A5019709390- Mechanical Circulatory Support Devices
- Cardiac Arrest and Resuscitation
- Transplantation: Methods and Outcomes
- Cardiac Ischemia and Reperfusion
- Organ Transplantation Techniques and Outcomes
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Organ Donation and Transplantation
- Congenital Diaphragmatic Hernia Studies
- Congenital Heart Disease Studies
- Renal Transplantation Outcomes and Treatments
- Cardiac and Coronary Surgery Techniques
- Organ and Tissue Transplantation Research
- Cardiac Structural Anomalies and Repair
- Liver Disease and Transplantation
- Pulmonary Hypertension Research and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Congenital Anomalies and Fetal Surgery
- Neuroscience of respiration and sleep
- Nitric Oxide and Endothelin Effects
- Tissue Engineering and Regenerative Medicine
- Anesthesia and Neurotoxicity Research
- Central Venous Catheters and Hemodialysis
- Electrospun Nanofibers in Biomedical Applications
- Electrochemical sensors and biosensors
University of Michigan–Ann Arbor
2015-2024
Michigan Medicine
2010-2023
Regional West Medical Center
2023
Michigan United
2006-2023
Ann Arbor Center for Independent Living
2018
Columbia University
2015-2018
Brown University
2018
West German Heart and Vascular Center Essen
2018
McGill University
2018
The University of Tokyo
2018
Extracorporeal support (ECS) during organ procurement from donors after circulatory determination of death (DCDD) could increase the number donor organs and decrease posttransplant complications. This study reports experience a large transplant center with controlled DCDD.A retrospective review all potential controlled-DCDD cases between October 1, 2000 July 31, 2013 was performed. We focused on methods, ethical practical issues, recipient outcome data procured transplanted in our...
Vascularized composite allografts, particularly hand and forearm, have limited ischemic tolerance after procurement. In bilateral transplantations, this demands a 2 team approach expedited transfer of the allograft, limiting recovery to small geographic area. Ex situ perfusion may be an alternative allograft preservation method extend survival time. This is short report 5 human limbs maintained for 24 hours with ex perfusion.Upper were procured from brain-dead organ donors. Following...
Organ perfusion systems have successfully been applied in solid organ transplantations. Their use limb transplantation and replantation has not widely investigated. In this study, we tested the potential for ex situ system to prolong allograft viability a swine forelimb amputation/replantation model.Fourteen were used. group 1 (n = 4), perfused 4 amputated limbs 12 hours using warm (27 °C-32 °C) autologous blood. Group 2 3) served as cold preservation control group, preserving 6 at °C. All...
Although total body perfusion with extracorporeal life support (ECLS) can be maintained for weeks, individual organ beyond 12 hours has yet to achieved clinically. Normothermic ex situ heart (ESHP) offers the potential prolonged cardiac preservation. We developed an ESHP system study effect of perfusate variables on preservation, ultimate goal extending ≥24 hours. Forty porcine hearts were perfused a target Hearts that electromechanical activity and had <3× increase in vascular resistance...
Artificial lungs have been used in the clinic for multiple decades to supplement patient pulmonary function. Recently, small-scale microfluidic artificial (μAL) demonstrated with large surface area blood volume ratios, biomimetic flow paths, and pressure drops compatible pumpless operation. Initial devices rates μl/min ml/min range exhibited excellent gas transfer efficiencies; however, current manufacturing techniques may not be suitable scaling up human applications. Here, we present a new...
A new portable gas phase nitric oxide (NO) generator is described for potential applications in inhaled NO (INO) therapy and during cardiopulmonary bypass (CPB) surgery. In this system, produced at the surface of a large-area mesh working electrode by electrochemical reduction nitrite ions presence soluble copper(II)-ligand electron transfer mediator complex. The generated then transported into either direct purging with nitrogen/air or via circulating electrolyte/nitrite solution through...
Donors after cardiac death (DCD) could increase the organ pool. Data supports good long‐term renal graft survival. However, DCDs are <10% of deceased donors in United States, due to delayed function, and primary nonfunction. These complications minimized by extracorporeal support (ECS‐DCD). This study assesses immediate acute function from different donor types. kidneys were recovered conventional rapid recovery or ECS, transplanted into nephrectomized healthy swine. Warm ischemia 10 30 min...
An extracorporeal artificial placenta would change the paradigm of treating extremely premature infants. We hypothesized that a venovenous life support (VV-ECLS) maintain fetal circulation, hemodynamic stability, and adequate gas exchange for 24 hours. A near-term neonatal lamb model (130 days; term = 145 days) was used (n 9). The right jugular vein cannulated VV-ECLS outflow, an umbilical inflow. circuit included peristaltic roller pump 0.5 m(2) hollow fiber oxygenator. Lambs were...
A novel electrochemically controlled release method for nitric oxide (NO) (based on electrochemical reduction of nitrite ions) is combined with an amperometric oxygen sensor within a dual lumen catheter configuration the continuous in vivo sensing partial pressure (PO2) blood. The on-demand NO generation/release shown to be fully compatible PO2 sensing. performance sensors evaluated rabbit veins and pig arteries 7 21 h, respectively. Overall, releasing measure both venous arterial values...
Prolonged normothermic ex vivo heart perfusion could transform cardiac transplantation. To help identify perfusate components that might enable long-term perfusion, we evaluated the effects of cross-circulated whole blood and plasma from a live paracorporeal animal on donor porcine hearts preserved via perfusion. Standard (SP; n = 40) utilized red cell/plasma Langendorff technique for goal 12 hours. Cross-circulation groups used similar circuit with addition venous (XC-blood; 6) or filtered...
Cell therapy for the treatment of renal failure in acute setting has proved successful, with therapeutic impact, yet development a sustainable, portable bioartificial kidney chronic to be realized. Challenges maintaining an anticoagulated blood circuit, typical platform solute clearance and support biological components, have posed major hurdle advancement this technology. This group developed Bioartificial Renal Epithelial System (BRECS) capable differentiated cell function while sustained...
Preservation of a donor heart for transplantation is limited to 6–8 hours. Based on our demonstration 12 hour perfusion with plasma cross circulation, this study aimed evaluate ex vivo (EVHP) up 72 hours using circulation (XC-plasma) from live, awake paracorporeal sheep (PCS). Six ovine hearts were perfused at rate 1 L/min PCS. Controls seven without circulation. Experiments electively ended hours, and epinephrine (0.1 mg) was delivered demonstrate hormonal responsiveness. All controls...
Nitric oxide-based biocompatibility strategy.
Background: The mechanisms underlying spontaneous atrial fibrillation (AF) associated with ischemia/infarction are incompletely elucidated. Here, we investigate the AF in an ovine model of left myocardial infarction (LAMI). Methods and Results: LAMI was created by ligating branch anterior descending coronary artery. ECG loop recorders were implanted to monitor episodes. In 7 sheep, dantrolene—a ryanodine receptor blocker—was administered vivo during 8-day observation period (LAMI-D, 2.5...
Cold static storage and normothermic ex vivo heart perfusion are routinely limited to 6 h. This report describes intermittent left atrial (LA) that allows cardiac functional assessment in a working mode.
Cold static storage (CSS) for up to 6 h is the gold standard in heart preservation. Although some hearts stored over have been transplanted, longer CSS times increased posttransplant morbimortality. Transmedics® Organ Care System (OCS™) only FDA-approved commercial system that provides an alternative using normothermic ex situ perfusion (NEHP) resting mode with aortic (Langendorff method). However, it also limited and lacks objective assessment of cardiac function. Developing a can perfuse...