A5076967518- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Diet and metabolism studies
- Animal Genetics and Reproduction
- Cardiac Imaging and Diagnostics
- Cardiovascular Disease and Adiposity
- Blood Coagulation and Thrombosis Mechanisms
- Regulation of Appetite and Obesity
- Coronary Interventions and Diagnostics
- Liver Disease Diagnosis and Treatment
- Nuclear Receptors and Signaling
- Diet, Metabolism, and Disease
- Peptidase Inhibition and Analysis
- Cardiac electrophysiology and arrhythmias
- Lipid metabolism and biosynthesis
- Advanced MRI Techniques and Applications
Kobe University
2013-2025
University of Michigan
2009-2025
Michigan Center for Translational Pathology
2020-2022
University of Yamanashi
2006-2020
Central Institute for Experimental Animals
2018
Obayashi (Japan)
2013
Brigham and Women's Hospital
2013
Shiga University of Medical Science
2013
Harvard University
2013
University of Miyazaki
2006-2012
A glycine- and leucine-based tripeptide improves symptoms in a mouse model of nonalcoholic fatty liver disease, which lacks approved treatments.
Lipidomics, the mass spectrometry-based comprehensive analysis of lipids, has attracted attention as an analytical approach to provide novel insight into lipid metabolism and search for biomarkers. However, ideal method both quantitative lipids not been fully developed. Here, we have proposed a practical methodology widely targeted lipidome using supercritical fluid chromatography fast-scanning triple-quadrupole spectrometry (SFC/QqQMS) theoretically calculated multiple reaction monitoring...
Macrophage metalloelastase (matrix metalloproteinase [MMP]-12) is upregulated in atherosclerotic lesions and aneurysm; thus, increased MMP-12 activity may play an important role the pathogenesis of atherosclerosis. However, pathological roles initiation progression atherosclerosis have not been defined.We compared susceptibility transgenic (Tg) rabbits to cholesterol-rich diet-induced with that non-Tg littermate rabbits. The were maintained at either relatively lower levels...
Vascular endothelial growth factor (VEGF) is an important mediator in maintaining normal kidney functions. In addition, several lines of evidence suggest that upregulation VEGF glomeruli may be associated with or cause renal dysfunction such as diabetic nephropathy. For elucidation the pathologic consequences high levels glomeruli, transgenic (Tg) rabbits express human VEGF(165) isoform both and liver under control alpha-1-antitrypsin promoter were generated characterized. With use...
Although there is a statistically significant association between modestly raised baseline plasma C-reactive protein (CRP) values and future cardiovascular events, the debate still unsettled in regard to whether CRP plays causal role pathogenesis of atherosclerosis.We generated 2 lines transgenic (Tg) rabbits expressing human (hCRP). The levels hCRP hCRP-Tg-1 hCRP-Tg-2 were 0.4+/-0.13 (n=14) 57.8+/-20.6 mg/L (n=12), respectively. In addition, isolated from Tg rabbit exhibited ability...
Apolipoprotein (apo) A-II is the second major apo of high-density lipoproteins, yet its pathophysiological roles in development atherosclerosis remain unknown. We aimed to examine whether plays any role atherogenesis and, if so, elucidate mechanism involved.We compared susceptibility human transgenic (Tg) rabbits cholesterol diet-induced with non-Tg littermate rabbits. Tg developed significantly less aortic and coronary than their littermates, while total plasma levels were similar....
Abstract Matrix metalloproteinase‐9 (MMP‐9), or gelatinase B, has been hypothesized to be involved in the progression of atherosclerosis. In arterial wall, accumulated macrophages secrete considerable amounts MMP‐9 but its pathophysiological functions atherosclerosis have not fully elucidated. To examine hypothesis that macrophage‐derived may affect atherosclerosis, we created transgenic (Tg) rabbits overexpress rabbit gene under control scavenger receptor A enhancer/promoter and examined...
