pH-sensitive hydrogels play an important role in controlled drug release applications and have the potential to impact management of wounds. In this study, we report fabrication novel carboxylated agarose/tannic acid hydrogel scaffolds cross-linked with zinc ions for pH-controlled tannic acid. The resulting exhibited negligible at neutral alkaline pH sustained acidic pH, where they also displayed maximum swelling. favorable antibacterial anti-inflammatory properties, a lack cytotoxicity...

Treatment of large defects requires the harvest fresh living bone from iliac crest. Harvest this limited supply is accompanied by extreme pain and morbidity. This has prompted exploration other alternatives to generate new using traditional principles tissue engineering, wherein harvested cells are combined with porous scaffolds stimulated exogenous mitogens morphogens in vitro and/or vivo. We now show that volumes can be engineered a predictable manner, without need for cell transplantation...

Porous polymeric media (polymer foams) are utilized in a wide range of applications, such as thermal and mechanical insulators, solid supports for catalysis, medical devices. A process the production polymer foams has been developed. This process, which is applicable to polymers, uses hydrocarbon particulate phase template precipitation subsequent pore formation. The use allows enhanced control over structure, porosity, other structural bulk characteristics foam. Polymer with densities low...

Significance Nanoparticles (NPs) can serve as containers for the targeting of therapeutics to tumors. Tumors comprise many cell types including endothelial cells that form blood vessels. Developing new strategies target information preferentially have major implications in development targeted therapeutics. We discovered charged polymers containing aromatic sulfonate pronounced affinity caveolae, which are highly expressed by cells. By engineering surface lipid NPs bear sulfonate-containing...

Extracellular soluble signals are known to play a critical role in maintaining neuronal function and homeostasis the CNS. However, CNS is also composed of extracellular matrix macromolecules glia support cells, contribution physical attributes these components maintenance regulation not well understood. Because possess well-defined topography, we theorize for topography development demonstrate that survival hippocampal neurons differentiation telencephalic neural stem cells modulated by...

Matrix-metalloproteinases 9 (MMP-9) belongs to the class of matrix metalloproteinases whose main function is degrade and remodel extracellular (ECM). MMP-9 has been shown be an integral part many diseases where modulation ECM a key step such as cancer, osteoporosis fibrosis. secreted latent pro-enzyme that requires activation in space. Therefore, identifying physiological molecular contexts, which can activate important. Acidification osteoclast-conditioned media pH 5 resulted fragment with...

Abstract This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree carboxylation, elastic modulus printed gels can be tuned over several orders magnitudes (5–230 Pa) while ensuring almost no change to shear viscosity (10–17 mPa) solution; thus enabling fabrication 3D structures made different mechanical domains under identical printing parameters...

Conducting films and pellets formed from β-substituted polypyrroles (see picture; R=H, CH3) erode under aqueous conditions in a pH-dependent manner, which allows erosion rates to be tailored through differences polymer side-chain structure. These conducting materials support the attachment, proliferation, differentiation of primary human cells, could lead use polymers variety new biomedical applications.

Bone marrow stromal cells (BMSC) are pluripotent progenitor that can regenerate different skeletal tissues in response to environmental signals. In this study, we used highly porous, structurally stable three-dimensional polymer foams conjunction with specific regulatory molecules selectively differentiate mammalian BMSC into either cartilaginous or bone-like tissues. Bovine were expanded monolayers and cultured on 5-mm-diameter, 2-mm-thick made of poly(lactic-co-glycolic acid) poly(ethylene...

Mechanical aspects of the cellular environment can influence cell function, and in this context hydrogels serve as an instructive matrix. Here we report that physicochemical properties derived from polysaccharides (agarose, κ-carrageenan) having α-helical backbone be tailored by inducing a switch secondary structure α-helix to β-sheet through carboxylation. This enables gel modulus tuned over four orders magnitude (G′ 6 Pa–3.6 × 10 4 Pa) independently polymer concentration molecular weight....

Three-dimensional (3-D) cultures of cancer cells can potentially bridge the gap between 2-D drug screening and in vivo xenografts. The objective this study was to characterize cellular extracellular matrix characteristics spheroids composed human lung epithelial (epi), pulmonary vascular endothelial (endo) cells, marrow-derived mesenchymal stems (MSCs).Spheroids epi/endo/MSCs, termed herein as synthetic tumor microenvironment mimics (STEMs), were prepared by hanging drop method. Cellular...

To study biological processes in vitro, biomaterials-based engineering solutions to reproduce the gradients observed tissues are necessary. We present a platform for 3D bioprinting of functionally graded biomaterials based on carboxylated agarose, bioink amendable by extrusion bioprinting. Using this bioink, objects with gradient stiffness and cell concentration were printed. Functionalization agarose maleimide moieties that react minutes cysteine-terminated cell-adhesion peptide allowed us...

The use of periosteum as a cell source for the in vitro engineering grafts articular cartilage repair requires development methods to obtain high viable numbers early stages culture. In this study, we demonstrate that addition mitogen, fibroblast growth factor-2 (FGF-2), during stage culture presence transforming factor-beta1 (TGF-beta1), significantly enhances proliferation, which results increased neo-cartilage formation at later stages. Periosteal explants were cultured within alginate or...

Treatment of full-thickness damage to hyaline cartilage is hampered by the limited availability autologous healthy and lengthy, cost-prohibitive cell isolation expansion steps associated with implantation (ACI). Here we report a strategy for de novo engineering ectopic (EAC) within subperiosteal space (in vivo bioreactor), through mere introduction biocompatible gel that might promote hypoxia-mediated chondrogenesis, thereby effectively overcoming aforementioned limitations. The EAC obtained...

A generalized approach to attain spinnability in polymer solutions electrospinning using emulsions is described. By emulsions, low-molecular weight (MW) that tend electrospray can be spun into nanofibers. Additionally, control over fiber properties independent of solution viscosity and MW achieved, with an order magnitude reduction diameter upon emulsification. Nanofibrous sheets obtained demonstrate excellent cellular biocompatibility are suitable for tissue contacting applications....