A5052648336- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- Ion channel regulation and function
- COVID-19 Clinical Research Studies
- Ecology and Vegetation Dynamics Studies
- Plant and animal studies
- Cardiovascular Effects of Exercise
- 3D Printing in Biomedical Research
- Atrial Fibrillation Management and Outcomes
- SARS-CoV-2 and COVID-19 Research
- Receptor Mechanisms and Signaling
- Sensory Analysis and Statistical Methods
- Long-Term Effects of COVID-19
- Tissue Engineering and Regenerative Medicine
- Neurological disorders and treatments
- Pluripotent Stem Cells Research
- Thermal Radiation and Cooling Technologies
- Mesenchymal stem cell research
- Cardiac Arrhythmias and Treatments
- Pericarditis and Cardiac Tamponade
- Cellular Mechanics and Interactions
- Planetary Science and Exploration
- Graphite, nuclear technology, radiation studies
- Thyroid Disorders and Treatments
- Quantum Electrodynamics and Casimir Effect
Stanford University
2024
National Institute of Aerospace
2024
University of Wisconsin–Madison
2019-2023
Medistem (Panama)
2020-2021
City of Knowledge
2021
Hackensack Meridian Health
2021
Aidan Foundation
2020
University of Michigan
2014-2018
Union County College
2018
Brown University
2018
ABSTRACT Priority effects, where the order and timing of species arrival influence assembly ecological communities, have been observed in a variety taxa habitats. However, genetic molecular basis priority effects remains unclear, hindering better understanding when will be strong. We sought to gain such an for nectar yeast Metschnikowia reukaufii commonly found our study plant, hummingbird‐pollinated Diplacus ( Mimulus ) aurantiacus . In this M can experience strong it reaches flowers after...
Background Atrial fibrillation often occurs in the setting of hypertension and associated atrial dilation with pathologically increased cardiomyocyte stretch. In dilation, mechanoelectric feedback has been linked to development ectopic beats that trigger paroxysmal mainly originating from pulmonary veins ( PVs ). However, precise mechanisms remain poorly understood. Methods Results We identify mechanosensitive, swelling‐activated chloride ion channels I C l,swell ) as a crucial component...
Truncation mutations in cardiac myosin binding protein C (cMyBP-C) are common causes of hypertrophic cardiomyopathy (HCM). Heterozygous carriers present with classical HCM, while homozygous early onset HCM that rapidly progress to heart failure. We used CRISPR-Cas9 introduce heterozygous (cMyBP-C+/−) and (cMyBP-C−/−) frame-shift into MYBPC3 human iPSCs. Cardiomyocytes derived from these isogenic lines were generate micropatterns engineered tissue constructs (ECTs) characterized for...
Hypertrophic cardiomyopathy (HCM) is triggered mainly by mutations in genes encoding sarcomeric proteins, but a significant proportion of patients lack genetic diagnosis. We identified novel mutation the ryanodine receptor 2, RyR2-P1124L, patient from genotype-negative HCM cohort. The aim this study was to determine whether RyR2-P1124L triggers functional and structural alterations isolated RyR2 channels whole hearts. found that P1124L induces conformational changes SPRY2 domain RyR2....
Abstract Tailoring the emission spectra of a thermophotovoltaic (TPV) emitter away from that blackbody has potential to minimize transmission and thermalization loss in photovoltaic receiver. Selective TPV emitters can lead solar energy conversion with efficiency greater than Shockley–Queisser limit facilitate generation useful waste heat. A new design is introduced radically tune thermal leverages interplay between two resonant phenomena simple planar nanostructures—absorption weakly...
Abstract Background Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported hUC-MSCs one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2), a key protein for viral infection along with Transmembrane Serine Protease (TMPRSS2). The purpose this study was quantify the expression ACE2 and TMPRSS2 in lots multiple donors using...
Background: The mechanisms underlying spontaneous atrial fibrillation (AF) associated with ischemia/infarction are incompletely elucidated. Here, we investigate the AF in an ovine model of left myocardial infarction (LAMI). Methods and Results: LAMI was created by ligating branch anterior descending coronary artery. ECG loop recorders were implanted to monitor episodes. In 7 sheep, dantrolene—a ryanodine receptor blocker—was administered vivo during 8-day observation period (LAMI-D, 2.5...
