A5006443870- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Nanocluster Synthesis and Applications
- Click Chemistry and Applications
- Quantum Dots Synthesis And Properties
- Advanced Biosensing Techniques and Applications
- Chalcogenide Semiconductor Thin Films
- Monoclonal and Polyclonal Antibodies Research
- Supercapacitor Materials and Fabrication
- Mycotoxins in Agriculture and Food
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Photoacoustic and Ultrasonic Imaging
- Photochromic and Fluorescence Chemistry
- Immune cells in cancer
- Molecular Junctions and Nanostructures
- Biosensors and Analytical Detection
- Protein purification and stability
- Graphene research and applications
- Advancements in Battery Materials
University of Massachusetts Amherst
2017-2024
University of Havana
2014-2020
Rede de Química e Tecnologia
2019
Amherst College
2017
Bioorthogonal catalysis offers a unique strategy to modulate biological processes through the in situ generation of therapeutic agents. However, direct application bioorthogonal transition metal catalysts (TMCs) complex media poses numerous challenges due issues limited biocompatibility, poor water solubility, and catalyst deactivation environments. We report here creation catalytic "polyzymes", comprised self-assembled polymer nanoparticles engineered encapsulate lipophilic TMCs. The...
Bioorthogonal catalysis using transition-metal catalysts (TMCs) provides a toolkit for the in situ generation of imaging and therapeutic agents biological environments. Integrating TMCs with nanomaterials mimics key properties natural enzymes, providing bioorthogonal "nanozymes". ZnS nanoparticles provide platform nanozymes ruthenium embedded self-assembled monolayers on particle surface. These uncage allylated profluorophores prodrugs. The core combines non-toxicity degradability...
Polarization of macrophage to M1 phenotype by in situ activation imiquimod using bioorthogonal nanozymes for anticancer therapy.
Abstract Photoacoustics is a powerful biomedical imaging and detection technique, because it noninvasive, nonionizing, low‐cost method facilitating deep tissue penetration. However, suitable contrast agents need to be developed increase the for in vivo imaging. Gold nanoparticles are often discussed as potential sonophores due their large absorption cross‐section tunable plasmon resonance. disadvantages such toxicity low photoacoustic efficiency transparency window prevail, preventing...
Bioorthogonal transformation of prodrugs and profluorophores using transition metal catalysts (TMCs) offers a promising strategy for therapeutic imaging applications. Here, we report the surface engineering nanoparticles to specifically localize gold (AuNPs) with encapsulated TMCs (nanozymes) either inside or outside cells. The ability control nanozyme localization hence activity was demonstrated by activation pro-fluorophores intra- extracellularly, establishing potential engineered...
Herein, a novel drug photorelease system based on gold nanostars (AuNSts), coated with mesoporous silica shell and capped paraffin as thermosensitive molecular gate, is reported. Direct measurements of the surface temperature single nanostar irradiated using tightly focused laser beam are performed via heat-sensitive biological matrix. The AuNSt increases by hundreds degrees (°C) even at low powers. AuNSts surfactant-templated synthesis used chemotherapeutic nanocarriers. Synthetic...
Macrophage-targeted bioorthogonal nanozyme designed to generate antibiotics at the site of infection. Nanozymes had targeted uptake by macrophages through mannose functionalization, then generated specifically kill infectious bacteria.
Janus gold nanostar-mesoporous silica nanoparticle (AuNSt-MSNP) nanodevices able to release an entrapped payload upon irradiation with near infrared (NIR) light were prepared and characterized. The AuNSt surface was functionalized a thiolated photolabile molecule (5), whereas the mesoporous face loaded model drug (doxorubicin) capped proton-responsive benzimidazole-β-cyclodextrin supramolecular gatekeepers (N 1). Upon NIR-light, compound 5 photodissociated, resulting in formation of succinic...
Abstract Bioorthogonal catalysis provides a promising strategy for imaging and therapeutic applications, providing controlled in situ activation of pro‐dyes prodrugs. In this work, the use polymeric scaffold to encapsulate transition metal catalysts (TMCs), generating bioorthogonal “polyzymes,” is presented. These polyzymes enhance stability TMCs, protecting catalytic centers from deactivation biological media. The potential these demonstrated by transformation nontoxic prodrug an anticancer...
Surface functional groups of nanozyme ligands dictate their kinetic behavior.
Gold nanostars coated with a mesoporous silica shell and functionalised PEG containing photolabile 2-nitrobenzyl moieties released doxorubicin after NIR light irradiation.
Control over supramolecular recognition between proteins and nanoparticles (NPs) is of fundamental importance in therapeutic applications sensor development. Most NP-protein binding approaches use 'tags' such as biotin or His-tags to provide high affinity; protein surface provides a versatile alternative strategy. Generating affinity interactions challenging however, due dielectric screening at physiological ionic strengths. We report here the co-engineering binding. In this strategy,...
Abstract A label free impedimetric aptasensor for simple, fast and reusable picomolar detections of Ochratoxin (OTA) in grape juices was designed. Two main factors were observed to affect the accurate toxin: i‐lateral interactions between self‐assembled aptamers ii‐ adsorption large molecules present complex matrixes like juices. Lateral minimized by constructing a Layer‐by‐Layer procedure. The interferences associated unspecific irreversible juice, reduced submitting samples ultrafiltration...
A wet method for producing vertically oriented graphene based electrodes is described.