A5087040030- Microfluidic and Capillary Electrophoresis Applications
- Pain Management and Opioid Use
- Pharmacogenetics and Drug Metabolism
- Mangiferin and Mango Extracts
- Molecular Junctions and Nanostructures
- Bacteriophages and microbial interactions
- Marine Sponges and Natural Products
- Pain Mechanisms and Treatments
- Advanced biosensing and bioanalysis techniques
- Quantum Dots Synthesis And Properties
- BRCA gene mutations in cancer
- Aquaculture disease management and microbiota
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Pain Management and Placebo Effect
- Ginger and Zingiberaceae research
- Seaweed-derived Bioactive Compounds
- Plant Genetic and Mutation Studies
- Opioid Use Disorder Treatment
- Innovative Microfluidic and Catalytic Techniques Innovation
- Surface and Thin Film Phenomena
- Phytochemistry and biological activity of medicinal plants
- Cholinesterase and Neurodegenerative Diseases
- Health and Lifestyle Studies
- Natural Compound Pharmacology Studies
Washburn University
2025
University of Havana
2015-2025
Instituto de Investigaciones en Ciencia y Tecnología de Materiales
2016
Hospital Universitario de Valme
2005-2012
Hospital Materno-Infantil
1998-2007
Cuban Neuroscience Center
1994-2006
Southern Polytechnic State University
2002
Research Institute for Fragrance Materials
1984
United States Department of Commerce
1978
University of Chile
1971
Single-nucleotide variants (SNVs) give rise to important inter-individual and inter-ethnic variabilities in the metabolism disposition of several therapeutic agents may cause differences treatment response clinically drugs like antiarrhythmics, antidepressants, antihistamines, antipsychotics, among others. Information about prevalence Dominican Republic population is still limited. The aim this study was describe frequency distribution 32 SNVs from 14 genes with pharmacogenetic interest...
Context: Pharmacological properties of mangiferin have been reported, but few studies investigated toxicity. Aims: To study the acute and 28-day toxicity effects in rodents. Methods: Single doses were administered by oral or i.p. route applied dermally to Sprague-Dawley rats Balb/C mice. Clinical symptoms animals observed during 14 days after treatment. Animals also received single daily for 28 consecutive days. Blood biochemistry, hematology pathology findings reported. Results: In study,...
Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) colon tumor cell lines (RKO, SW480, and CT26) syngeneic allograft murine colorectal model. MTT assays revealed dose-dependent decrease viability RKO, CT26, SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, 60 μg/mL. Furthermore,...
The aqueous stem bark extract of Mangifera indica L. (MSBE) has been reported to have antioxidant, anti‐inflammatory and analgesic properties. In previous studies, we showed that MSBE mangiferin, its main component, lower the activity some cytochrome P‐450 (P450) enzymes in rat hepatocytes human liver microsomes. present study, effects mangiferin on several P450 UDP‐glucuronosyltransferases (UGTs) human‐cultured examined. After underwent a 48‐h treatment with sub‐cytotoxic concentrations...
The chemical composition and biological properties of Ulva fasciata aqueous-ethanolic extract were examined. Five components identified in one fraction prepared from the by gas chromatography-mass spectrometry, palmitic acid its ethyl ester accounted for 76% total components. Furthermore, we assessed extract's antioxidant using DPPH, ABTS, lipid peroxidation assays found that had a moderate scavenging effect. In an experiment involving preexposition coexposition (1-500 µg/mL) benzo[a]pyrene...
Abstract Objective Mango (Mangifera indica L.) stem bark extract (MSBE) is a natural product with biological properties and mangiferin the major component. This paper reported evaluation of protective effects MSBE against toxicity induced in HepG2 cells by tert-butyl hydroperoxide or amiodarone. Method Nuclear morphology, cell viability, intracellular calcium concentration reactive oxygen species (ROS) production were measured using high-content screening multiparametric assay. Key findings...
The chronic toxicologic and carcinogenic potential of two oxidative twelve non-oxidative hair dyes has been evaluated. were skin painted up to 3 times weekly on groups 60 male female Eppley Swiss mice. Treatments carried out for 20 months followed by terminal sacrifice. Nine after treatments initiated an intermediate sacrifice ten mice per sex group was out. Body weights survival differed little between appropriate treatment control groups. Differences treated in absolute relative liver...
