A5032436944- Advanced Sensor and Energy Harvesting Materials
- Bacterial biofilms and quorum sensing
- Central Venous Catheters and Hemodialysis
- Antimicrobial agents and applications
- Sepsis Diagnosis and Treatment
- Medical Imaging and Analysis
- Pancreatic function and diabetes
- Gut microbiota and health
- Wastewater Treatment and Nitrogen Removal
- Ophthalmology and Visual Impairment Studies
- Conducting polymers and applications
- Analytical Chemistry and Sensors
- Nitric Oxide and Endothelin Effects
- Cardiac Valve Diseases and Treatments
- Microbial bioremediation and biosurfactants
- Electrospun Nanofibers in Biomedical Applications
- Nanoplatforms for cancer theranostics
- Ultrasound in Clinical Applications
- Corneal surgery and disorders
- Spine and Intervertebral Disc Pathology
- Chemical Synthesis and Analysis
- Connexins and lens biology
- Hemodynamic Monitoring and Therapy
- Intraocular Surgery and Lenses
- Antimicrobial Peptides and Activities
Union Hospital
2024-2025
Pingjin Hospital
2024-2025
Fujian Medical University
2024-2025
University of Michigan
2014-2024
Xiamen University
2018-2024
University of Technology
2024
Fujian University of Technology
2024
The First Affiliated Hospital, Sun Yat-sen University
2016-2023
Sun Yat-sen University
2016-2023
Michigan United
2023
Abstract Metformin, the most prescribed antidiabetic medicine, has shown other benefits such as anti-ageing and anticancer effects 1–4 . For clinical doses of metformin, AMP-activated protein kinase (AMPK) a major role in its mechanism action 4,5 ; however, direct molecular target metformin remains unknown. Here we show that clinically relevant concentrations inhibit lysosomal proton pump v-ATPase, which is central node for AMPK activation following glucose starvation 6 We synthesize...
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A glycine- and leucine-based tripeptide improves symptoms in a mouse model of nonalcoholic fatty liver disease, which lacks approved treatments.
p38 mitogen-activated protein kinases are important inflammatory factors. p38α alteration has been implicated in both human and mouse disease models. Therefore, we aimed to characterize the cell type-specific role of non-alcoholic steatohepatitis (NASH).Human liver tissues were obtained from 27 patients with fatty (NAFLD) 20 control individuals. NASH was established compared between hepatocyte-specific knockout (p38αΔHep), macrophage-specific (p38αΔMΦ) wild-type (p38αfl/fl) mice fed high-fat...
ABSTRACT The occurrence of high concentrations extracellular DNA (eDNA) in the matrices biofilms plays an important role biofilm formation and development possibly horizontal gene transfer through natural transformation. Studies have been conducted to characterize nature eDNA its potential function development, but it is difficult extract from without any contamination genomic released by cell lysis during extraction process. In this report, we compared several different methods order obtain...
Heavy metal pollution is a principle source of environmental contamination. Epidemiological and animal data suggest that early life lead (Pb) exposure results in critical effects on epigenetic gene regulation child adult weight trajectories. Using mouse model human-relevant exposure, we investigated the perinatal Pb gut microbiota mice, link between bodyweight changes. Following during gestation lactation via maternal drinking water, Avy strain wild-type non-agouti (a/a) offspring was...
Fructose-1,6-bisphosphate (FBP) aldolase links sensing of declining glucose availability to AMPK activation via the lysosomal pathway. However, how transmits lack occupancy by FBP remains unclear. Here, we show that FBP-unoccupied interacts with and inhibits endoplasmic reticulum (ER)-localized transient receptor potential channel subfamily V, inhibiting calcium release in low glucose. The decrease at contact sites between ER lysosome renders inhibited TRPV accessible bind v-ATPase then...
Graphene and nanomaterials based flexible pressure sensors R&D activities are becoming hot topics due to the huge marketing demand on wearable devices electronic skin (E-Skin) monitor human body's actions for dedicated healthcare. Herein, we report a facile efficient fabrication strategy construct new type of highly sensitive E-Skin graphite nanoplates (GNP) polyurethane (PU) nanocomposite films. The developed GNP/PU with good electrical conductivity their unique binary microstructures...