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is characterized by an arrhythmogenic mechanism involving disruption of calcium handling. This genetic disease can lead to sudden death in children and young adults during physical or emotional stress. Prior CPVT studies have focused on handling, but mechanical functionality has rarely been investigated vitro . In this research we combine stem cell-derived cardiomyocytes from a patient (RyR2-H2464D mutation) healthy familial...
Abstract Priority effects, where the order and timing of species arrival influence assembly ecological communities, have been observed in a variety taxa habitats. However, genetic molecular basis priority effects remains unclear most cases, hindering mechanistic understanding effects. We sought to gain such for common nectar yeast Metschnikowia reukaufii , which engages strong with other yeast, including M. rancensis another commonly found our study plant, hummingbird-pollinated Diplacus (...
Priority effects, where the order and timing of species arrival influence assembly ecological communities, have been observed in a variety taxa habitats. However, genetic molecular basis priority effects remains unclear most cases, hindering mechanistic understanding effects. We sought to gain such for common nectar yeast Metschnikowia reukaufii, which engages strong with other yeast, including M. rancensis, another commonly found our study plant, hummingbird-pollinated Diplacus (Mimulus)...
Cardiac tamponade is a medical emergency and must be managed promptly, reaching diagnosis imperative to prevent recurrence. Herein, we present case of young female patient that presented with progressive shortness breath abdominal distension was found have cardiac the finding elevation blood tumor marker, CA-125, in setting nonadherence thyroid replacement therapy. She by surgical pericardial window paracentesis, hormones leading resolution ascites. CA-125 associated myxedema ascites due...
Background: Mutations in the gene encoding cardiac sodium channel (SCN5A) have been implicated arrhythmia syndromes including Brugada (BrS) and long-QT type 3 syndromes. Also, wide spectrums of mixed phenotypes, known as “overlap syndromes”, associated to SCN5A mutations. The contributions single nucleotide polymorphisms (SNPs) add more complexity disease phenotype. Here we report a missense mutation (T1708N) three Hispanic families with history sudden death (SCD) extensive variability...
Introduction: Modeling heart diseases using cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) enables direct study of cellular and molecular mechanisms. However, the variable phenotypes individual has limited reproducibility contractile measurements. We hypothesized that variability in myofibrillar development hiPSC-CMs influences kinetics could be controlled for by visualizing quantifying myofilaments vitro . Methods Results: Control hiPSCs were differentiated...
Hypertrophic cardiomyopathy (HCM) is an inherited cardiac disease that can cause sudden arrest, diastolic dysfunction and heart failure. Mutations in myosin binding protein-C (cMyBP-C), a regulator of contractility, are among the most prevalent causes HCM. cMyBP-C truncation mutations may affect as many 1 1,600 Americans. Though murine models ablation have aided understanding role health disease, they limited by differences physiology between mice humans. iPSC technology now allows...
The mechanism of MYBPC3 (encoding cardiac myosin binding protein C, MyBP-C) truncation mutations, the most common genetic cause hypertrophic cardiomyopathy, has been incompletely resolved. We hypothesized that truncating mutations myofibrillar assembly defects and/or contractile dysfunction. Methods and Results: Control iPSCs were CRISPR/Cas9-edited to create cell lines with homozygous (homCT) heterozygous (hetCT) C-terminal promoter knock-out (hetPROM). HetCT was further edited add an...
Abstract Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported hUC-MSCs one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2), a key protein for viral infection along with Transmembrane Serine Protease (TMPRSS2). In this report, the expression ACE2 and TMPRSS2 was analyzed in 24 lots different donors via quantitative polymerase...
Abstract BackgroundMesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have immunomodulatory properties that are of interest to treat novel coronavirus disease 2019 (COVID-19). Leng et al. recently reported hUC-MSCs one donor negatively expressed Angiotensin-Converting Enzyme 2 (ACE2), a key protein for viral infection along with Transmembrane Serine Protease (TMPRSS2). MethodsExpression ACE2 and TMPRSS2 was analyzed in 24 lots fromWharton's jelly via quantitative polymerase...