A5002405933- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- Per- and polyfluoroalkyl substances research
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- Tryptophan and brain disorders
- Toxic Organic Pollutants Impact
- Folate and B Vitamins Research
- Cancer-related molecular mechanisms research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Digital Media and Visual Art
- CRISPR and Genetic Engineering
- Diet and metabolism studies
- Advanced biosensing and bioanalysis techniques
- Barrier Structure and Function Studies
- Click Chemistry and Applications
University of Hong Kong
2024
State Key Laboratory of Synthetic Chemistry
2024
Hong Kong Baptist University
2018-2023
Tsinghua University
2016
University Town of Shenzhen
2016
Triclosan (TCS), an extensively used antimicrobial agent, has raised considerable concern due to its hepatocarcinogenic potential. However, previous hepatotoxicity studies primarily focused on the activation of specific intracellular receptors, underlying mechanisms still warrant further investigation at metabolic level. Herein, we applied metabolomics in combination with lipidomics unveil TCS-related responses human normal and cancerous hepatocytes. Endogenous exogenous metabolites were...
The 5′ end of a eukaryotic mRNA transcript generally has 7-methylguanosine (m 7 G) cap that protects from degradation and mediates almost all other aspects gene expression. Some RNAs in Escherichia coli , yeast, mammals were recently found to contain an NAD + cap. Here, we report the development method tagSeq for transcriptome-wide identification quantification -capped (NAD-RNAs). uses enzymatic reaction then click chemistry label NAD-RNAs with synthetic RNA tag. tagged molecules can be...
Identification of the direct molecular targets environmental pollutants is great importance for toxicity mechanism studies. Despite numerous studies have been conducted to investigate perfluorinated compounds (PFCs), their direct-binding protein which trigger downstream effects remain largely unknown. Herein, we present a systematic chemical proteomic study profile target proteins PFCs by taking PFOA as representative. Considering its electrophilicity, could preferentially bind reactive...
Recent findings regarding nicotinamide adenine dinucleotide (NAD+)-capped RNAs (NAD-RNAs) indicate that prokaryotes and eukaryotes employ noncanonical RNA capping to regulate gene expression. Two methods for transcriptome-wide analysis of NAD-RNAs, NAD captureSeq tagSeq, are based on copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry label NAD-RNAs. However, copper ions can fragment/degrade RNA, interfering with the analyses. Here we report development tagSeq II, which uses...
A rational strategy was introduced for the synthesis of a novel dual functionalized metal–organic framework nanocomposite (AuGC/ZIF-8) with ultra-hydrophilicity to enhance glycopeptide enrichment.
Recent discoveries of noncanonical RNA caps, such as nicotinamide adenine dinucleotide (NAD+) and 3′-dephospho-coenzyme A (dpCoA), have expanded our knowledge caps. Although dpCoA has been known to cap RNAs in various species, the identities its capped (dpCoA-RNAs) remained unknown. To fill this gap, we developed a method called tagSeq, which utilized thiol-reactive maleimide group label with tag serving 5′ barcode. The barcoded were isolated using complementary DNA strand prior direct...
A silver( i ) complex AgTU exerts anticancer activities by releasing Ag + ions that target and impair the 19S proteasomal complex, resulting in accumulation of ubiquitinated, misfolded proteins.
Post-translational modification of proteins can form electrophilic cofactors that serve as a catalytic center. The derived greatly expand protein activities and functions. However, there are few studies concerning how to profile the electrophiles in bacteria. Herein, we utilized clickable probe called propargyl hydrazine protein-derived Escherichia coli (E. coli) cells. Since mostly carbonyl groups, hydrazine-based specifically react with Schiff base. labeled were then pulled down for mass...
ADVERTISEMENT RETURN TO ISSUEPREVCommentNEXTResponse to the Comment on "DpCoA tagSeq: Barcoding dpCoA-Capped RNA for Direct Nanopore Sequencing via Maleimide-Thiol Reaction"Xiaojian ShaoXiaojian ShaoMore by Xiaojian Shao, Hailei ZhangHailei ZhangMore Zhang, Zhou ZhuZhou ZhuMore Zhu, Fenfen JiFenfen JiMore Ji, Zhao HeZhao HeMore He, Zhu Yang*Zhu YangMore Yanghttps://orcid.org/0000-0001-5934-1617, Yiji Xia*Yiji XiaMore Xia, and Zongwei Cai*Zongwei CaiMore...
Introduction: A previous epigenome-wide association study causally linked DNA methylation (DNAm) at NFATC2IP gene (cg26663590) on body mass index (BMI). However, whether DNAm is associated with cardiovascular health (CVH) unknown. Methods: We included 408 participants from the POUNDs Lost trial. was profiled by sequencing method baseline. Regional defined as average level over ± 250 bp of cg26663590. calculated clinical CVH score baseline, 6 months, and 2 years, combining BMI, blood...
<p dir="ltr">Objective: To examine whether participants with different levels of diabetes-related DNA methylation at ABCG1 might respond differently to dietary weight-loss interventions long-term changes in adiposity and body fat distribution. </p><p dir="ltr">Research Design Methods: The current study included overweight/obese from the POUNDS lost trial. Blood regional were profiled by high-resolution methylC-capture sequencing baseline among 673 participants, which 598...
<p dir="ltr">Objective: To examine whether participants with different levels of diabetes-related DNA methylation at ABCG1 might respond differently to dietary weight-loss interventions long-term changes in adiposity and body fat distribution. </p><p dir="ltr">Research Design Methods: The current study included overweight/obese from the POUNDS lost trial. Blood regional were profiled by high-resolution methylC-capture sequencing baseline among 673 participants, which 598...
Background: Epigenome-wide association studies identified DNA methylation (DNAm) at several CpG sites of the carnitine palmitoyltransferase-1A (CPT1A) gene to be associated with triglycerides (TG) . However, little is known about whether pre-treatment DNAm level CPT1A changes in TG response weight-loss diet interventions. Methods: We included 673 white participants overweight or obesity, who were randomly assigned 1 4 diets varying macronutrients components. Blood levels profiled by a...