects of endogenous serum lipid mitogens upon extent 15dPGJ2-induced growth inhibition and on precise cellular responses cells activation. We show specifically inhibited both complete delipidated media. was accompanied by...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES