A5054196894- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Catalysis for Biomass Conversion
- Biochemical and Molecular Research
- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Lignin and Wood Chemistry
- Chemical Synthesis and Reactions
- Enzyme Structure and Function
- Fungal and yeast genetics research
- Click Chemistry and Applications
- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Biochemical and biochemical processes
- Asymmetric Synthesis and Catalysis
- RNA modifications and cancer
- Synthetic Organic Chemistry Methods
- Sulfur-Based Synthesis Techniques
- Calcium signaling and nucleotide metabolism
Dalian Institute of Chemical Physics
2016-2025
Chinese Academy of Sciences
2015-2024
Dalian University
2014-2024
Dalian University of Technology
2023-2024
Dalian National Laboratory for Clean Energy
2010-2020
Northeast Agricultural University
2020
The University of Texas at Austin
2001-2013
University of Chinese Academy of Sciences
2008-2013
Research Center for Eco-Environmental Sciences
2010
In-Q-Tel
2009
Acid in ionic liquid was demonstrated as an efficient system for hydrolysis of lignocellulosic materials with improved total reducing sugars (TRS) yield under mild conditions. TRS yields were up to 66%, 74%, 81% and 68% corn stalk, rice straw, pine wood bagasse, respectively, C4mimCl the presence 7 wt% hydrogen chloride at 100 °C atmospheric pressure within 60 min. Different combinations between liquids, such C6mimCl, C4mimBr, AmimCl, C4mimHSO4, SbmimHSO4, acids, including sulfuric acid,...
Triacylglycerols are among the most attractive alternative raw materials for biofuel development. Current oil plant-based technologies limited in terms of triacylglycerol production capacity and rate. These limitations may be circumvented by biotransformation carbohydrates into lipids; however, our understanding microbial oleaginicity remains limited. Here we present results a multi-omic analysis Rhodosporidium toruloides, robust triacylglycerol-producing fungus. The assembly genome...
Microbial production can be advantageous over the extraction of phytoterpenoids from natural plant sources, but it remains challenging to rationally and rapidly access efficient pathway variants. Previous engineering attempts mainly focused on mevalonic acid (MVA) or methyl-d-erythritol phosphate (MEP) pathways responsible for generation precursors terpenoids biosynthesis, potential interactions between diterpenoids synthases were unexplored. Miltiradiene, product stepwise conversion...
Abstract A novel method for cellulose hydrolysis catalyzed by mineral acids in the ionic liquid 1‐butyl‐3‐methylimidazolium chloride ([C 4 mim]Cl) has been developed that facilitates of with dramatically accelerated reaction rates at 100 °C under atmospheric pressure and without pretreatment.
Cytochrome P450 enzymes (CYPs) play major roles in generating highly functionalized terpenoids, but identifying the exact biotransformation step(s) catalyzed by plant CYP terpenoid biosynthesis is extremely challenging. Tanshinones are abietane-type norditerpenoid naphthoquinones that main lipophilic bioactive components of Chinese medicinal herb danshen ( Salvia miltiorrhiza ). Whereas diterpene synthases responsible for conversion E,E,E )-geranylgeranyl diphosphate into abietane...
Abstract Biodiesel is a renewable fuel conventionally prepared by transesterification of pre‐extracted vegetable oils and animal fats all resources with methanol, catalyzed strong acids or bases. This paper reports on novel biodiesel production method that features acid‐promoted direct methanolysis cellular biomass oleaginous yeasts filamentous fungi. The process was optimized for tuning operation parameters, such as methanol dosage, catalyst concentration, reaction temperature time. Up to...
Cytochromes P450 (CYPs) play a key role in generating the structural diversity of terpenoids, largest group plant natural products. However, functional characterization CYPs has been challenging because expansive families found genomes, diverse reactivity and inaccessibility their substrates Here we present two CYPs, CYP76AH3 CYP76AK1, which act sequentially to form bifurcating pathway for biosynthesis tanshinones, oxygenated diterpenoids from Chinese medicinal Danshen (Salvia miltiorrhiza)....
Heteroatom-participated lignin depolymerization for heterocyclic aromatic compounds production is of great importance to expanding the product portfolio and meeting value-added biorefinery demand, but it also particularly challenging. In this work, synthesis pyrimidines from β-O-4 model compounds, most abundant segment in lignin, mediated by NaOH through a one-pot multi-component cascade reaction reported. Mechanism study suggests that transformation starts NaOH-induced deprotonation Cα-H...
Abstract Co‐fermentation of lignocellulose‐based carbohydrates is a potential solution to improve the economics microbial lipid production. In present paper, experiments were performed optimize media composition for production by oleaginous yeast Lipomyces starkeyi AS 2.1560 through co‐fermentation glucose and xylose (2 : 1 wt/wt). Statistical screening nine variables was Plackett–Burman design. Three factors, namely mixed sugar, extract FeSO 4 , found as significant components influencing...
Abstract Direct conversion of carbohydrates into 5‐hydroxymethylfurfural (HMF) catalyzed by germanium(IV) chloride in ionic liquids has been investigated search an efficient and environmentally friendly process. Monosaccharides D ‐fructose ‐glucose, disaccharides sucrose maltose, even the polysaccharide cellulose were successfully converted HMF with good yields under mild conditions (yield up to 92 % 5 min case fructose). The structure liquids, catalyst loading, reaction temperature water...
Abstract A clean, facile, and environment‐friendly catalytic method has been developed for the conversion of furfuryl alcohol into alkyl levulinates making use novel solid catalyst methylimidazolebutylsulfate phosphotungstate ([MIMBS] 3 PW 12 O 40 ). The is an organic–inorganic hybrid material, which consists organic cation inorganic anion. study optimizing reaction conditions such as time, temperature loading performed. Under optimal conditions, a high n ‐butyl levulinate yield up to 93 %...
Abstract Background Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence both hexoses and pentoses. Thus, identification microorganisms capable efficient simultaneous utilization glucose xylose is pivotal to improving this process. Results In study, we found that oleaginous yeast strain Trichosporon cutaneum AS 2.571 assimilated simultaneously, accumulated intracellular lipid up 59...
Acetic acid is routinely generated during lignocelluloses degradation, syngas fermentation, dark hydrogen fermentation and other anaerobic bioprocesses. Acetate stream commonly regarded as a by-product detrimental to microbial cell growth. Conversion of acetate into lipids by oleaginous yeasts may be good choice turn the treasure. Ten well-known were evaluated for lipid production on under flask culture conditions. It was found that all those could use production. In particular, Cryptococcus...