A5086373039- Pharmacogenetics and Drug Metabolism
- Liver physiology and pathology
- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Pancreatic function and diabetes
- Metabolomics and Mass Spectrometry Studies
- 3D Printing in Biomedical Research
- Computational Drug Discovery Methods
- Eicosanoids and Hypertension Pharmacology
- Mangiferin and Mango Extracts
- Pluripotent Stem Cells Research
- Ginger and Zingiberaceae research
- Nitric Oxide and Endothelin Effects
- Liver Disease and Transplantation
- Carcinogens and Genotoxicity Assessment
- Innovative Microfluidic and Catalytic Techniques Innovation
- Tissue Engineering and Regenerative Medicine
- Animal testing and alternatives
- Antibiotics Pharmacokinetics and Efficacy
- Chemical Reactions and Isotopes
- Cell death mechanisms and regulation
- Endoplasmic Reticulum Stress and Disease
- Cell Image Analysis Techniques
Instituto de Investigación Sanitaria La Fe
2016-2025
Universitat de València
2016-2025
Centro de Investigación Biomédica en Red
2007-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2012-2024
Instituto de Salud Carlos III
2016-2024
Universidad de León
2015
CIC bioGUNE
2015
Weatherford College
2008
Hospital Universitari i Politècnic La Fe
1994-2006
Leitat Technological Center
2006
1. Cultured hepatic cells have reduced cytochrome P450 (CYP) activities in comparison with human liver, but the mechanism(s) that underlies this circumstance is not clear. We investigated causes of low CYP activity by analysing activity, protein, mRNA and heterologous nuclear RNA contents most important CYPs involved drug metabolism (1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, 3A4, 3A5) cultured hepatocytes, HepG2 Mz-Hep-1 hepatoma cell lines. 2. After 24 h culture, hepatocytes retained their...
Bile acids (BAs) are a group of chemically related steroids recognized as regulatory molecules whose profiles can change in different physio-pathological situations. We have developed sensitive, fast, and reproducible ultraperformance liquid chromatography/multiple reaction monitoring/mass spectrometry method to determine the tissue sera BA species (human, rat, mouse) by quantifying 31 major minor single 21-min run. The has been validated according FDA guidelines, it generally provides good...
In this study we describe a battery of fluorescence assays for rapid measurement in intact cells the activity nine cytochromes P450 (P450s) involved drug metabolism. The are based on direct incubation monolayers expressing individual enzymes with fluorogenic substrate followed by fluorimetric quantification product formed and released into medium. For each activity, different probes were examined, one showing best properties (highest metabolic rates, lowest background fluorescence) was...
Steatosis, or excessive accumulation of lipids in the liver, is a generally accepted previous step to development more severe conditions like nonalcoholic steatohepatitis, fibrosis, and cirrhosis. We aimed characterize metabolic profile that defines simple steatosis human tissue identify potential disturbances hepatic metabolism could favor switch progressive liver damage. A total 46 samples, 23 from steatotic nonsteatotic livers, were analyzed following holistic LC-MS-based metabonomic...
Hepatotoxicity is a major reason for drug nonapprovals and withdrawals. The multiparametric analysis of xenobiotic toxicity at the single cells level using flow cytometry cellular imaging–based approaches, such as high-content screening (HCS) technology, could play key role in detection classification compounds based on patterns injury. This study aimed to develop validate practical, reproducible, vitro cell-based protocol assess those drugs that are potentially hepatotoxic humans suggest...
Hepatocytes entrapped in collagen gel and cultured serum-free conditions survived longer than cells on plastic (5 days vs. 3 weeks), showed fewer signs of early cell senescence (no increase c-fos oncoprotein expression), maintained the expression differentiated hepatic metabolic functions over a period time. Cells gels retained their ability to respond hormones. The insulin-stimulated glycogen synthesis rate remained fairly constant during 18 culture (between 5.4 ± 0.37 9 2.7 nmol...
Liver grafts discarded for transplantation because of macrosteatosis can constitute a valuable source human hepatocytes in vitro metabolic and pharmacotoxicological studies or therapeutic applications. A condition using hepatocyte suspensions these purposes is the preservation their competence and, particularly, drug-metabolizing enzymes. reduction microsomal cytochrome P450 (P450) activities was observed fatty livers (>40% steatosis) with respect to normal tissue. Similarly, decreased...