A5006789617- Pharmacogenetics and Drug Metabolism
- Drug-Induced Hepatotoxicity and Protection
- Pluripotent Stem Cells Research
- Drug Transport and Resistance Mechanisms
- Liver physiology and pathology
- Computational Drug Discovery Methods
- Pancreatic function and diabetes
- Animal testing and alternatives
- Neurogenesis and neuroplasticity mechanisms
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
- Cannabis and Cannabinoid Research
- Alcohol Consumption and Health Effects
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Liver Disease Diagnosis and Treatment
- Heavy Metal Exposure and Toxicity
- Inflammatory mediators and NSAID effects
- Axon Guidance and Neuronal Signaling
- Hereditary Neurological Disorders
- Eicosanoids and Hypertension Pharmacology
- Folate and B Vitamins Research
- Neurobiology and Insect Physiology Research
Universitat de València
2010-2024
INCLIVA Health Research Institute
2020
Hospital Universitari i Politècnic La Fe
1989-2001
Instituto de Investigación Sanitaria La Fe
1990-2001
Biosensores (Spain)
2000
Leitat Technological Center
1991
Abstract During the process of reprogramming to induced pluripotent stem (iPS) cells, somatic cells switch from oxidative glycolytic metabolism, a transition associated with profound mitochondrial reorganization. Neither importance remodelling for cell reprogramming, nor molecular mechanisms controlling this are well understood. Here, we show that an early wave fragmentation occurs upon expression factors. Reprogramming-induced fission is minor decrease in mass but not mitophagy. The...
Diclofenac, a 2-arylacetic acid, nonsteroidal anti-inflammatory drug, has been reported to cause adverse hepatic effects in certain individuals. To discriminate among possible mechanisms of hepatotoxicity, we examined the diclofenac on human and rat hepatocytes cell lines (HepG2, FaO), investigated major biochemical events course cytotoxicity (calcium homeostasis, lipid peroxidation, mitochondrial dysfunction), whether could be related drug metabolism by cytochrome P-450. Acute...
Hepatocytes entrapped in collagen gel and cultured serum-free conditions survived longer than cells on plastic (5 days vs. 3 weeks), showed fewer signs of early cell senescence (no increase c-fos oncoprotein expression), maintained the expression differentiated hepatic metabolic functions over a period time. Cells gels retained their ability to respond hormones. The insulin-stimulated glycogen synthesis rate remained fairly constant during 18 culture (between 5.4 ± 0.37 9 2.7 nmol...
The phenolic phytoalexin resveratrol is well known for its health-promoting and anticancer properties. Its potential benefits are, however, limited due to low bioavailability. Pterostilbene, a natural dimethoxylated analog of resveratrol, presents higher activity than resveratrol. mechanisms by which this polyphenol acts against cancer cells unclear. Here, we show that pterostilbene effectively inhibits cell growth stimulates apoptosis autophagosome accumulation in various origins. However,...
The MEIC (multicentre evaluation of in vitro cytotoxicity) programme is a five-year to validate tests for general toxicity, and organised by the Scandinavian Society Cell Toxicology. Interested laboratories are invited, on an international basis, test 50 published reference chemicals their respective assays. Submitted results will then be evaluated yearly Committee relevance various types human including same prediction animal toxicity. To establish validation methods, preliminary cycle...
The BC2 cell line derived from the human hepatocarcinoma, HGB, undergoes a spontaneous sharp differentiation process in culture as it becomes confluent, remains stably differentiated for several weeks, and may return to proliferation thereafter under appropriate density conditions. relevance of an hepatic model has been evaluated. Cells synthesize large number plasma proteins, rates glycogen urea synthesis increase with time confluency become sensitive insulin, reflecting differentiation....
Metabolism of the new nonsteroidal antiinflammatory drug aceclofenac ([2-(2',6'-dichlorophenylamino)phenyl]acetoxyacetic acid) was investigated both in vitro hepatic human models and vivo. Aceclofenac is metabolized hepatocytes microsomes to form [2-(2',6'-dichloro-4'-hydroxy- phenylamino)phenyl]acetoxyacetic acid as major metabolite, which then further conjugated. Minor metabolites were [2-(2',6'-dichlorophenylamino)-5-hydroxyphenyl]acetoxyacetic [2-(2',6'-dichlorophenylamino)phenyl]acetic...
The organization and dynamics of microtubules (MTs) the actin cytoskeleton are critical for correct development functions neurons, including intracellular traffic signaling. In vitro ethanol exposure impairs endocytosis, exocytosis, nucleocytoplasmic in astrocytes alters endocytosis cultured neurons. astrocytes, these effects relate to changes and/or function MTs cytoskeleton. To evaluate this possibility hippocampal we analyzed if chronic affects levels, assembly, cellular both elements...
We have recently shown that mitochondrial fission is induced early in reprogramming a Drp1-dependent manner; however, the identity of factors controlling Drp1 recruitment to mitochondria was unexplored. To investigate this, we used panel RNAi targeting involved regulation dynamics and observed MiD51, Gdap1 and, lesser extent, Mff were found play key roles this process. Cells derived from Gdap1-null mice further explore role factor cell reprogramming. Microarray data revealed prominent...
Cell reprogramming is thought to be associated with a full metabolic switch from an oxidative- glycolytic-based metabolism. However, neither the dynamics nor factors controlling this are fully understood. By using cellular, biochemical, protein array, metabolomic, and respirometry analyses, we found that c-MYC establishes robust bivalent energetics program early in cell reprogramming. Cells prone undergo exhibit high mitochondrial membrane potential display hybrid We conclude MYC proteins...
Charcot-Marie-Tooth disease is a chronic hereditary motor and sensory polyneuropathy targeting Schwann cells and/or neurons. Its multifactorial polygenic origin portrays complex clinical phenotype of the with wide range genetic inheritance patterns. The disease-associated gene GDAP1 encodes for mitochondrial outer membrane protein. Mouse insect models mutations in Gdap1 have reproduced several traits human disease. However, precise function cell types affected by remains unknown. Here, we...
Endocytosis is required for many cellular pivotal processes, including membrane recycling, nutrient uptake, and signal transduction. This complex process particularly relevant in polarized cells, such as neurons. Previous studies have demonstrated that alcohol alters intracellular traffic, endocytosis, several cell types. However, information on the effect of chronic exposure this neurons scarce. As an approach, we investigated internalization two widely used endocytic markers, albumin...