A5100640634- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Cavitation Phenomena
- Ultrasound Imaging and Elastography
- Vagus Nerve Stimulation Research
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Alzheimer's disease research and treatments
- Cell Adhesion Molecules Research
- Anesthesia and Pain Management
- Power Systems and Technologies
- Monoclonal and Polyclonal Antibodies Research
- Cerebrospinal fluid and hydrocephalus
- Multiple Sclerosis Research Studies
- Advanced biosensing and bioanalysis techniques
- Hedgehog Signaling Pathway Studies
- RNA Interference and Gene Delivery
- Neuroscience and Neural Engineering
- Extracellular vesicles in disease
- Pancreatic function and diabetes
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Hemispheric Asymmetry in Neuroscience
- Salivary Gland Disorders and Functions
Harvard University
2006-2024
Stanford University
2024
Beijing Institute of Technology
2024
Brigham and Women's Hospital
2002-2023
Nanjing University
2012-2023
Chengdu University of Traditional Chinese Medicine
2023
Nanjing Medical University
2023
Qilu Hospital of Shandong University
2023
Nanjing General Hospital of Nanjing Military Command
2023
Tufts University
2017-2022
The human left and right cerebral hemispheres are anatomically functionally asymmetric. To test whether cortical asymmetry has a molecular basis, we studied gene expression levels between the embryonic using serial analysis of (SAGE). We identified verified 27 differentially expressed genes, which suggests that is accompanied by early, marked transcriptional asymmetries. LMO4 consistently more highly in perisylvian cortex than essential for development mice, suggesting left-right...
Cavitation-facilitated microbubble-mediated focused ultrasound therapy is a promising method of drug delivery across the blood-brain barrier (BBB) for treating many neurological disorders. Unlike thermal therapies, during which magnetic resonance thermometry can serve as reliable treatment control modality, real-time modulated BBB disruption with undetectable vascular damage remains challenge. Here closed-loop cavitation controlling paradigm that sustains stable while suppressing inertial...
The molecular mechanisms by which Notch receptors induce diverse biological responses are not fully understood. We recently cloned a mammalian homologue of the Mastermind gene Drosophila melanogaster, MAML1 (Mastermind-like-1 molecule) and determined that it functions as transcriptional coactivator for receptors. In this report, we characterize two additional genes in Mastermind-like family: MAML2 MAML3. three MAML widely expressed adult tissues but exhibit distinct expression patterns mouse...
Cavitation events seeded by microbubbles have been previously reported to be associated with MR- or fluorescent-contrast enhancement after focused ultrasound (FUS)-induced blood-brain barrier (BBB) opening. However, it is still unknown whether bubble activity can correlated the reversibility (the duration of opening and likelihood safe reinstatement) permeability opened BBB, which critical for clinical translation using passive cavitation detection monitor, predict control In this study,...
The blood-brain barrier (BBB) restricts delivery of most chemotherapy agents to brain tumors.Here, we investigated a clinical focused ultrasound (FUS) device disrupt the BBB in rats and enhance carboplatin using F98 glioma model.Methods: In each rat, 2-3 volumetric sonications (5 ms bursts at 1.1 Hz for 75s) targeted 18-27 locations one hemisphere.Sonication was combined with Definity microbubbles (10 µl/kg) followed by intravenous (50 mg/kg).Closed-loop feedback control performed based on...
Abstract Approaches to safely and effectively augment cellular functions without compromising the inherent biological properties of cells, especially through integration biologically labile domains, remain great interest. Here, a versatile strategy assemble active nanocomplexes, including proteins, DNA, mRNA, even viral carriers, on surfaces generate cell‐based hybrid system referred as “Cellnex” is established. This can be used engineer wide range cell types in adoptive transfers,...
The choroid plexus (ChP) of the brain plays a central role in orchestrating recruitment peripheral leukocytes into nervous system (CNS) through blood-cerebrospinal fluid (BCSF) barrier pathological conditions, thus offering unique niche to diagnose CNS disorders. We explored whether magnetic resonance imaging ChP could be optimized for mild traumatic injury (mTBI). mTBI induces subtle, yet influential, changes and is currently severely underdiagnosed. hypothesized that sufficient alterations...
Surface tension provides microbubbles (MB) with a perfect spherical shape. Here, we demonstrate that MB can be engineered to nonspherical, endowing them unique features for biomedical applications. Anisotropic were generated via one-dimensionally stretching poly(butyl cyanoacrylate) above their glass transition temperature. Compared counterparts, nonspherical polymeric displayed superior performance in multiple ways, including i) increased margination behavior blood vessel-like flow...
