A5050034196- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound Imaging and Elastography
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- Ultrasound and Cavitation Phenomena
- Tissue Engineering and Regenerative Medicine
- Cancer, Stress, Anesthesia, and Immune Response
- Luminescence and Fluorescent Materials
- Liver Disease Diagnosis and Treatment
- Barrier Structure and Function Studies
- Medical Imaging Techniques and Applications
- Veterinary Pharmacology and Anesthesia
- Cancer, Hypoxia, and Metabolism
- Anesthesia and Neurotoxicity Research
- Radiopharmaceutical Chemistry and Applications
- Erythropoietin and Anemia Treatment
- S100 Proteins and Annexins
- Effects of Radiation Exposure
- Drug-Induced Hepatotoxicity and Protection
- Cancer Immunotherapy and Biomarkers
- Thermoregulation and physiological responses
- Liver physiology and pathology
RWTH Aachen University
2016-2025
Universitätsklinikum Aachen
2021-2025
Maastricht University
2017
University of Medicine and Pharmacy of Craiova
2017
University of Twente
2017
German Centre for Cardiovascular Research
2017
Institutul National Victor Babes
2017
Faculty (United Kingdom)
2015
FH Aachen
2015
Guy's Hospital
1974
Abstract Super-resolution imaging methods promote tissue characterization beyond the spatial resolution limits of devices and bridge gap between histopathological analysis non-invasive imaging. Here, we introduce motion model ultrasound localization microscopy (mULM) as an easily applicable robust new tool to morphologically functionally characterize fine vascular networks in tumors at super-resolution. In tumor-bearing mice for first time patients, demonstrate that within less than 1 min...
Conjugated polymer nanoparticles exhibit strong fluorescence and have been applied for biological imaging in cell culture small animals. However, conjugated particles are hydrophobic often chemically inert materials with diameters ranging from below 50 nm to several microns. As such, cannot be excreted through the renal system. This drawback has prevented their application clinical bio-medical imaging. Here, we present fully based on imidazole units. These can bio-degraded by activated...
Immune checkpoint inhibitors have shown clinical benefit in several cancer entities including metastatic microsatellite instable colorectal carcinomas. However, for the majority of carcinomas potential and limitations immune inhibition is not fully understood. In this study, effects sole dual CTLA-4 PD-L1 blockade were investigated a stable highly aggressive orthotopic mouse model colon cancer. Dual resulted tumor growth stagnation completely blocked liver metastasis. Sole only moderately...
Patients with advanced colorectal cancer (CRC) have a poor prognosis. Combinations of immunotherapies and anti-angiogenic agents are currently being evaluated in clinical trials. In this study, the multikinase inhibitor regorafenib (REG) was combined an anti-programmed cell death protein 1 (aPD1) antibody syngeneic murine microsatellite-stable (MSS) CT26 hypermutated MC38 colon models to gain mechanistic insights into potential drug synergism.Growth progression orthotopic subcutaneous...
Riboflavin transporters (RFTs) and the riboflavin carrier protein (RCP) are highly upregulated in many tumor cells, stem neovasculature, which makes them attractive targets for nanomedicines. Addressing cells different compartments requires drug carriers, not only able to accumulate via EPR effect but also extravasate, target specific cell populations, get internalized by cells. Reasoning that antibodies among most efficient targeting systems developed nature, we consider their size (∼10–15...
Abstract Photoacoustics is a powerful biomedical imaging and detection technique, because it noninvasive, nonionizing, low‐cost method facilitating deep tissue penetration. However, suitable contrast agents need to be developed increase the for in vivo imaging. Gold nanoparticles are often discussed as potential sonophores due their large absorption cross‐section tunable plasmon resonance. disadvantages such toxicity low photoacoustic efficiency transparency window prevail, preventing...
Ultrasound is among the most rapidly advancing imaging techniques. Functional methods such as elastography have been clinically introduced, and tissue characterization improved by contrast-enhanced scans. Here, novel superresolution techniques provide unique morphologic functional insights into vascularization. analyses are complemented molecular ultrasound imaging, to visualize markers of inflammation angiogenesis. The full potential diagnostic may become apparent integrating these multiple...
