A5054123949- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Plant biochemistry and biosynthesis
- Gene Regulatory Network Analysis
- PI3K/AKT/mTOR signaling in cancer
- Chromosomal and Genetic Variations
- Advanced Proteomics Techniques and Applications
- DNA Repair Mechanisms
- Plant nutrient uptake and metabolism
- RNA and protein synthesis mechanisms
Charles University
2022-2024
Oxidative stress is associated with cardiovascular and neurodegenerative diseases, diabetes, cancer, psychiatric disorders aging. In order to counteract, eliminate and/or adapt the sources of stress, cells possess elaborate stress-response mechanisms, which also operate at level regulating transcription. Interestingly, it becoming apparent that metabolic state cell certain metabolites can directly control epigenetic information gene expression. fission yeast Schizosaccharomyces pombe,...
ABSTRACT CSL proteins [named after the homologs CBF1 (RBP-Jκ in mice), Suppressor of Hairless and LAG-1] are conserved transcription factors found animals fungi. In fission yeast Schizosaccharomyces pombe, they regulate various cellular processes, including cell cycle progression, lipid metabolism adhesion. bind to DNA through their N-terminal Rel-like domain central β-trefoil domain. Here, we investigated importance binding for protein functions yeast. We created mutants with disrupted that...
Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC (cohesin, condensin and SMC5/6) play key roles in cohesion, condensation, replication, transcription DNA repair. Their physical binding to requires accessible chromatin.
Mitosis is a crucial stage in the cell cycle, controlled by vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe demonstrates catastrophic mitotic phenotypes due mutations or drug treatments. One factors provoking mitosis disturbed lipid metabolism, resulting from, for example, acetyl-CoA/biotin carboxylase (cut6), fatty acid synthase (fas2, also known as lsd1) transcriptional regulator metabolism (cbf11) genes, well...
ABSTRACT Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides powerful platform to study the intricate regulatory mechanisms governing these fundamental processes. In S. , Cbf11 Mga2 proteins transcriptional activators of non-sterol metabolism genes, with also known as regulator. Despite sharing common set target little was about their functional relationship. This reveals that...
Within a eukaryotic cell, both lipid homeostasis and faithful cell cycle progression are meticulously orchestrated. The fission yeast Schizosaccharomyces pombe provides powerful platform to study the intricate regulatory mechanisms governing these fundamental processes. In S. pombe, Cbf11 Mga2 proteins transcriptional activators of non-sterol metabolism genes, with also known as regulator. Despite sharing common set target little was about their functional relationship. This reveals that...
ABSTRACT Mitosis is a critical stage in the cell cycle, controlled by vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe may demonstrate catastrophic mitotic phenotypes due mutations or drug treatments. One factors provoking mitosis disturbed lipid metabolism, resulting from e.g. acetyl-CoA/biotin carboxylase ( cut6 ), fatty acid synthase fas2/lsd1 transcriptional regulator metabolism cbf11 ) genes, as well treatment...
ABSTRACT CSL (CBF1/RBP-Jκ/Suppressor of Hairless/LAG-1) proteins are conserved transcription factors found in animals and fungi. In fission yeast, they regulate various cellular processes, including cell cycle progression, lipid metabolism, adhesion. bind to DNA through their N-terminal Rel-like domain central beta-trefoil domain. Here, we investigated the importance binding for functions yeast Schizosaccharomyces pombe . We created mutants with disrupted that vast majority depend on intact...
ABSTRACT Oxidative stress is associated with cardiovascular and neurodegenerative diseases, diabetes, cancer, psychiatric disorders aging. In order to counteract, eliminate and/or adapt the sources of stress, cells possess elaborate stress-response mechanisms, which also operate at level regulating transcription. Interestingly, it becoming apparent that metabolic state cell certain metabolites can directly control epigenetic information gene expression. fission yeast Schizosaccharomyces...
Abstract Structural Maintenance of Chromosomes (SMC) complexes are molecular machines driving chromatin organization at higher levels. In eukaryotes, three SMC (cohesin, condensin, and SMC5/6) play key roles in cohesion, condensation, replication, transcription DNA repair. Here, we performed a genetic screen fission yeast to identify novel factors required for SMC5/6 binding DNA. We identified 79 genes which histone acetyltransferases (HATs) were the most represented. Genetic phenotypic...