A5020072392- Dialysis and Renal Disease Management
- Tissue Engineering and Regenerative Medicine
- Acute Kidney Injury Research
- Renal and related cancers
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Muscle and Compartmental Disorders
- Organ Donation and Transplantation
- Polymer Surface Interaction Studies
- Renal and Vascular Pathologies
- Corneal Surgery and Treatments
- Neonatal Respiratory Health Research
- Mechanical Circulatory Support Devices
- Sepsis Diagnosis and Treatment
- Vagus Nerve Stimulation Research
- Ocular Surface and Contact Lens
- Chronic Kidney Disease and Diabetes
- Natural product bioactivities and synthesis
- Connexins and lens biology
- Nanofabrication and Lithography Techniques
- Corneal surgery and disorders
- Mesenchymal stem cell research
- Biomedical Ethics and Regulation
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
Innovative BioTherapies (United States)
2012-2025
University of Michigan
2010-2024
University of Colorado Anschutz Medical Campus
2019-2023
University of Colorado Denver
2023
Michigan Medicine
2022-2023
Hospital Italiano de Buenos Aires
2017
Vanderbilt University
2004-2013
Michigan United
2011
University of Freiburg
2010
D'Youville College
1981
The acquisition of a mesenchymal phenotype is critical step in the metastatic progression epithelial carcinomas. Adherens junctions (AJs) are required for suppressing this epithelial-mesenchymal transition (EMT) but less known about role tight (TJs) process. Here, we investigated functions blood vessel epicardial substance (BVES, also as POPDC1 and POP1), an integral membrane protein that regulates TJ formation. BVES was found to be underexpressed all stages human colorectal carcinoma (CRC)...
Maintaining the three-dimensional architecture and cellular complexity of lung tissue ex vivo can enable elucidation molecular pathways underlying chronic pulmonary diseases. Precision-cut slices (PCLS) are one human-lung model with potential to support critical mechanistic studies early drug discovery. However, many report short culture times 7-10 days. Here, we systematically evaluated poly(ethylene glycol)-based hydrogel platforms for encapsulation PCLS. We demonstrated ability embedded...
Renal cell therapy has shown clinical efficacy in the treatment of acute renal failure (ARF) and promise for end-stage disease (ESRD) by supplementing conventional small solute clearance (hemodialysis or hemofiltration) with endocrine metabolic function provided cells maintained an extracorporeal circuit. A major obstacle widespread adoption this therapeutic approach is lack a cryopreservable system to enable distribution, storage, use at point care facilities. This report details design,...
Objective Septic shock has a clinical mortality rate approaching fifty percent. The major manifestations of sepsis are due to the dysregulation host's response infection rather than direct consequences invading pathogen. Central this initial immunologic is activation leukocytes and microvascular endothelium resulting in cardiovascular instability, lung injury renal dysfunction. Due primary role leukocyte syndrome, synthetic biomimetic membrane, called selective cytopheretic device (SCD), was...
Blood vessel epicardial substance (Bves) is a transmembrane adhesion protein that regulates tight junction (TJ) formation in variety of epithelia. The role TJs within epithelium extends beyond the mechanical properties. They have been shown to play direct regulation RhoA and ZONAB/DbpA, y-box transcription factor. We hypothesize Bves can modulate activation ZONAB/DbpA activity through its regulatory effect on TJ formation. Immortalized human corneal epithelial (HCE) cells were stably...
Abstract Cell directed therapy is an evolving therapeutic approach to treat organ dysfunction arising from hyperinflammation and cytokine storm by processing immune cells in extracorporeal circuit. To investigate the mechanism of action Selective Cytopheretic Device (SCD), vitro blood circuits were utilized interrogate several aspects immunomodulatory therapy. SCD activity due its effects on circulating neutrophils monocytes a low ionized calcium (iCa, Ca 2+ ) Activated adhere fibers...
