A5100368853- Liver Disease Diagnosis and Treatment
- Gut microbiota and health
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Endoplasmic Reticulum Stress and Disease
- Metabolomics and Mass Spectrometry Studies
- Liver physiology and pathology
- Lipid metabolism and disorders
- Liver Disease and Transplantation
- Liver Diseases and Immunity
- Pancreatitis Pathology and Treatment
- Cancer, Lipids, and Metabolism
- Hepatitis C virus research
- Ferroptosis and cancer prognosis
- Drug Transport and Resistance Mechanisms
- Autophagy in Disease and Therapy
- Helicobacter pylori-related gastroenterology studies
- Hepatitis B Virus Studies
- Colorectal Cancer Treatments and Studies
- MRI in cancer diagnosis
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Animal Virus Infections Studies
- Cancer, Hypoxia, and Metabolism
Chinese University of Hong Kong
2016-2025
Chinese University of Hong Kong, Shenzhen
2014-2025
Air Force Medical University
2024
Xinzhou Teachers University
2023
Chongqing Medical University
2016-2023
Digestive Health Research Institute
2017-2023
Yeungnam University College
2023
Ningxia Medical University
2023
Catholic University of Korea
2023
Yeungnam University
2023
Objective Non-alcoholic fatty liver disease (NAFLD)-associated hepatocellular carcinoma (HCC) is an increasing healthcare burden worldwide. We examined the role of dietary cholesterol in driving NAFLD–HCC through modulating gut microbiota and its metabolites. Design High-fat/high-cholesterol (HFHC), high-fat/low-cholesterol or normal chow diet was fed to C57BL/6 male littermates for 14 months. Cholesterol-lowering drug atorvastatin administered HFHC-fed mice. Germ-free mice were transplanted...
Background & AimsAltered gut microbiota is implicated in development of colorectal cancer (CRC). Some intestinal bacteria have been reported to potentiate carcinogenesis by producing genotoxins, altering the immune response and microenvironment, activating oncogenic signaling pathways. We investigated whether stool from patients with CRC could directly induce mice.MethodsWe obtained stored samples participants a metagenome study performed Hong Kong. Conventional (male C57BL/6) mice were...
Stool samples from patients with colorectal cancer (CRC) have a higher abundance of Peptostreptococcus anaerobius than stool individuals without CRC, based on metagenome sequencing. We investigated whether P contributes to colon tumor formation in mice and its possible mechanisms carcinogenesis.We performed quantitative polymerase chain reaction analyses measure 112 255 biopsies CRC or advanced adenoma healthy (controls) undergoing colonoscopy examination at hospitals Hong Kong Beijing....
p38 mitogen-activated protein kinases are important inflammatory factors. p38α alteration has been implicated in both human and mouse disease models. Therefore, we aimed to characterize the cell type-specific role of non-alcoholic steatohepatitis (NASH).Human liver tissues were obtained from 27 patients with fatty (NAFLD) 20 control individuals. NASH was established compared between hepatocyte-specific knockout (p38αΔHep), macrophage-specific (p38αΔMΦ) wild-type (p38αfl/fl) mice fed high-fat...
Background & AimsGut microbial dysbiosis has pivotal involvement in colorectal cancer (CRC). However, the intratumoral microbiota and its association with CRC progression remain elusive. We aimed to determine community architecture within a neoplasia (CRC or adenoma) contribution carcinogenesis.MethodsWe collected 436 tissue biopsies from patients (n = 36) adenoma 32) (2–6 plus 2–5 adjacent normal tissues per individual). Microbial profiling was performed using 16S ribosomal RNA gene...
Non-alcoholic steatohepatitis (NASH) is the severe form of non-alcoholic fatty liver disease, and characterized by inflammation fat accumulation. Dietary interventions, such as fibre, have been shown to alleviate this metabolic disorder in mice via gut microbiota. Here, we investigated mechanistic role microbiota ameliorating NASH dietary fibre mice. Soluble inulin was found be more effective than insoluble cellulose suppress progression mice, reduced hepatic steatosis, necro-inflammation,...
Aberrant lipid metabolism is a hallmark of colorectal cancer (CRC). Squalene epoxidase (SQLE), rate-limiting enzyme in cholesterol biosynthesis, upregulated CRC. Here, we aim to determine oncogenic function SQLE and its interplay with gut microbiota promoting tumourigenesis.Paired adjacent normal tissues CRC from two cohorts were analysed (n=202). Colon-specific Sqle transgenic (Sqle tg) mice generated by crossing Rosa26-lsl-Sqle Cdx2-Cre mice. Stools collected for metagenomic metabolomic...
