A5071901988- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Gut microbiota and health
- Analytical Chemistry and Chromatography
- Cancer Research and Treatments
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Advanced Chemical Sensor Technologies
- Effects and risks of endocrine disrupting chemicals
- Microbial Metabolism and Applications
- Parkinson's Disease Mechanisms and Treatments
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Toxic Organic Pollutants Impact
- Microfluidic and Capillary Electrophoresis Applications
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Health, Environment, Cognitive Aging
- Neuroinflammation and Neurodegeneration Mechanisms
Chinese University of Hong Kong
2020-2025
Hong Kong Baptist University
2014-2024
Chinese University of Hong Kong, Shenzhen
2020-2024
Digestive Health Research Institute
2023
Shanghai Maritime University
2021
Jiangxi University of Science and Technology
2019
Roseburia intestinalis is a probiotic species that can suppress intestinal inflammation by producing metabolites. We aimed to study the role of R. in colorectal tumourigenesis and immunotherapy.R. abundance was evaluated stools patients with cancer (CRC) (n=444) healthy controls (n=575). The effects were studied ApcMin/+ or azoxymethane (AOM)-induced CRC mouse models, syngeneic xenograft models CT26 (microsatellite instability (MSI)-low) MC38 (MSI-high). change immune landscape multicolour...
Non-alcoholic steatohepatitis (NASH) is the severe form of non-alcoholic fatty liver disease, and characterized by inflammation fat accumulation. Dietary interventions, such as fibre, have been shown to alleviate this metabolic disorder in mice via gut microbiota. Here, we investigated mechanistic role microbiota ameliorating NASH dietary fibre mice. Soluble inulin was found be more effective than insoluble cellulose suppress progression mice, reduced hepatic steatosis, necro-inflammation,...
Recent studies have highlighted the role of gut microbiota and their metabolites in non-alcoholic fatty liver disease-associated hepatocellular carcinoma (NAFLD-HCC). We aimed to identify specific beneficial bacterial species that could be used prophylactically prevent NAFLD-HCC.
Objective Gut microbiota is a key player in dictating immunotherapy response. We aimed to explore the immunomodulatory effect of probiotic Lactobacillus gallinarum and its role improving anti-programmed cell death protein 1 (PD1) efficacy against colorectal cancer (CRC). Design The effects L. anti-PD1 response were assessed syngeneic mouse models azoxymethane/dextran sulfate sodium-induced CRC model. change immune landscape was identified by multicolour flow cytometry validated...
Gut probiotic depletion is associated with non-alcoholic fatty liver disease-associated hepatocellular carcinoma (NAFLD-HCC). Here, we investigated the prophylactic potential of Lactobacillus acidophilus against NAFLD-HCC.
Background & AimsMutant KRAS promotes glutaminolysis, a process that uses steps from the tricarboxylic cycle to convert glutamine α-ketoglutarate and other molecules via glutaminase SLC25A22. This results in inhibition of demethylases epigenetic alterations cells increase proliferation stem cell features. We investigated whether mutant KRAS-mediated glutaminolysis affects epigenomes activities colorectal cancer (CRC) cells.MethodsWe created ApcminKrasG12D mice with intestine-specific...
Abstract Therapeutic targeting of KRAS -mutant colorectal cancer (CRC) is an unmet need. Here, we show that Proprotein Convertase Subtilisin/Kexin type 9 (PSCK9) promotes APC/KRAS-mutant CRC and a therapeutic target. Using patient cohorts, isogenic cell lines transgenic mice, identify de novo cholesterol biosynthesis induced in APC / mutant CRC, accompanied by increased geranylgeranyl diphosphate (GGPP)─a metabolite necessary for activation. PCSK9 the top up-regulated cholesterol-related...
KRAS is an important tumor intrinsic factor driving immune suppression in colorectal cancer (CRC). In this study, we demonstrate that SLC25A22 underlies mutant KRAS-induced CRC. immunocompetent male mice and humanized models, knockout inhibits KRAS-mutant CRC growth with reduced myeloid derived suppressor cells (MDSC) but increased CD8+ T-cells, implying the reversion of KRAS-driven immunosuppression. Mechanistically, find plays a central role promoting asparagine, which binds activates SRC...
Squalene epoxidase (SQLE) promotes metabolic dysfunction-associated steatohepatitis-associated hepatocellular carcinoma (MASH-HCC), but its role in modulating the tumour immune microenvironment MASH-HCC remains unclear.
Peptostreptococcus stomatis (P. stomatis) is enriched in colorectal cancer (CRC), but its causality and translational implications CRC are unknown. Here, we show that P. accelerates colonic tumorigenesis Apc
Transmembrane-6 superfamily member 2 (TM6SF2) regulates hepatic fat metabolism and is associated with metabolic dysfunction-associated steatohepatitis (MASH). TM6SF2 genetic variants are steatotic liver disease. The pathogenesis of MASH involves factors gut microbiota alteration, yet the role host–microbe interactions in development remains unclear. Here, we discover that mice intestinal epithelial cell-specific knockout Tm6sf2 (Tm6sf2ΔIEC) develop MASH, accompanied by impaired barrier...
Backgrounds & AimsSqualene epoxidase (SQLE) is the rate-limiting enzyme for cholesterol biosynthesis. We elucidated functional significance, molecular mechanisms, and clinical impact of SQLE in nonalcoholic steatohepatitis (NASH).MethodsWe performed studies with hepatocyte-specific Sqle overexpression transgenic (Sqle tg) mice given high-fat high-cholesterol (HFHC) or methionine- choline-deficient (MCD) diet to induce NASH. downstream target carbonic anhydrase III (CA3) was identified using...
Identification of the direct molecular targets environmental pollutants is great importance for toxicity mechanism studies. Despite numerous studies have been conducted to investigate perfluorinated compounds (PFCs), their direct-binding protein which trigger downstream effects remain largely unknown. Herein, we present a systematic chemical proteomic study profile target proteins PFCs by taking PFOA as representative. Considering its electrophilicity, could preferentially bind reactive...
Due to the corruptions or noises that existed in real-world data sets, affinity graphs constructed by classical spectral clustering-based subspace clustering algorithms may not be able reveal intrinsic structures of sets faithfully. In this article, we reconsidered reconstruction problem and proposed idea "relation reconstruction." We pointed out a sample could represented neighborhood relation computed between its neighbors itself. The indicate true membership corresponding original...
Abstract The N6-methyladenosine (m6A) RNA-binding protein YTHDF1 is frequently overexpressed in colorectal cancer and drives chemotherapeutic resistance. To systematically identify druggable targets with high expression of YTHDF1, this study used a CRISPR/Cas9 screening strategy that revealed RUVBL1 RUVBL2 as putative targets. RUVBL1/2 were primary samples represented independent predictors poor patient prognosis. Functionally, loss preferentially impaired the growth YTHDF1-high cells,...
Recent discoveries of noncanonical RNA caps, such as nicotinamide adenine dinucleotide (NAD+) and 3′-dephospho-coenzyme A (dpCoA), have expanded our knowledge caps. Although dpCoA has been known to cap RNAs in various species, the identities its capped (dpCoA-RNAs) remained unknown. To fill this gap, we developed a method called tagSeq, which utilized thiol-reactive maleimide group label with tag serving 5′ barcode. The barcoded were isolated using complementary DNA strand prior direct...