A microvalve-based bioprinting system for the manufacturing of high-resolution, multimaterial 3D-structures is reported. Applying a straightforward fluid-dynamics model, shear stress at nozzle site can precisely be controlled. Using this system, broad study on how cell viability and proliferation potential are affected by different levels conducted. Complex, 3D structures printed with high resolution. This work pioneers investigation stress-induced damage in might help to comprehend improve...

Over the last decade, bioprinting technologies have begun providing important tissue engineering strategies for regenerative medicine and organ transplantation. The major drawback of past approaches has been poor or inadequate material-printing device substrate combinations, as well relatively small size printed construct. Here, we hypothesise that cell-laden hydrogels can be when submerged in perfluorotributylamine (C12F27N), a hydrophobic high-density fluid, these cells placed within...

The mechanical and physicochemical effects of three-dimensional (3D) printable hydrogels on cell behavior are paramount features to consider before manufacturing functional tissues. We hypothesize that besides good printability cytocompatibility a supporting hydrogel for the manufacture individual tissues, it is equally essential beforehand desired tissue (bone, cartilage, fat). In light its application, structure stiffness matrices influence geometry, which in turn impacts differentiation...

Effective vascularization is crucial for three-dimensional (3D) printed hydrogel-cell constructs to efficiently supply cells with oxygen and nutrients. Till date, several hydrogel blends have been developed that allow the in vitro formation of a capillary-like network within gels but comparatively less effort has made improve suitability materials 3D bioprinting process. Therefore, we hypothesize tailored photo-crosslinkable gelatin type I collagen exhibit favorable drop-on-demand printing...

Recent advances in the field of bioprinting have led to development perfusable complex structures. However, most existing printed vascular channels lack composition or key structural and physiological features natural blood vessels they make use more easily printable but less biocompatible hydrogels. Here, we a drop-on-demand technique generate vitro vessel models, consisting continuous endothelium imitating tunica intima, an elastic smooth muscle cell layer mimicking media, surrounding...

3D-manufactured hydrogels with precise contours and biological adhesion motifs are interesting candidates in the regenerative medicine field for culture differentiation of human bone-marrow-derived mesenchymal stem cells (MSCs). 3D-bioprinting is a powerful technique to approach one step closer native organization cells. This study investigates effect incorporation collagen type I 3D-bioprinted polysaccharide-based modulation cell morphology, osteogenic remodeling potential, mineralization....

In vitro multilayered tissues with mimetic architectures resembling native are valuable tools for application in medical research. this study, an advanced bioprinting strategy is presented aligning collagen fibers contained functional bioinks. Streptavidin-coated iron nanoparticles embedded printable bioinks varying concentrations of low gelling temperature agarose and type I collagen. By applying a straightforward magnetic-based mechanism hydrogels during bioprinting, it possible to align...

Abstract Corneal transplantation is the treatment of choice for patients with advanced corneal diseases. However, outcome may be affected by graft rejection, high associated costs, surgical expertise, and most importantly worldwide donor shortage. In recent years, bioprinting has emerged as an alternative method fabricating tissue equivalents using autologous cells architecture resembling native tissue. this study, we propose a freeform cell‐friendly drop‐on‐demand strategy creating stromal...

Three-dimensional (3D) bioprinting is a promising technology for manufacturing cell-laden tissue-engineered constructs. Larger tissue substitutes, however, require vascularized network to ensure nutrition supply. Therefore, tailored bioinks combining 3D printability and cell-induced vascularization are needed. We hypothesize that hydrogel blends made of agarose-type I collagen agarose-fibrinogen printable will allow the formation capillary-like structures by human umbilical vein endothelial...

In recent years, novel biofabrication technologies have enabled the rapid manufacture of hydrogel-cell suspensions into tissue-imitating constructs. The development materials for still remains a challenge due to gap between contradicting requirements such as three-dimensional printability and optimal cytocompatibility. We hypothesise that blending different hydrogels could lead material with favourable biological printing properties. our work, we combined agarose type I collagen in order...

Abstract This study introduces a thermogelling bioink based on carboxylated agarose (CA) for bioprinting of mechanically defined microenvironments mimicking natural tissues. In CA system, by adjusting the degree carboxylation, elastic modulus printed gels can be tuned over several orders magnitudes (5–230 Pa) while ensuring almost no change to shear viscosity (10–17 mPa) solution; thus enabling fabrication 3D structures made different mechanical domains under identical printing parameters...

