A5041900450- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Ultrasound and Hyperthermia Applications
- Electrospun Nanofibers in Biomedical Applications
- Advanced Materials and Mechanics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Photoacoustic and Ultrasonic Imaging
- Microfluidic and Capillary Electrophoresis Applications
- Nanoplatforms for cancer theranostics
- Ultrasound and Cavitation Phenomena
- Pickering emulsions and particle stabilization
- Ultrasound Imaging and Elastography
- Sirtuins and Resveratrol in Medicine
- Clostridium difficile and Clostridium perfringens research
- Microfluidic and Bio-sensing Technologies
- Angiogenesis and VEGF in Cancer
- Advanced Sensor and Energy Harvesting Materials
- Luminescence and Fluorescent Materials
- Blood properties and coagulation
- Escherichia coli research studies
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Tissue Engineering and Regenerative Medicine
- Photonic Crystals and Applications
- Electrohydrodynamics and Fluid Dynamics
DWI – Leibniz Institute for Interactive Materials
2017-2024
RWTH Aachen University
2017-2024
Westfälische Hochschule
2023
FH Aachen
2018
Recent advances in the field of bioprinting have led to development perfusable complex structures. However, most existing printed vascular channels lack composition or key structural and physiological features natural blood vessels they make use more easily printable but less biocompatible hydrogels. Here, we a drop-on-demand technique generate vitro vessel models, consisting continuous endothelium imitating tunica intima, an elastic smooth muscle cell layer mimicking media, surrounding...
In this work, a two component microgel assembly using soft anisometric microgels that interlink to create 3D macroporous construct for cell growth is reported. Reactive rods with variable aspect ratio are produced via microfluidics in continuous plug-flow on-chip gelation method by photoinitiated free-radical polymerization of star-polyethylene glycol-acrylate glycidyl methacrylate or 2-aminoethyl comonomers. The resulting complementary epoxy- and amine-functionalized assemble each other...
Abstract Growing millimeter‐scaled functional tissue remains a major challenge in the field of engineering. Therefore, microporous annealed particles (MAPs) are emerging as promising porous biomaterials that formed by assembly microgel building blocks. To further vary pore size and increase overall MAP porosity mechanically stable scaffolds, rod‐shaped microgels with high aspect ratios up to 20 chemically interlinked into highly scaffolds. Polyethylene glycol based (width 10 µm, lengths 200...
Surface tension provides microbubbles (MB) with a perfect spherical shape. Here, we demonstrate that MB can be engineered to nonspherical, endowing them unique features for biomedical applications. Anisotropic were generated via one-dimensionally stretching poly(butyl cyanoacrylate) above their glass transition temperature. Compared counterparts, nonspherical polymeric displayed superior performance in multiple ways, including i) increased margination behavior blood vessel-like flow...
In the past decade, anisometric rod-shaped microgels have attracted growing interest in materials-design and tissue-engineering communities. Rod-shaped exhibit outstanding potential as versatile building blocks for 3D hydrogels, where they introduce macroscopic anisometry, porosity, or functionality structural guidance biomaterials. Various fabrication methods been established to produce such shape-controlled elements. However, continuous high-throughput production of with simultaneous...
Human induced pluripotent stem cells (hiPSCs) are used as an alternative for human embryonic cells. Cardiomyocytes derived from hiPSCs employed in cardiac tissue regeneration constructs due to the heart's low capacity after infarction. A coculture of hiPSC-CM and primary dermal fibroblasts is encapsulated injectable poly(ethylene glycol)-based microgels via microfluidics enhance efficiency regenerative cell transplantations. The prepared Michael-type addition multi-arm PEG-based molecules...
Background: Docetaxel and doxorubicin combination has been widely used in anaplastic thyroid cancer/ATC treatment but often results serious adverse effects drug resistance.Resveratrol effectively inhibits ATC cell proliferation vitro without affecting the corresponding normal cells, while its vivo anti-ATC especially on ones with docetaxel/doxorubicin-resistance have not reported due to low bioavailability.Nanoparticles sustained-release cancer-targeting features may overcome this...
We report coupling of a photoacid cycle to colloidal assembly leading dissipative disassembly reaction network.
Microbubbles (MB) are routinely used ultrasound (US) contrast agents that have recently attracted increasing attention as stimuli-responsive drug delivery systems. To better understand MB-based delivery, we studied the role of hydrophobicity and molecular weight on MB loading, shelf-life stability, US properties, release. Eight model drugs, varying in weight, were loaded into shell poly(butyl cyanoacrylate) (PBCA) MB. In case drugs with progesterone a common structural backbone (i.e., for...