Context: Marine ecosystems are sources of bioactive compounds. Thirty-eight microorganism strains from the Cuban platform were screened, which allowed us to identify an extract Bacillus amyloliquefaciens, strain CBM-116, as a source for obtaining bioproducts with biomedical applications. Aims: To physiologically characterize culture amyloliquefaciens (CBM-116 strain) and evaluate antioxidant toxic potentialities in vitro M116 obtained CBM-116. Methods: The growth metabolite production...
The protective effect of the supplementation with an aqueous‐ethanolic extract obtained from Ulva lactuca (Delile) green seaweed on benzo[ a ] pyrene‐induced damage in mice was evaluated. Animals were treated oral doses U. (100 and 400 mg/kg) for 9 weeks. They exposed to 50 mg/kg benzo(a)pyrene starting second week up fifth week. Groups only (second weeks), sunflower oil (vehicle, or mg/kg, weeks) also included study. treatment ameliorated oxidative damage, decreased IL‐1β TNF‐α levels,...
Introduction The Cuban population is genetically diverse, and information on the prevalence of genetic variants still limited. As complex admixture processes have occurred, we hypothesized that frequency pharmacogenetic drug responses may vary within country. aims study were to describe distribution 43 single-nucleotide (SNVs) from 25 genes interest Cuba in relation other populations, while taking into consideration some descriptive variables such as place birth skin color. Materials Methods...
Abstract Objectives Reported antioxidant, anti-inflammatory and neuroprotective properties for one aqueous-ethanolic extract from Thalassia testudinum which grows in the Caribbean Sea compelled us to explore about cytotoxic effects. Methods Cell viability was assayed on tumour (HepG2, PC12, Caco-2 4T1) non-tumour (VERO, 3T3, CHO, MCDK BHK2) cell lines. The effects upon primary cultures of rat human hepatocytes lymphocytes were assayed. Key findings exhibited cytotoxicity against cancer cells...
DNA molecules of Vibrio cholerae and Aeromonas species were prepared by incubating immobilized cells for 4 2 h, respectively, with a nonenzymatic solution that contains chemical reagents only (NDSUPlus). This method gave results as reproducible the enzymatic one uses proteinase K, rendered suitable fingerprinting mini-CHEF electrophoresis. As rapid separations at high electric field are achieved in chamber low heat evolution, restriction fragments separated 5 h 10 V/cm single resolution...
Abstract Pulsed field gel electrophoresis runs were performed in small Contour Clamped Homogeneous Electric Field (miniCHEF) and Transverse Alternating (miniTAFE) chambers. Minichambers use minigels they could be suitable for analytical preparative purposes. In minichambers higher electric fields equal heat evolution achieved with the same voltage applied to conventional Nine ten Saccharomyces cerevisiae chromosomes resolved applying 13–14 V/cm 50 s of pulse time during 3.2 - 5 h. It was...
The aim of the present work was to evaluate effects Thalassia testudinum hydroethanolic extract, its polyphenolic fraction and thalassiolin B on activity phase I metabolizing enzymes as well their antimutagenic effects. Spectrofluorometric techniques were used effect tested products rat human CYP1A CYP2B activity. evaluated in benzo[a]pyrene (BP)-induced mutagenicity assay by an Ames test. Finally, (100 mg/kg) assessed BP-induced mutagenesis mice. significantly (p < 0.05) inhibit CYP1A1...
The effects of an extract from the marine plant Thalassia testudinum on rat liver cytochrome P450s were investigated.Male Wistar rats administered for 10 days with 20, 200 or 400 mg/kg oral doses extract.The activities CYP1A1/A2, CYP2B1/B2, CYP2E1 and CYP3A evaluated.CYP1A1 activity protein content increased after but mRNA levels remain unchanged.The other CYP isoforms did not change.The S9 fraction derived livers treated was used to evaluate this product mutagenic activation...
The expansion of the uppermost gold layer an unreconstructed Au(100) surface is expected upon sulphur adsorption.