The prolonged and localized delivery of nitric oxide (NO), a potent antithrombotic antimicrobial agent, has many potential biomedical applications. In this work, the origin long-term storage stability sustained NO release mechanism S-nitroso-N-acetyl-d-penicillamine (SNAP)-doped CarboSil 20 80A polymer, thermoplastic silicone-polycarbonate-urethane, is explored. Long-term (22 days) achieved by utilizing cross-linked silicone rubber as topcoats, which can greatly reduce amount SNAP, NAP, NAP...
The activity of 5'-adenosine monophosphate-activated protein kinase (AMPK) is inversely correlated with the cellular availability glucose. When glucose levels are low, glycolytic enzyme aldolase not bound to fructose-1,6-bisphosphate (FBP) and, instead, signals activate lysosomal AMPK. Here, we show that blocking FBP binding small molecule aldometanib selectively activates pool AMPK and has beneficial metabolic effects in rodents. We identify a screen for inhibitors it prevents from...
Lithocholic acid (LCA) is accumulated in mammals during calorie restriction and it can activate AMP-activated protein kinase (AMPK) to slow down ageing1. However, the molecular details of how LCA activates AMPK induces these biological effects are unclear. Here we show that enhances activity sirtuins deacetylate subsequently inhibit vacuolar H+-ATPase (v-ATPase), which leads activation through lysosomal glucose-sensing pathway. Proteomics analyses proteins co-immunoprecipitated with sirtuin...
ABSTRACT Comamonas sp. strain CNB-1 grows on 4-chloronitrobenzene (4-CNB) and nitrobenzene as sole carbon nitrogen sources. In this study, two genetic segments, cnbB-orf2-cnbA cnbR-orf1-cnbCaCbDEFGHI , located a newly isolated plasmid, pCNB1 (ca. 89 kb), involved in 4-CNB/nitrobenzene degradation, were characterized. Seven genes ( cnbA cnbB cnbCa cnbCb cnbD cnbG cnbH ) cloned functionally expressed recombinant Escherichia coli they identified encoding 4-CNB nitroreductase (CnbA),...
Urinary Foley catheters are utilized for management of hospitalized patients and associated with high rates urinary tract infections (UTIs). Nitric oxide (NO) potently inhibits microbial biofilm formation, which is the primary cause catheter UTIs (CAUTIs). Herein, commercial silicone impregnated via a solvent swelling method S-nitroso-N-acetyl-D-penicillamine (SNAP), synthetic NO donor that exhibits long-term release stability when incorporated into low water-uptake polymers. The proposed...
Abstract Pathogenic microorganisms often have the ability to attach a surface, building complex matrix where they colonize form biofilm. This cellular superstructure can display increased resistance antibiotics and cause serious, persistent health problems in humans. Here we describe high-throughput vitro screen identify inhibitors of Acinetobacter baumannii biofilms using library natural product extracts derived from marine microbes. Analysis Streptomyces gandocaensis results discovery...
Silver nanoparticles (AgNPs), the most prevalent commercial nanomaterial products, are widely released into environment.
The human opportunistic pathogen, Acinetobacter baumannii, has the propensity to form biofilms and frequently cause medical device-related infections in hospitals. However, physio-chemical properties of surfaces, addition bacterial surface properties, will affect colonization biofilm development. objective this study was compare ability A. baumannii on six different materials common hospital environment: glass, porcelain, stainless steel, rubber, polycarbonate plastic polypropylene plastic....
Nitric oxide (NO) has many important physiological functions, including its ability to inhibit platelet activation and serve as potent antimicrobial agent. The multiple roles of NO in vivo have led great interest the development biomaterials that can deliver for specific biomedical applications. Herein, we report a simple solvent impregnation technique incorporate nontoxic donor, S-nitroso-N-acetylpenicillamine (SNAP), into more biocompatible grade polymer, CarboSil 20 80A. resulting...
The majority of men with defects in spermatogenesis remain undiagnosed. Acephalic spermatozoa is one the diseases causing primary infertility. However, causes underlying over half affected cases unclear. Here, we report by whole‐exome sequencing identification homozygous and compound heterozygous truncating mutations PMFBP1 two unrelated individuals acephalic spermatozoa. was highly specifically expressed human mouse testis. Furthermore, immunofluorescence staining sperm from a normal...