Cell therapy for the treatment of renal failure in acute setting has proved successful, with therapeutic impact, yet development a sustainable, portable bioartificial kidney chronic to be realized. Challenges maintaining an anticoagulated blood circuit, typical platform solute clearance and support biological components, have posed major hurdle advancement this technology. This group developed Bioartificial Renal Epithelial System (BRECS) capable differentiated cell function while sustained...
<b><i>Introduction:</i></b> The manual single lumen alternating micro-batch hemodiafiltration (mSLAMB) system is a closed-loop dialysis designed to provide kidney support in emergency situations (e.g., fluid overload, hyperkalemia, acidemia). If done repeatedly small batches and at high flow rates, this was found achieve clearance levels comparable traditional renal replacement therapy. <b><i>Methods:</i></b> Using porcine model, uremic toxins...
Layer-by-layer (LBL) assembly of functionalized nanoparticles (FNPs) within a polyelectrolyte network is described (see image). The LBL-NP-assembly-based modification can be used to engineer surfaces with multiple functions such as controlled (multiple-) drug release distinct kinetics and enhanced contrast in clinical imaging modalities. This novel surface strategy expected accelerate the development bioresponsive coatings for implantable biomedical devices.
Renal cell therapy using the hollow fiber based renal assist device (RAD) improved survival time in an animal model of septic shock (SS) through amelioration cardiac and vascular dysfunction. Safety ability RAD to improve clinical outcomes was demonstrated a Phase II trial, which patients had high prevalence sepsis. Even with these promising results, delivery is hampered by manufacturing hurdles, including sourcing, large-scale manufacture, storage delivery. To address limitations,...
The bioartificial renal epithelial cell system (BRECS) is a cell-based device to treat acute kidney injury through therapy from an extracorporeal circuit. To enable widespread implementation of therapy, the BRECS was designed be cryopreserved as complete device, cryostored, cryoshipped end-use site, thawed and employed in therapeutic hemofiltration This strategy overcomes storage distribution issues that have been previous barriers therapy. Previous housings produced by computer numerical...
Background: Systemic inflammatory response syndrome (SIRS) can occur in association with cardiopulmonary bypass (CPB) surgery, resulting multiple organ dysfunction (MOD). Activated neutrophils have been implicated as major inciting factors this process. Neutrophil-depleting filters incorporated within the extracorporeal blood circuit during CPB developed and evaluated, inconsistent clinical results. Methods: A novel, biomimetic, selective cytopheretic device (SCD) was tested vitro a to...
Persistent corneal wounds result from numerous eye disorders, and to date, available treatments often fail accelerate reepithelialization, the key initial step in wound healing. To speed we explored a cell-transfer transplant method utilizing polydimethylsiloxane (PDMS) contact lenses deliver epithelial cells derived limbal explants directly within wound. Human primary an immortalized cell line (HCE-SV40) grew well on PDMS their morphology growth rates where similar grown tissue culture...
Stratum corneum, the outermost layer of skin, allows transport only low-molecular weight (<500) lipophilic solutes. Here, we report a surprising finding that avicins ( Av s), family naturally occurring glycosylated triterpenes with molecular > 2,000, exhibit skin permeabilities comparable to those small hydrophobic molecules, such as estradiol. Systematic fragmentation molecule shows deletion outer monoterpene results in 62% reduction permeability, suggesting an important role for this...
When polymeric nanoparticles (NPs) are formed by nanoprecipitation, which is a nucleation–growth process, the control over size requires changing polymer concentration or solvent composition. Here, we demonstrate that NP can be controlled independent of variables introducing polyelectrolyte (PE) in aqueous phase. PEs exhibit hydrogen bonding (H-bonding) yield reduction size, whereas do not possess this characteristic promote formation larger NPs. The observed effect attributed to diffusional...
Critically ill patients undergoing continuous renal replacement therapy (CRRT) have medical conditions requiring extensive pharmacotherapy. Continuous impacts drug disposition. Few data exist regarding dosing requirements with contemporary CRRT modalities and effluent rates. The practical limitations of pharmacokinetic studies numerous plasma samples, lack generalizability observations from specific prescriptions, highlight gaps in bedside assessment elimination individualized needs. We...