Poly(n-butyl cyanoacrylate) microbubbles (PBCA-MB) are extensively employed for functional and molecular ultrasound (US) imaging, as well US-mediated drug delivery. To facilitate the use of PBCA-MB a commercial platform biomedical applications, it is important to systematically study improve their stability shelf-life. In this context, lyophilization (freeze drying) widely used increase shelf-life promote product development. Here, we set out analyze standard rhodamine-B loaded at three...
Soft amphoteric microgel systems exhibit a rich phase behavior. Crystalline phases of these material are interest because they photonic stop-gaps, giving rise to iridescent color. Such promising for applications in soft, switchable, and programmable filters devices. We here report composite system consisting hard fluorescently labeled core shell. At pH above the isoelectric point (IEP), colloids easily crystallize into three-dimensional colloidal assemblies. By adding cyclic lactone system,...
The filtration performance of soft colloid suspensions suffers from the agglomeration colloids on membrane surface as filter cakes. Backflushing fluid through and cross-flow flushing across are widely used methods to temporally remove cake restore flux membrane. However, phenomena occurring during recovery not yet fully described. In this study, we filtrate poly(N-isopropylacrylamide) microgels analyze in terms its composition dynamic mobility removal using on-line laser scanning confocal...
Vibrio cholerae is a Gram-negative bacterium that causes secretory diarrhea and constitutes major health threat in the industrialized world even more developing countries. Its main virulence factor cholera toxin, which internalized by intestinal epithelial cells after binding to glycosphingolipid receptor GM1a on their apical surface. A potential future solution dampen complications of infection scavenging toxin presenting competitive motifs diminish vivo toxicity V. cholerae. Here, we...
Microgels are water-swollen, crosslinked polymers that widely used as colloidal building blocks in scaffold materials for tissue engineering and regenerative medicine. can be controlled their stiffness, degree of swelling, mesh size depending on polymer architecture, crosslink density, fabrication method-all which influence function interaction with the environment. Currently, there is a lack understanding how composition influences internal structure soft microgels this morphology affects...
Therapeutic antibodies are the key treatment option for various cytokine-mediated diseases, such as rheumatoid arthritis, psoriasis, and inflammatory bowel disease. However, systemic injection of these can cause side effects suppress immune system. Moreover, clearance therapeutic from blood is limiting their efficacy. Here, water-swollen microgels produced with a size 25 µm using droplet-based microfluidics. The functionalized TNFα to locally scavenge pro-inflammatory cytokine TNFα....
Abstract Although micron‐sized microgels have become important building blocks in regenerative materials, offering decisive interactions with living matter, their chemical composition mostly significantly varies when network morphology is tuned. Since cell behavior simultaneously affected by the physical, chemical, and structural properties of gel network, variable but equivalence are interest. This work describes a new method to produce thermoresponsive defined mechanical properties,...
Abstract Although micron‐sized microgels have become important building blocks in regenerative materials, offering decisive interactions with living matter, their chemical composition mostly significantly varies when network morphology is tuned. Since cell behavior simultaneously affected by the physical, chemical, and structural properties of gel network, variable but equivalence are interest. This work describes a new method to produce thermoresponsive defined mechanical properties,...
A broad spectrum ROS/RNS sensing butylated phenol tethered leucomethylene blue is presented. This probe detects a variety of ROS/RNS, with up to 100-fold gain in fluorescence the red range, suitable for microscopic and macroscopic vivo imaging.
Rod-Shaped Microgels A two-component system consisting of microgel rods creates macroporous interlinked constructs for cell culture in 3D with pores up to several hundreds micrometers. The functionalized that interlink each other aqueous solutions without further additives are produced via microfluidics a continuous plug-flow on-chip gelation method. More details can be found article number 2103554 by Laura De Laporte and co-workers.
A two-component system of functionalized microgels from microfluidics allows for fast interlinking into 3D macroporous constructs in aqueous solutions without further additives. Continuous photoinitiated on-chip gelation enables variation the microgel aspect ratio, which determines building block properties obtained constructs. Glycidyl methacrylate (GMA) or 2-aminoethyl (AMA) monomers are copolymerized network based on polyethylene glycol (PEG) star-polymers to achieve either epoxy amine...
Abstract Microgels are water‐swollen, crosslinked polymers that widely used as colloidal building blocks in scaffold materials for tissue engineering and regenerative medicine. can be controlled their stiffness, degree of swelling, mesh size depending on polymer architecture, crosslink density, fabrication method—all which influence function interaction with the environment. Currently, there is a lack understanding how composition influences internal structure soft microgels this morphology...
A two-component system of functionalized microgels from microfluidics allows for fast interlinking into 3D macroporous constructs in aqueous solutions without further additives. Continuous photoinitiated on-chip gelation enables variation the microgel aspect ratio, which determines building block properties obtained constructs. Glycidyl methacrylate (GMA) or 2-aminoethyl (AMA) monomers are copolymerized network based on polyethylene glycol (PEG) star-polymers to achieve either epoxy amine...