A5006757156- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Nanoparticle-Based Drug Delivery
- Lipid Membrane Structure and Behavior
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Advanced Nanomaterials in Catalysis
- Photonic and Optical Devices
- Quantum Dots Synthesis And Properties
- Photonic Crystals and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Dendrimers and Hyperbranched Polymers
- Carbon and Quantum Dots Applications
- Optical Coatings and Gratings
- Nanocluster Synthesis and Applications
- Polymer Surface Interaction Studies
- Molecular Sensors and Ion Detection
- Nanoplatforms for cancer theranostics
- Advanced Drug Delivery Systems
- Supramolecular Chemistry and Complexes
- Pickering emulsions and particle stabilization
- Sphingolipid Metabolism and Signaling
- Surfactants and Colloidal Systems
- Electrochemical sensors and biosensors
Indian Institute of Science Bangalore
2022-2024
University of Massachusetts Amherst
2008-2022
Chaudhary Charan Singh University
2020
Imperial College London
2013-2018
Newcastle University
2018
NIHR Imperial Biomedical Research Centre
2013-2017
The development of earth-abundant metal-oxide-based nanomaterials with an intrinsic enzyme-mimicking activity (nanozyme) is useful for both practical applications and fundamental research. laccase enzyme a multicopper oxidase that finds commercial utility in environmental remediation biotechnology, but significant limitations under harsh conditions. Herein, we present the laccase-like Cu2O nanospheres, fabricated using one-pot polyol-based microwave-assisted method. as-synthesized...
Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective faces two major challenges: efficient cellular uptake avoiding endosomal sequestration. We report here a general strategy direct of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed stabilized through supramolecular interactions between nanoparticle, cargo, fatty acid capsule interior. The ~130 nm in diameter feature low...
A versatile and sensitive colorimetric assay that allows the rapid detection of small-molecule targets using naked eye is demonstrated. The working principle integrates aptamer–target recognition aptamer-controlled growth gold nanoparticles (Au NPs). Aptamer–target interactions modulate amount aptamer strands adsorbed on surface aptamer-functionalized Au NPs via desorption when target molecules bind with aptamer. Depending resulting coverage, grow into morphologically varied nanostructures,...
Supramolecular complexes of a family positively charged conjugated polymers (CPs) and green fluorescent protein (GFP) create fluorescence resonance energy transfer (FRET)-based ratiometric biosensor array. Selective multivalent interactions the CPs with mammalian cell surfaces caused differential change in FRET signals, providing fingerprint signature for each type. The resulting signatures allowed identification 16 different types discrimination between healthy, cancerous, metastatic cells,...
Rapid and sensitive methods of discriminating between healthy tissue metastases are critical for predicting disease course designing therapeutic strategies. We report here the use an array gold nanoparticle-green fluorescent protein elements to rapidly detect metastatic cancer cells (in minutes), as well discriminate organ-specific their corresponding normal tissues through overall intracellular proteome signatures. Metastases established in a new preclinical non-small-cell lung metastasis...
We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform host–guest interactions between cucurbit[7]uril and cationic headgroup gold nanoparticle non-covalently modify binding three fluorescent proteins multi-channel sensor in situ. approach doubles number output channels six, providing single-well identification with 100% accuracy. Significantly, this classification could be extended beyond training set, determining...
The binding of bovine serum albumin (BSA) and β-lactoglobulin (BLG) to TTMA (a cationic gold nanoparticle coupled 3,6,9,12-tetraoxatricosan-1-aminium, 23-mercapto-N,N,N-trimethyl) was studied by high-resolution turbidimetry (to observe a critical pH for binding), dynamic light scattering monitor particle growth), isothermal titration calorimetry measure energetics), all as function ionic strength. Distinctively higher affinities observed BLG versus BSA, despite the lower pI latter, were...
Small but mighty: Current methods for generating oil-in-water emulsions provide nanoparticle-stabilized capsules (NPSCs) that are too large efficient delivery. Electrostatic and hydrogen-bonding interactions were combined to create stable NPSCs with diameters of around 100 nm. Their potential as delivery vehicles was demonstrated through dye studies HeLa cells. Detailed facts importance specialist readers published "Supporting Information". Such documents peer-reviewed, not copy-edited or...
Gold nanoparticle-green fluorescent protein (NP-GFP) based arrays have been created for rapid identification of mammalian cells on the basis cell surface properties. Highly reproducible characteristic patterns were obtained from different types enabling and cancer states. Using these we could differentiate between isogenic normal, metastatic using only ∼5000 cells.
Identification of infectious bacteria responsible for biofilm-associated infections is challenging due to the complex and heterogeneous biofilm matrix. To address this issue minimize impact heterogeneity on identification, we developed a gold nanoparticle (AuNP)-based multichannel sensor detect identify biofilms based their physicochemical properties. Our results showed that can discriminate six bacterial including two composed uropathogenic bacteria. The capability was further demonstrated...
Fundamentally different assembly motifs are observed when proteins of sizes complexed with monolayer-protected nanoparticles.
The development of superior functional enzyme mimics (nanozymes) is essential for practical applications, including point-of-care diagnostics, biotechnological biofuels, and environmental remediation. Nanozymes with the ability to control their catalytic activity in response external fuels offer functionally valuable platforms mimicking nonequilibrium systems nature. Herein, we fabricated a supramolecular coordination bonding-based dynamic vesicle that exhibits multienzymatic activity....
In prokaryotes, the energy-dependent protein degradation is controlled primarily by two ATP-dependent proteases, Lon and Clp. This study investigates roles of protease in metabolism 2,4-dinitrophenol (2,4-DNP), a toxic industrial compound, Escherichia coli (E. coli). During study, an observation was made that absence resulted enhanced conversion yellow coloured 2,4-DNP to reddish-brown product. aims characterise compound observed media with wild type (WT) Δlon strains, understand mechanisms...
A sensor array composed of eight functionalized gold nanoparticles and a fluorescent polymer was utilized to identify 11 glycosaminoglycans (GAGs) by their chemical signatures. Particle ligand structure key in differentiating analytes, with aromatic specific sugar functionalities recognizing GAG structural parameters including sulfonation, acetylation, epimeric structure, size, charge.
Cell surface glycosylation serves a fundamental role in dictating cell and tissue behavior. glycomes differ significantly, presenting viable biomarkers for identifying types their states. Glycoprofiling is challenging task, however, due to the complexity of constituent glycans. We report here rapid effective sensor surface-based differentiation that uses three-channel produced by noncovalent conjugation functionalized gold nanoparticle (AuNP) fluorescent proteins. Wild-type glycomutant...