Development of a bioartificial renal tubule with confluent monolayer epithelial cells supported on permeable synthetic surface may be the first step to further optimization substitution therapy currently used hemodialysis or hemofiltration. Madin-Darby canine kidney cells, permanent cell line, were seeded into lumen single hollow fibers. Functional confluence was demonstrated by recovery intraluminally perfused 14C-inulin that averaged >98.9% in lined units vs < 7.4% control noncell fibers...

ABSTRACT. The bioartificial kidney (BAK) consists of a conventional hemofiltration cartridge in series with renal tubule assist device (RAD) containing 109 porcine proximal cells. BAK replaces filtration, transport, and metabolic endocrinologic activities kidney. Previous work an acutely uremic dog model demonstrated that ameliorated endotoxin (lipopolysaccharide [LPS])-induced hypotension altered plasma cytokine levels. To further assess the role sepsis acute failure, dogs were...

Renal cell therapy has shown clinical efficacy in the treatment of acute renal failure (ARF) and promise for end-stage disease (ESRD) by supplementing conventional small solute clearance (hemodialysis or hemofiltration) with endocrine metabolic function provided cells maintained an extracorporeal circuit. A major obstacle widespread adoption this therapeutic approach is lack a cryopreservable system to enable distribution, storage, use at point care facilities. This report details design,...

Objective Gram-negative septic shock has a clinical mortality rate approaching 50%. The cause of death is secondary to systemic inflammatory response syndrome with resulting cardiovascular collapse, ischemic damage vital organs, and multiple-organ systems failure. Renal tubule cell injury occurs early in but not clinically appreciated. Since renal cells appear play critical role the immunoregulation stress states, therapy during may alter detrimental consequences infection. development...

The mortality from sepsis complicated by renal failure remains extremely high despite the application of modern replacement therapy. This study investigated whether treatment with a bioartificial kidney consisting hemofilter in continuous venovenous hemofiltration circuit (CVVH) cartridge containing proximal tubule cells, also called Renal Tubule Assist Device (RAD), would alter course an animal model. RAD has been previously characterized vitro and ex vivo provides transport, metabolic...

Objective Septic shock has a clinical mortality rate approaching fifty percent. The major manifestations of sepsis are due to the dysregulation host's response infection rather than direct consequences invading pathogen. Central this initial immunologic is activation leukocytes and microvascular endothelium resulting in cardiovascular instability, lung injury renal dysfunction. Due primary role leukocyte syndrome, synthetic biomimetic membrane, called selective cytopheretic device (SCD), was...

Cell therapy for the treatment of renal failure in acute setting has proved successful, with therapeutic impact, yet development a sustainable, portable bioartificial kidney chronic to be realized. Challenges maintaining an anticoagulated blood circuit, typical platform solute clearance and support biological components, have posed major hurdle advancement this technology. This group developed Bioartificial Renal Epithelial System (BRECS) capable differentiated cell function while sustained...

Renal cell therapy employing cells derived from adult renal epithelial (REC) progenitors promises to reduce the morbidity of patients with insufficiency due acute failure and end stage disease. To this end, tissue engineered devices addressing neglected biologic component replacement are being developed. Because human donor is limited, novel enhanced progenitor propagation (EP) techniques have been developed applied kidney transplant discards six donors. Changes include more efficient...

Renal cell therapy using the hollow fiber based renal assist device (RAD) improved survival time in an animal model of septic shock (SS) through amelioration cardiac and vascular dysfunction. Safety ability RAD to improve clinical outcomes was demonstrated a Phase II trial, which patients had high prevalence sepsis. Even with these promising results, delivery is hampered by manufacturing hurdles, including sourcing, large-scale manufacture, storage delivery. To address limitations,...

Patients with end-stage renal disease (ESRD) on chronic hemodialysis (HD) suffer accelerated morbidity and mortality rates caused by cardiovascular infections. Chronic inflammation plays a critical role in these poor outcomes. The activated monocyte (MO) has become prime therapeutic target to modulate this inflammatory process. A selective cytopheretic device (SCD) was evaluated assess its effects the circulating MO pool. pilot trial undertaken 15 ESRD patients HD C-reactive protein (CRP)...

The bioartificial renal epithelial cell system (BRECS) is a cell-based device to treat acute kidney injury through therapy from an extracorporeal circuit. To enable widespread implementation of therapy, the BRECS was designed be cryopreserved as complete device, cryostored, cryoshipped end-use site, thawed and employed in therapeutic hemofiltration This strategy overcomes storage distribution issues that have been previous barriers therapy. Previous housings produced by computer numerical...

Background Inflammation has been associated with progression and complications of chronic heart failure (HF) but no effective therapy yet identified to treat this dysregulated immunologic state. The selective cytopheretic device (SCD) provides extracorporeal autologous cell processing lessen the burden inflammatory activity circulating leukocytes innate system. Aim objective study was evaluate effects SCD as an immunomodulatory on immune state HF. Methods results treatment in a canine model...

Obesity is associated with tissue inflammation which a crucial etiology of insulin resistance. This centers around circulating monocytes form proinflammatory adipose macrophages (ATM). Specific approaches targeting monocytes/ATM may improve resistance without the adverse side effects generalized immunosuppression. In this regard, biomimetic membrane leukocyte processing device, called selective cytopheretic device (SCD), was evaluated in an Ossabaw miniature swine model metabolic syndrome....

The first step in the tissue engineering of an implantable bioartificial kidney is development implant that produces ultrafiltrate to replace glomerular function. A fabricated device containing synthetic hollow hemofiltration fibers was placed around femoral vascular pedicle rats, which initiated new formation with a mature and durable neocapillary bed. transudate fluid produced by this newly formed capillary bed accumulated through into subcutaneous port allow evaluation fluid. In its...

Leukocyte (LE) activation during cardiopulmonary bypass (CPB) promotes a systemic inflammatory response that contributes to organ injury and postoperative dysfunction. A leukocyte modulatory device (L-MOD) for use (and after) CPB limit leukocyte-mediated was tested in preclinical model. Twenty-two pigs underwent 180 minutes of 5 hours observation. Pigs received no intervention (group 1, n = 9), 3 therapy by incorporation L-MOD into the circuit 2, 6), or 8 using femoral venovenous after 3,...