A5057535865- Metabolomics and Mass Spectrometry Studies
- Effects and risks of endocrine disrupting chemicals
- Mass Spectrometry Techniques and Applications
- Toxic Organic Pollutants Impact
- Epigenetics and DNA Methylation
- Air Quality and Health Impacts
- Birth, Development, and Health
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Ion-surface interactions and analysis
- Bioinformatics and Genomic Networks
- Advanced Chemical Sensor Technologies
- Carcinogens and Genotoxicity Assessment
- Adipose Tissue and Metabolism
- Microplastics and Plastic Pollution
- Energy and Environment Impacts
- DNA and Nucleic Acid Chemistry
- RNA modifications and cancer
- Estrogen and related hormone effects
- Microbial Metabolic Engineering and Bioproduction
- Cancer, Hypoxia, and Metabolism
- Nanopore and Nanochannel Transport Studies
- Folate and B Vitamins Research
- Sphingolipid Metabolism and Signaling
- Glutathione Transferases and Polymorphisms
Chinese Academy of Sciences
2014-2024
Hong Kong Baptist University
2016-2024
Shenzhen Institutes of Advanced Technology
2019-2024
University of Chinese Academy of Sciences
2023-2024
Yangzhou University
2024
Shenzhen Second People's Hospital
2024
Shenzhen University Health Science Center
2024
Shanghai Pulmonary Hospital
2020
Research Center for Eco-Environmental Sciences
2011-2018
DNA hydroxymethylation and its mediated demethylation are critical for multiple cellular processes, example, nuclear reprogramming, embryonic development, many diseases. Here, we demonstrate that a vital nutrient ascorbic acid (AA), or vitamin C (Vc), can directly enhance the catalytic activity of Tet dioxygenases oxidation 5-methylcytosine (5mC). As evidenced by changes in intrinsic fluorescence Tet2 protein caused AA oxidation-resistant derivatives, further show uniquely interact with...
DNA methylation has been proven to be a critical epigenetic mark important for various cellular processes. Here, we report that redox-active quinones, ubiquitous class of chemicals found in natural products, cancer therapeutics and environment, stimulate the conversion 5mC 5hmC vivo, increase 5751 genes cells. is associated with significantly altered gene expression 3414 genes. Interestingly, quinone-treated cells, labile iron-sensitive protein ferritin light chain showed significant at both...
With the continuous exposure of environmental pollutants in organisms, determination abundance variation and spatial distribution lipids might expand our understanding toxicological mechanisms occurring kidney. Herein, an integrated method involving mass spectrometry (MS)-based lipidomics matrix-assisted laser desorption/ionization-MS imaging (MALDI-MSI) was developed for study nephrotoxicity mice exposed to 10 100 μg bisphenol S (BPS)/kg body weight/day. The BPS remarkable perturbed...
Abstract Emerging tactile sensing devices mimic biological functions of human mechanoreception. By introducing the feature optical transparency, it can lead to a combined capacities and visual intelligence into single system. Yet, is difficult realize ultrahigh level transparency device sensitivity in structure, for widely used methods improvement, such as elevating interfacial roughness, may further reduce transparency. utilizing transparent ionic material with tunable surface topologies,...
Spatiotemporal heterogeneity of tumors provides an escape mechanism for breast cancer cells, which can obstruct the investigation tumor progression. While molecular profiling obtained from mass spectrometry imaging (MSI) is rich in biochemical information, it lacks capacity vivo analysis. Ultrasound diagnosis has a high diagnostic accuracy but low chemical specificity. Here, we describe noninvasive ultrasound elastography (UE)-guided MSI strategy (UEg-MSI) that integrates physical and...
Acrolein (Acr), a ubiquitous environmental pollutant, can react directly with genomic DNA to form mutagenic adducts without undergoing metabolic activation. To sensitively and accurately quantify Acr-DNA (including structural isomers stereoisomers) in human leukocytes, we developed an enhanced stable isotope dilution ultrahigh performance liquid chromatography (UHPLC)-tandem mass spectrometry (MS/MS) method using ammonium bicarbonate (NH4HCO3), which is thermally unstable degrades readily...
Rationale Bisphenols, such as bisphenol A (BPA) and S (BPS), are widely used in industrial products, although they have been demonstrated to be environmental contaminants with toxicity. However, few studies on the mass spectrometric fragmentation pathway of these compounds reported using high-resolution spectrometry (HRMS). Methods The MS/MS fragmentations nine bisphenols, together several corresponding isotope-labeled compounds, were studied by Orbitrap MS electrospray ionization (ESI)...
Using advanced molecular imaging techniques, we can acquire the primary disturbance of spatial and temporal information based on histological variations, which has potential to differentiate intra-/extraheterogeneity predict disease progression or metastasis status in biological systems. Most chemical techniques depend "inherent properties" visualize target compounds their interactions from different forms samples, such as elemental bond information, mass-to-charge ratio (m/z), fragment, ion...
Research on metabolic heterogeneity provides an important basis for the study of molecular mechanism a disease and personalized treatment. The screening metabolism-related sub-regions that affect development is essential more focused exploration progress aberrant phenotypes, even carcinogenesis metastasis. mass spectrometry imaging (MSI) technique has distinct advantages to reveal organism based in situ profiles. challenge heterogeneous analysis been perform objective identification among...
Mass spectrometry imaging (MSI) could provide vast amounts of data at the temporal–spatial scale in heterogeneous biological specimens, which challenges us to segment accurately suborgans/microregions from complex MSI data. Several pipelines had been proposed for spatial segmentation past decade. More importantly, filtering was found be an efficient procedure improve outcomes pipelines. It is not clear, however, how affects segmentation. An improved pipeline established by elaborating...
As a bisphenol A (BPA) alternative, F (BPF) has been detected in various products, such as paper personal care and food. More importantly, the toxicity of BPF remains underexplored. We reported an integrated method to study immunotoxic potentials underlying mechanisms on cell apoptosis, macrophage polarization, reactive oxygen species generation, expression secretion immune-related cytokines, reprogramming lipid signaling. serious BPA, induced apoptosis macrophages. The was by activating...
Extracellular vesicles (EVs) are actively secreted by mammalian cells. They increasingly recognized as promising circulating biomarkers of disease progression. Matrix-assisted laser desorption/ionization-time flight mass spectrometry (MALDI-TOF MS) is currently one the most powerful techniques for rapid analysis biological samples, especially discovering diagnosis and prognosis. It unclear what cell culture medium components EV isolation methods suitable MALDI-TOF MS analysis. Using a human...
Spatial segmentation is a critical procedure in mass spectrometry imaging (MSI)-based biochemical analysis. However, the commonly used unsupervised MSI methods may lead to inappropriate results as data characterized by high dimensionality and low signal-to-noise ratio. This process can be improved incorporation of precise prior knowledge, which hard obtain most cases. In this study, we show that partial or coarse knowledge from different sources such reference images biological also help...
The gene- or fragment-specific detection of newly recognized deoxyribonucleic acid (DNA) base 5-hydroxymethylcytosine (5hmC) will provide insights into its critical functions in development and diseases, is also important for screening 5hmC-rich genes as an indicator epigenetic states, pathogenic processes pharmacological responses. Current analytical technologies gene-specific 5hmC are heavily dependent on glucosylated 5hmC-resistant restriction endonuclease cleavage. Here, we find that...