Photoacoustics is an upcoming modality for biomedical imaging, which promises minimal invasiveness at high penetration depths of several centimeters. For superior photoacoustic contrast, imaging probes with photothermal conversion efficiency are required. Gold nanoparticles among the best performing probes. However, geometry and size determine their efficiency. We present a systematic theoretical analysis to optimum nanoparticle respect in near-infrared spectral range, contrast. Theoretical...
Biohybrid tissue-engineered vascular grafts (TEVGs) promise long-term durability due to their ability adapt hosts' needs. However, the latter calls for sensitive non-invasive imaging approaches longitudinally monitor functionality, integrity, and positioning. Here, we present an approach comprising labeling of non-degradable degradable TEVGs' components in vitro vivo monitoring by hybrid
The process of locally increasing the permeability cell membranes or layers is referred to as sonoporation sonopermeation, respectively, and opens up perspectives for drug delivery in cancer treatment by facilitating enhanced local accumulation. These effects are mediated ultrasound-activated microbubbles close proximity cells. Here, selection ultrasound settings according intended effect on biological tissue remains a challenge, especially broadly size-distributed microbubbles, which show...
Background: Docetaxel and doxorubicin combination has been widely used in anaplastic thyroid cancer/ATC treatment but often results serious adverse effects drug resistance.Resveratrol effectively inhibits ATC cell proliferation vitro without affecting the corresponding normal cells, while its vivo anti-ATC especially on ones with docetaxel/doxorubicin-resistance have not reported due to low bioavailability.Nanoparticles sustained-release cancer-targeting features may overcome this...
Preclinical and clinical data indicate that contrast-enhanced ultrasound can enhance tumor perfusion vessel permeability, thus, improving chemotherapy accumulation therapeutic outcome. Therefore, we investigated the effects of high mechanical index (MI) Doppler (CDUS) on in breast cancer.
Tissue-engineered vascular grafts (TEVGs) with the ability to grow and remodel open new perspectives for cardiovascular surgery. Equipping TEVGs synthetic polymers biological components provides a good compromise between high structural stability adaptability. However, imaging approaches control grafts' integrity, physiological function, remodeling during entire transition late in vitro maturation early vivo engraftment are mandatory clinical implementation. Thus, comprehensive molecular...
The purposes of this study were the development and preclinical evaluation clinically translatable E-selectin-specific ultrasound contrast agents based on a peptide ligand with recognition sequence IELLQAR.The was synthesized through solid phase synthesis covalently attached to poly n-butylcyanoacrylate-stabilized microbubbles an air core. Quantification microbubble surface coverage peptides performed flow cytometry. Targeted adhesion peptide-coated investigated in vitro using parallel plate...
Here, we present a facile one-step Sonogashira dispersion polymerization affording monodisperse conjugated polymer particles bearing accessible acetylene moieties on the surface. These groups are easily functionalized with biological recognition motifs using thiol-yne click chemistry. The resulting functional applied as fluorescent probes for imaging of activated endothelial cells, which take up via receptor mediated endocytosis.
The junctional adhesion molecule A (JAM-A) is physiologically located in interendothelial tight junctions and focally redistributes to the luminal surface of blood vessels under abnormal shear flow conditions accompanying atherosclerotic lesion development. Therefore, JAM-A was evaluated as a target for molecularly targeted ultrasound imaging transient endothelial dysfunction acute variations.Flow-dependent induced apolipoprotein E-deficient mice (n=43) by carotid partial ligation....
Score sheets were first introduced 30 years ago to assess pain, distress and suffering in animals. To date, however, there is still no general agreement on their use research practice, only a few publications can be found this topic. In the present work, we demonstrate of special score sheet for severity assessment three postoperative days two showcased studies performed Wistar Lewis rats undergoing liver resection or orthotopic transplantation, respectively. Scoring different criteria total...
Abstract Despite its high potential, non‐viral gene therapy of cancer remains challenging due to inefficient nucleic acid delivery. Ultrasound (US) with microbubbles (MB) can open biological barriers and thus improve DNA mRNA passage. Polymeric MB are an interesting alternative clinically used lipid‐coated because their stability, narrow size distribution, easy functionalization. However, besides choosing the ideal MB, it unclear whether nanocarrier‐encapsulated should be administered...