Central nervous system (CNS) diseases are difficult to treat because of the blood-brain barrier (BBB), which prevents most drugs from entering into brain. Intranasal (IN) administration is a promising approach for drug delivery brain, bypassing BBB; however, its application has been restricted particularly potent substances and it does not offer localized specific brain sites. Focused ultrasound (FUS) in combination with microbubbles can deliver at targeted locations. The present study...
Microbubbles (MB) are widely used for ultrasound (US) imaging and drug delivery. MB typically spherically shaped, due to surface tension. When heated above their glass transition temperature, polymer-based can be mechanically stretched obtain an anisotropic shape, endowing them with unique features US-mediated blood-brain barrier (BBB) permeation. It is here shown that nonspherical surface-modified BBB-specific targeting ligands, thereby promoting binding sonopermeation of blood vessels in...
Abstract We investigated controlled blood-brain barrier (BBB) disruption using a low-frequency clinical transcranial MRI-guided focused ultrasound (TcMRgFUS) device and evaluated enhanced delivery of irinotecan chemotherapy to the brain rat glioma model. Animals received three weekly sessions FUS, FUS 10 mg/kg irinotecan, or alone. In each session, four volumetric sonications targeted 36 locations in one hemisphere. With feedback control based on recordings acoustic emissions, 98% sonication...
In developing neural tube, the basic helix-loop-helix (bHLH) transcription factor Olig2 interacts with homeodomain Nkx2.2 at two distinct stages. During neuronogenesis, a cross-repressive interaction appears to establish precise boundary between p3 and pMN domains. At later times, cooperative is noted because promotes maturation of oligodendrocyte progenitor cells specified by expression Olig2. We show here that protein can form physical complex in mammalian yeast two-hybrid trap assay. This...
The delivery of most therapeutic agents is rendered ineffective for the treatment brain diseases due to presence blood-brain barrier (BBB). goal this study was investigate effect pre-infusion focused ultrasound (FUS) and microbubbles on distribution direct infusion in vivo. A single-element FUS transducer used all sonications, which were carried out immediately prior procedures. Mice received either Gadolinium-labeled albumin (Gd-albumin, 74 kDa) or adeno-associated virus (AAV, ∼4 MDa)....
The therapeutic potential of many gene therapies is limited by their inability to cross the blood brain barrier (BBB). While intranasal administration plasmid DNA nanoparticles (NPs) offers a non-invasive approach bypass BBB, it not targeted disease-relevant regions. Here, our goal was determine whether focused ultrasound (FUS) can enrich delivery NPs target deeper regions, in this case regions most affected Parkinson's disease. Combining FUS with resulted enhanced rodent brain, recruitment...
Abstract Ca 2+ oscillation is a system-level property of the cellular -handling machinery and encodes diverse physiological pathological signals. The present study tests hypothesis that oscillations play vital role in maintaining stemness liver cancer stem cells (CSCs), which are postulated to be responsible for initiation progression. We found niche factor-stimulated signature feature CSC-enriched Hep-12 purified α2δ1 + CSC fractions from hepatocellular carcinoma cell lines. In cells,...
Colorectal cancer, common in both men and women, occurs when tumors form the linings of colon. Common treatments colorectal cancer include surgery, chemotherapy, radiation therapy; however, many often damage healthy tissues cells, inducing severe side effects. Conventional chemotherapeutic agents such as doxorubicin (Dox) can be potentially used for treatment cancer; they suffer from limited targeting lack selectivity. Here, we report that complexed to hyaluronic acid (HA) (HA-Dox) exhibits...
Abstract Effective chemotherapy delivery for glioblastoma multiforme (GBM) is limited by drug transport across the blood–brain barrier and poor efficacy of single agents. Polymer–drug conjugates can be used to deliver combinations with a ratiometric dosing. However, behaviors effectiveness this system have never been well investigated in GBM models. Here, we report flexible hyaluronic acid (HA) camptothecin (CPT) doxorubicin (DOX) delivered into brain using focused ultrasound (FUS). In vitro...
Sub-harmonic response from ultrasound contrast agent microbubbles has been demonstrated to be an effective modality for noninvasive pressure measurement. In the present study, dependence of ultra-harmonic on ambient overpressure was investigated by both experimental measurements and simulations. measurements, were exposed Gaussian pulses with varied driving frequencies pulse lengths, at acoustic 0.3 MPa. The amplitudes sub- components measured when overpressures 0–25 kPa. At frequency 1.33...
Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid plaques and hyperphosphorylated tau in brain. Currently, therapeutic agents targeting appear promising for AD, however, delivery to CNS limited due blood-brain-barrier (BBB). Focused ultrasound (FUS) method induce temporary opening BBB enhance CNS. In this study, we evaluated acute effects FUS whether use FUS-induced enhances 07/2a mAb, an anti-pyroglutamate-3 Aβ antibody, aged 24 mo-old...