Recent studies have highlighted the role of gut microbiota and their metabolites in non-alcoholic fatty liver disease-associated hepatocellular carcinoma (NAFLD-HCC). We aimed to identify specific beneficial bacterial species that could be used prophylactically prevent NAFLD-HCC.
Objective Gut microbiota is a key player in dictating immunotherapy response. We aimed to explore the immunomodulatory effect of probiotic Lactobacillus gallinarum and its role improving anti-programmed cell death protein 1 (PD1) efficacy against colorectal cancer (CRC). Design The effects L. anti-PD1 response were assessed syngeneic mouse models azoxymethane/dextran sulfate sodium-induced CRC model. change immune landscape was identified by multicolour flow cytometry validated...
Streptococcus anginosus (S. anginosus) was enriched in the gastric mucosa of patients with cancer (GC). Here, we show that S. colonized mouse stomach and induced acute gastritis. infection spontaneously progressive chronic gastritis, parietal cell atrophy, mucinous metaplasia, dysplasia conventional mice, findings were confirmed germ-free mice. In addition, accelerated GC progression carcinogen-induced tumorigenesis YTN16 allografts. Consistently, disrupted barrier function, promoted...
Abstract Large-scale fecal shotgun metagenomic sequencing revealed the high abundance of Parvimonas micra in colorectal cancer (CRC) patients. We investigated role and clinical significance P. tumorigenesis. The was examined 309 samples 165 colon biopsy tissues CRC patients healthy subjects. significantly enriched from 128 compared to 181 subjects ( P < 0.0001); tissue biopsies 52 61 0.0001). Multivariate analysis showed that is an independent risk factor poor survival (Hazard Ratio:...
Background & AimsPien Tze Huang (PZH) is a well-established traditional medicine with beneficial effects against inflammation and cancer. We aimed to explore the chemopreventive effect of PZH in colorectal cancer (CRC) through modulating gut microbiota.MethodsCRC mouse models were established by azoxymethane plus dextran sulfate sodium treatment or Apcmin/+ mice treated without (270 mg/kg 540 mg/kg). Gut barrier function was determined means intestinal permeability assays transmission...
Gut probiotic depletion is associated with non-alcoholic fatty liver disease-associated hepatocellular carcinoma (NAFLD-HCC). Here, we investigated the prophylactic potential of Lactobacillus acidophilus against NAFLD-HCC.
Background & AimsCXC chemokine receptor 3 (CXCR3) is involved in virus-related chronic liver inflammation. However, the role of CXCR3 non-alcoholic steatohepatitis (NASH) remains unclear. We aimed to investigate NASH.MethodsHuman tissues were obtained from 24 fatty disease (NAFLD) patients and 20 control subjects. knockout (CXCR3−/−), obese db/db mice their wild-type (WT) littermates used both methionine-and-choline-deficient (MCD) diet high-fat high-carbohydrate high-cholesterol (HFHC)...
O-GlcNAc transferase (OGT) is a unique glycosyltransferase involved in metabolic reprogramming. We investigated the functional role of OGT non-alcoholic fatty liver disease-associated hepatocellular carcinoma (NAFLD-HCC).The biological function NAFLD-HCC was determined by gain- or loss- assays vitro and nude mice. target factors pathways were identified liquid chromatography-tandem mass spectrometry (LC-MS), promoter luciferase assay, DNA binding activity assay Western blot.OGT upregulated...
Traditional treatments for intermediate or advanced stage hepatocellular carcinoma (HCC) such as transarterial chemoembolization (TACE) and anti-angiogenesis therapies were developed to starve tumor blood supply. A new approach of normalizing structurally functionally abnormal vasculature is emerging. While TACE improves survival in selected patients, the resulting hypoxia stimulates proliferation, angiogenesis, treatment resistance metastasis, which limits its overall efficacy. Vessel...
Altered metabolites are important for the tumourigenicity of hepatocellular carcinoma (HCC). We performed integrative metabolomics analysis changes in portal venous blood and comparison with liver tissues stool samples HCC patients healthy donors.Serum (portal central vein), tissue (HCC tumour adjacent non-tumour, normal liver) were collected from 102 subjects (52 50 controls) discovery cohort; 100 (50 an independent validation cohort. Untargeted metabolomic profiling was using...
Abstract Appendectomy impacts the homeostasis of gut microbiome in patients. We aimed to study role appendectomy colorectal cancer (CRC) risk through causing microbial dysbiosis. Population-based longitudinal (cohort 1, n = 129,155) showed a 73.0% increase CRC among cases throughout 20 years follow-up (Adjusted sub-distribution hazard ratio (SHR) 1.73, 95% CI 1.49–2.01, P < 0.001). Shotgun metagenomic sequencing was performed on fecal samples from cohort 2 ( 314). Gut dysbiosis subjects...