Summary Type 2 diabetes is a major risk factor of the development atherosclerosis in humans. However, studies examining mechanisms underlying diabetes‐accelerated have been limited by lack suitable humanoid animal models. Pigs cardiovascular system that very similar to humans and useful as model for human physiology pathophysiology. In this study, we established new miniature pig studying dyslipidaemia diabetes. Chinese Guizhou minipigs were fed normal control diet or high‐fat/high‐sucrose...
Individuals with insulin resistance and resulting impaired glucose tolerance along type 2 diabetes showed an increased prevalence of atherosclerosis. Our aim in this study was to address whether diet-induced plays any roles the development aortic coronary atherosclerosis hyperlipidemic rabbits.We fed Watanabe heritable (WHHL) rabbits a high-fructose high-fat diet (HFFD) restricted normal calories compared lesions both those control WHHL chow diet.HFFD-fed accompanied by elevated plasma lipid...
Abstract Objective Increased enzymatic activity of matrix metalloproteinases (MMPs) may promote the progression rheumatoid arthritis (RA). We undertook this study to investigate expression and localization human macrophage elastase (MMP‐12) in synovial tissue from RA patients compare MMP‐12 levels fluid with corresponding osteoarthritis (OA). Methods obtained tissues 23 29 OA analyzed using immunohistochemistry, Western Northern blotting analyses, zymography. Furthermore, we quantified by...
Lipoprotein lipase (LPL) is the rate-limiting enzyme for hydrolysis of triglyceride-rich lipoproteins and plays a critical role in lipoprotein free fatty acid metabolism. Genetic manipulation LPL may be beneficial treatment hypertriglyceridemias, but it unknown whether increased activity effective lowering plasma cholesterol improving insulin resistance familial hypercholesterolemic patients. To test hypothesis that stimulation expression used as an adjunctive therapy homozygous...
This study tested the hypothesis that vasospasm can trigger coronary plaque injury and acute ischemic myocardial damage.Myocardial infarction-prone strain of Watanabe heritable hyperlipidemic rabbits received an intravenous bolus ergonovine maleate (0.45 µmol/kg) during infusion norepinephrine (12 nmol/kg per minute) to provoke spasm in vivo. After this treatment, angiography demonstrated vasospasm, ECG showed abnormalities (ST depression/elevation T-wave inversion) 77% animals (23/30)....
Apolipoprotein AII (apoAII) is the second major apolipoprotein in high-density lipoprotein (HDL). However, physiological functions of apoAII metabolism have not been fully elucidated.We generated human transgenic (Tg) rabbits, a species that normally does an endogenous gene. Plasma levels Tg rabbits were approximately 30 mg/dL, similar to plasma healthy humans. The expression resulted increased triglycerides, total cholesterol, and phospholipids accompanied by marked reduction...
Abstract The rabbit ( Oryctolagus cuniculus ) is an important experimental animal for studying human diseases, such as hypercholesterolemia and atherosclerosis. Despite this, genetic information RNA expression profiling of laboratory rabbits are lacking. Here, we characterized the whole-genome variants three breeds most popular rabbits, New Zealand White (NZW), Japanese (JW) Watanabe heritable hyperlipidemic (WHHL) rabbits. Although diversity WHHL was relatively low, they accumulated a large...
Lipoproteins, which are synthesized in hepatic cells and secreted into the blood, play an important role lipid transport. The quality of lipoproteins, including small dense low-density lipoproteins cholesterol efflux capacity high-density influences cardiovascular outcomes, although relationship between changes lipoprotein remains unclear. This study characterized molecules apolipoproteins particles during development coronary atherosclerosis myocardial infarction-prone rabbits. Using...
Probucol is a lipid-lowering drug that often prescribed for the treatment of familial hypercholesterolemia. However, it not known whether probucol can change lesion quality atherosclerosis.We examined this possibility using WHHL rabbits, model human Three-month-old male rabbits were treated with either probucol(85 mg/kg/day) or atorvastatin(6 16 weeks, and their plasma lipid levels atherosclerotic lesions compared those control group.We found reduced cholesterol levels, but less remarkably...