A5005025349- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Intraocular Surgery and Lenses
- Ocular Infections and Treatments
- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Ophthalmology and Visual Impairment Studies
- Retinal Imaging and Analysis
- Ocular Disorders and Treatments
- Proteoglycans and glycosaminoglycans research
- Retinal and Optic Conditions
- Ophthalmology and Eye Disorders
- Endoplasmic Reticulum Stress and Disease
- Heart Rate Variability and Autonomic Control
- Intraoperative Neuromonitoring and Anesthetic Effects
- Retinal Development and Disorders
- Periodontal Regeneration and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Visual perception and processing mechanisms
- Diabetes and associated disorders
- Allergic Rhinitis and Sensitization
- CRISPR and Genetic Engineering
- Autophagy in Disease and Therapy
RWTH Aachen University
2016-2025
Universitätsklinikum Aachen
2013-2025
Ophthalmology Clinic
2015-2025
Singapore Eye Research Institute
2016-2019
Committee on Publication Ethics
2017-2018
Singapore National Eye Center
2017
Duke-NUS Medical School
2017
Nanyang Technological University
2017
Christie's
2016
Universitätsklinik für Augenheilkunde
2015
Abstract Corneal transplantation is the treatment of choice for patients with advanced corneal diseases. However, outcome may be affected by graft rejection, high associated costs, surgical expertise, and most importantly worldwide donor shortage. In recent years, bioprinting has emerged as an alternative method fabricating tissue equivalents using autologous cells architecture resembling native tissue. this study, we propose a freeform cell‐friendly drop‐on‐demand strategy creating stromal...
Corneal endothelial dysfunction remains the most frequent indication for corneal transplantation, limited by donor material shortage, poor long-term graft survival, or allogeneic rejection. Therefore, tissue-engineered grafts (TEEG) represent a promising alternative to human tissue. In this study, we generated electro-spun scaffolds and tested these their suitability cell (hCEC) cultivation.The polymers poly(methyl-methacrylate) (PMMA), poly(lactic-co-glycolic acid) (PLGA), polycaprolactone...
Abstract Endoplasmic reticulum (ER) stress due to accumulation of hepatoviral or misfolded proteins is increasingly recognized as an important step in the pathogenesis inflammatory, toxic, and metabolic liver diseases. ER results activation several intracellular signaling pathways including Jun N-terminal kinase (JNK). The AP-1 (activating protein 1) transcription factor c-Jun a prototypic JNK target regulator hepatocyte survival, proliferation, tumorigenesis. Because functions during...
Abstract Laser refractive surgeries reshape corneal stroma to correct errors, but unavoidably affect nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization neuro-epitheliopathy. Following injury, surviving stromal keratocytes (CSKs) are activated fibroblasts (SFs). How these two different cell types influence is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from same using an in vitro chick dorsal root ganglion...
To compare the efficacy and safety of combined phacoemulsification with ab interno canaloplasty (Phaco-AbiC) versus single-use dual blade goniotomy (Phaco-DBG). In this prospective consecutive case series, intraocular pressure (IOP), number IOP-lowering medications, mean deviation (MD) visual field testing, surgery-related complications were evaluated in 25 eyes following Phaco-AbiC 20 Phaco-DBG through 12 months follow-up. Qualified or complete success was defined as postoperative IOP lower...
Purpose: To report the long-term recurrence and complication rates after femtosecond laser–assisted pterygium excision surgery (FLAPS) with conjunctival autograft surgery. Methods: Single-center retrospective study of consecutive cases primary nasal and/or temporal pterygia who underwent autograft. All subjects manual excision, followed by laser (Femto LDV Z8)-assisted follow-up duration up to 5 years. Recurrence was defined as appearance secondary at same location. complications intra-...
Purpose: To evaluate the safety and feasibility of intrastromal injection human corneal stromal keratocytes (CSKs) its therapeutic effect on a rodent early opacity model. Methods: Twelve research-grade donor corneas were used in primary culture to generate quiescent CSKs activated fibroblasts (SFs). Single repeated injections 2 4 × 104 cells rat normal (n = 52) or with opacities induced by irregular phototherapeutic keratectomy 16) performed, followed weekly examination response under...
Abstract Keratoconus (KC) is an ectatic corneal disease characterized by progressive thinning and irregular astigmatism, a leading indication for transplantation. KC-associated changes have been demonstrated the entire cornea, but pathological mechanical weakening usually localized. We performed quantitative proteomics using Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectrometry (SWATH-MS) to analyze epithelial stromal between topographically-abnormal cone...
We evaluated the small molecules (AFM) caffeine, curcumin and pirfenidone to find non-toxic concentrations reducing transformation of activated human corneal stromal keratocytes (aCSK) scar-inducing myofibroblasts (MYO-SF). CSK were isolated from 16 corneas unsuitable for transplantation expanded three passages in control medium (0.5% FBS). Then, aCSK exposed caffeine 0−500 μM, 0−200 0−2.2 nM profibrotic cytokine TGF-β1 (10 ng/mL) 48 h. Alterations viability gene expression by cell staining...
Abstract Corneal opacities are a leading cause of global blindness. They conventionally treated by the transplantation donor corneal tissue, which is, restricted worldwide material shortage and allograft rejection. Autologous adult stem cells with potential to differentiate into stromal keratocytes ( CSK s) could offer suitable choice for regenerative cell therapy. Postnatal periodontal ligament PDL ) contains population cells, has similar embryological origin as , that is cranial neural...
Purpose: Pterygium is a prevalent ocular surface disorder. Conjunctival autografting (CAG) after pterygium resection the gold standard treatment. Thinner CAGs without Tenon tissue provide better results but are technically difficult to achieve. We report on first femtosecond laser (FSL)-assisted surgery (FLAPS) in humans. Methods: In clinical trial, we treated 6 eyes of 5 patients with FLAPS. After manual removal pterygium, were dissected Ziemer Z8 at 60-μm depth and glued into defect. CAG...
To analyse structural changes in conjunctiva, sclera and Schlemm's canal (SC) following canaloplasty with optical coherence tomography (AS-OCT) ultrasound biomicroscopy (UBM).Fifteen patients undergoing were included this prospective study. AS-OCT images acquired pre- 1, 7, 30 90 days postoperatively. UBM was performed 3 months The surgical site evaluated for the presence of SC, transscleral filtration, a scleral lake visibility intra-Schlemm-sutures. height width SC measured at 9 o'clock...
Abstract Purpose Previous reports showed increased flow velocities in retrobulbar vessels after glaucoma surgery the first weeks. Colour Doppler imaging was performed to investigate long‐term effects of trabeculectomy on haemodynamics patients with primary open–angle ( POAG ). Methods In a prospective study 30 (mean age 63.2 ± 15.4 years) were included. before 1–2 weeks, 2 months, 4–6 and up 3 years determine peak systolic end–diastolic ophthalmic artery, central retinal short nasal temporal...