The world population is growing and alternative ways of satisfying the increasing demand for meat are being explored, such as using animal cells fabrication cultured meat. Edible biomaterials required supporting structures. Hence, we chose agarose, gellan a xanthan-locust bean gum blend (XLB) support materials with pea soy protein additives analyzed them regarding material properties biocompatibility. We successfully built stable hydrogels containing up to 1% or protein. Higher amounts...

Organs-on-a-Chip (OOCs) have recently led to major discoveries and a better understanding of 3D cell organization, cell-cell interactions tissue response drugs biological cues. However, their complexity variability are still limited by the available fabrication technology. Transparent, cytocompatible high-resolution 3D-printing could overcome these limitations, offering flexible low-cost alternative soft lithography. Many advances been made in stereolithography printing regarding resin...

3D bioprinting possesses the potential to revolutionize contemporary methodologies for fabricating tissue models employed in pharmaceutical research and experimental investigations. This is enhanced by combining with advanced organs-on-a-chip (OOCs), which includes a complex arrangement of multiple cell types representing organ-specific cells, connective tissue, vasculature. However, both OOCs so far demand high degree manual intervention, thereby impeding efficiency inhibiting scalability...

Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according computer-generated three-dimensional model. The lack of mechanical stability printed hinders the fabrication high aspect ratio constructs. Here we present submerged bioprinting, novel technique for freeform liquid fluorocarbon. buoyant density fluorocarbons supports soft by...

The merging of defined nanoscale building blocks with advanced additive manufacturing techniques is eminent importance for the preparation multiscale and highly functional materials de novo designed hierarchical architectures. Here, we demonstrate that hydrogels cellulose nanofibrils (CNF) can be processed into complex shapes, used as a sacrificial template to prepare freestanding cell constructs. We showcase our approach fabrication hollow fibers using controlled extrusion through circular...

In vitro cancer 3D models are valuable tools to provide mechanistic insight into solid tumor growth, invasion, and drug delivery. The spheroid model of tumors has been the most popular in use until now. However, previous studies have shown that these lack sufficient morphological parameters, which may affect their response chemicals. this work, we proposed fabrication miniaturized using collagen type I-based bioprintable bioinks. context a mimicking for advanced neuroblastoma studies, showed...

Human tissues, both in health and disease, are exquisitely organized into complex three-dimensional architectures that inform tissue function. In biomedical research, specifically drug discovery personalized medicine, novel human-based (3D) models needed to provide information with higher predictive value compared state-of-the-art two-dimensional (2D) preclinical models. However, current vitro remain inadequate recapitulate the heterogenous underlie biology. Therefore, it would be beneficial...

Large bone defects are commonly treated by replacement with auto- and allografts, which have substantial drawbacks including limited supply, donor site morbidity, possible tissue rejection. This study aimed to improve defect treatment using a custom-made filament for engineering scaffolds. The consists of biodegradable polylactide acid (PLA) varying amount (up 20%) osteoconductive S53P4 bioglass. By employing an innovative, additive manufacturing technique, scaffolds optimized...

To combat the growing threat of antibiotic resistance, environmental testing for contamination is gaining an increasing role. This study aims to develop easy-to-use assay detection fluoroquinolone levofloxacin. Levofloxacin used in human and veterinary medicine has been detected wastewater river water. An RNA aptamer against levofloxacin was selected using Capture-SELEX. The 73 nt long folds into three stems with a central three-way junction. It binds Kd 6 µM discriminates closely related...

Aim of the study: Deep carious lesions may cause irreversible pulpitis and current endodontic treatment typically removes whole dental pulp tissue, which finally reduces lifespan teeth. Nowadays, most frequent is based on removing infected tissue filling root canal with inert synthetic materials. Tissue engineering approaches are important alternatives to treatment, because they can potentially maintain biological function tooth instead sacrificing it.Materials Methods: In this study, we...

In Bone Tissue Engineering (BTE), autologous bone-regenerative cells are combined with a scaffold for large bone defect treatment (LBDT). Microporous, polylactic acid (PLA) scaffolds showed good healing results in small animals. However, transfer to animal models is not easily achieved simply by upscaling the design. Increasing diffusion distances have negative impact on cell survival and nutrition supply, leading death ultimately implant failure. Here, novel architecture was designed meet...