A5101983326- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Electrochemical Analysis and Applications
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Signaling Pathways in Disease
- Catalytic Cross-Coupling Reactions
- Advanced Biosensing Techniques and Applications
- Synthesis and properties of polymers
- Mental Health Research Topics
- Monoclonal and Polyclonal Antibodies Research
- Healthcare professionals’ stress and burnout
- Biotin and Related Studies
- Health and Medical Research Impacts
- Microwave-Assisted Synthesis and Applications
- Transgenic Plants and Applications
- Molecular Junctions and Nanostructures
- Bioinformatics and Genomic Networks
- Analytical Chemistry and Sensors
- HIV/AIDS Research and Interventions
- Molecular Sensors and Ion Detection
- Computational Drug Discovery Methods
- Electrochemical sensors and biosensors
- Mentoring and Academic Development
- Virus-based gene therapy research
- DNA and Nucleic Acid Chemistry
Interface (United States)
2022-2025
Johns Hopkins University
2022-2025
Interface (United Kingdom)
2024
University of Massachusetts Amherst
2020-2022
Nanocarrier-mediated protein delivery is a promising strategy for fundamental research and therapeutic applications. However, the efficacy of current platforms into cells limited by endosomal entrapment delivered cargo with concomitantly inefficient access to cytosol other organelles, including nucleus. We report here robust, versatile polymeric–protein nanocomposite (PPNC) platform capable efficient (≥90%) proteins cytosol. synthesized library guanidinium-functionalized...
Nucleic acid-based electrochemical sensors (NBEs) can support continuous and highly selective molecular monitoring in biological fluids, both vitro vivo, via affinity-based interactions. Such interactions afford a sensing versatility that is not supported by strategies depend on target-specific reactivity. Thus, NBEs have significantly expanded the scope of molecules be monitored continuously systems. However, technology limited lability thiol-based monolayers employed for sensor...
Current strategies for the delivery of proteins into cells face general challenges endosomal entrapment and concomitant degradation protein cargo. Efficient directly to cytosol overcomes this obstacle: we report here use biotin-streptavidin tethering provide a modular approach generation nanovectors capable cytosolic biotinylated proteins. This strategy uses streptavidin organize oligo(glutamate) peptide complexes that are then electrostatically self-assembled with cationic...
Abstract Monitoring the concentration of antiretroviral drugs is critical to ensuring patient adherence HIV treatment and prevention regimens, which crucial for drug efficacy. These tools may also be useful screening samples at blood donation centers avert potential new infections. The current benchmark method support monitoring, liquid chromatography coupled mass spectrometry (LC‐MS), requires centralized facilities with costly instrumentation, has blood‐to‐result turnaround times days...
N-Heterocyclic carbenes (NHCs) are unrivaled in their ability to form persistent and tunable monolayers on noble metal surfaces, with disciplines from heterogeneous catalysis microelectronics fabrication rapidly adopting this technology. It is currently assumed that different NHC monolayer preparation protocols yield equivalent surfaces; however, a direct comparison of the leading synthetic yet validate assumption. Herein, we explore binding ligands gold (Au) surfaces prepared using five...
Real-time continuous monitoring of proteins in-vivo holds great potential for personalized medical applications. Unfortunately, a prominent knowledge gap exists in the fundamental biology regarding protein transfer and correlation between interstitial fluid blood. Additionally, technological sensing will require affinity-based platforms that cannot be robustly protected therefore challenged sensitivity, longevity, fouling over multi-day to week timelines. Here we use electrochemical aptamer...
Control over supramolecular recognition between proteins and nanoparticles (NPs) is of fundamental importance in therapeutic applications sensor development. Most NP-protein binding approaches use 'tags' such as biotin or His-tags to provide high affinity; protein surface provides a versatile alternative strategy. Generating affinity interactions challenging however, due dielectric screening at physiological ionic strengths. We report here the co-engineering binding. In this strategy,...
Abstract Problem All trainees, especially those from historically minoritized backgrounds, experience stresses that may reduce their continuation in science, technology, engineering, math, and medicine (STEMM) careers. The Johns Hopkins University School of Medicine is one ∼45 institutions with a National Institutes Health funded Postbaccalaureate Research Education Program (PREP) provides mentoring year fulltime research to prepare students excluded groups for graduate school. Having...
Self-assembled monolayers (SAMs) are employed in electrochemical biosensors to passivate and functionalize electrode surfaces. These prevent the occurrence of undesired reactions act as scaffolds for coupling bioaffinity reagents. Thiols most common adlayer used this application; however, thiol-gold bond is susceptible competitive displacement by naturally occurring solvated thiols biological fluids, well desorption under continuous voltage interrogation. To overcome these issues,...
Human cyclophilin B (CypB) is oversecreted by pancreatic cancer cells, making it a potential biomarker for early-stage disease diagnosis. Our group motivated to develop aptamer-based assays measure CypB levels in biofluids. However, human cyclophilins have been postulated collateral nuclease activity, which could impede the use of aptamers detection. To establish if can hydrolyze electrode-bound nucleic acids, we used ultrasensitive electrochemical sensors CypB's hydrolytic activity. ssDNA...
Nucleic acid-based electrochemical sensors (NBEs) have emerged as a promising approach to continuous molecular monitoring in vivo. NBEs consist of electrically conducting gold surfaces coated with self-assembled monolayers mixture electrode-passivating alkylthiols and functional alkylthiol-modified oligos. These oligos also display binding sites for the target analyte redox reporters able transfer electrons underlying electrode. Although sufficiently robust continuous, multi-hour sensing...
Nucleic acid-based electrochemical sensors (NBEs) can support continuous and highly selective molecular monitoring in biological fluids, both vitro vivo, via affinity-based interactions. Such interactions afford a sensing versatility that is not supported by strategies depend on target-specific reactivity. Thus, NBEs have significantly expanded the scope of molecules be monitored continuously systems. Although sufficiently robust for continuous, multihour small molecules, peptides, proteins...
Graphical models have gained a lot of attention recently as tool for learning and representing dependencies among variables in multivariate data. Often, domain scientists are looking specifically differences the dependency networks different conditions or populations (e.g. between regulatory species, diseased versus healthy populations). The standard method finding these is to learn each condition independently compare them. We show that this approach prone high false discovery rates (low...
Abstract Human cyclophilin B (CypB) is oversecreted by pancreatic cancer cells, making it a potential biomarker for early‐stage disease diagnosis. Our group motivated to develop aptamer‐based assays measure CypB levels in biofluids. However, human cyclophilins have been postulated collateral nuclease activity, which could impede the use of aptamers detection. To establish if can hydrolyze electrode‐bound nucleic acids, we used ultrasensitive electrochemical sensors CypB's hydrolytic...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to "Direct Cytosolic Delivery of Proteins through Coengineering and Polymeric Vehicles"Yi-Wei LeeYi-Wei LeeMore by Yi-Wei Lee, David C. LutherDavid LutherMore Lutherhttp://orcid.org/0000-0002-4697-8683, Ritabrita GoswamiRitabrita GoswamiMore Goswamihttp://orcid.org/0000-0003-3812-6322, Taewon JeonTaewon JeonMore Jeonhttp://orcid.org/0000-0003-3840-5129, Vincent ClarkVincent ClarkMore...