A5100701206- Photoacoustic and Ultrasonic Imaging
- Corneal Surgery and Treatments
- Ultrasound and Hyperthermia Applications
- Corneal surgery and disorders
- Retinal Diseases and Treatments
- Intraocular Surgery and Lenses
- Retinal and Optic Conditions
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Optical Coherence Tomography Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Retinal and Macular Surgery
- Nanoplatforms for cancer theranostics
- Retinal Imaging and Analysis
- Nitric Oxide and Endothelin Effects
- Ocular Surface and Contact Lens
- vaccines and immunoinformatics approaches
- Glaucoma and retinal disorders
- Psoriasis: Treatment and Pathogenesis
- Ocular Diseases and Behçet’s Syndrome
- Thermography and Photoacoustic Techniques
- Cerebral Venous Sinus Thrombosis
- Ultrasound and Cavitation Phenomena
- Advanced Fluorescence Microscopy Techniques
- Connexins and lens biology
Southwest Medical University
2025
Tongji University
2017-2024
Kunming University of Science and Technology
2023-2024
Shanghai Skin Disease Hospital
2021-2024
Fudan University
2024
University of Michigan
2014-2022
Institute of Acoustics
2018-2022
China Medical University
2016-2021
Michigan United
2018-2021
Fourth Affiliated Hospital of China Medical University
2016-2021
Therapies targeting VEGF have proven only modestly effective for the treatment of proliferative sickle cell retinopathy (PSR), leading cause blindness in patients with disease. Here, we shift our attention upstream from genes that promote retinal neovascularization (NV) to transcription factors regulate their expression. We demonstrated increased expression HIF-1α and HIF-2α ischemic inner retina PSR eyes. Although both HIFs participated promoting by hypoxic Müller cells, HIF-1 alone was...
Combination immunotherapy is a promising strategy to remove the inhibitory effect of tumor microenvironment on immune effector cells, improving efficacy checkpoint inhibitor treatment in bladder cancer. However, it challenging deliver multiple drugs tissue effectively and simultaneously ensure optimal therapeutic effects. Macrophage-derived exosome-mimetic nanovesicles (EMVs) were designed validated as nanoplatform for coloading delivery CD73 (AB680) monoclonal antibody programmed cell death...
ABSTRACT Our previous studies have identified mitsugumin 53 (MG53) as a novel regulator for angiogenesis by directly entering endothelial cells and modulating focal adhesion kinase (FAK) activation, but little is known about how rhMG53 taken up mediates cell movement. In the present study, we demonstrated that knockdown of caveolin‐1 clathrin inhibitor, pitstop‐2, both significantly reduced entry into cells, indicating caveolae‐dependent clathrin‐dependent endocytosis during this process....
The goal of this study is to further develop a multi-modality eye imaging system and evaluate its feasibility acquiring images different modalities simultaneously. An integrated multimodality combining spectral-domain optical coherence tomography (SD-OCT), photoacoustic microscopy (PAM), fluorescence (FM) was developed, performance for validated on multiple clinically-relevant retinal disease models in vivo rabbits. OCT allows visualization the anatomic layers with high axial resolution. PAM...
A new portable gas phase nitric oxide (NO) generator is described for potential applications in inhaled NO (INO) therapy and during cardiopulmonary bypass (CPB) surgery. In this system, produced at the surface of a large-area mesh working electrode by electrochemical reduction nitrite ions presence soluble copper(II)-ligand electron transfer mediator complex. The generated then transported into either direct purging with nitrogen/air or via circulating electrolyte/nitrite solution through...
In this study, high-throughput sequencing and metabolomics analysis were conducted to analyze the microbial metabolites of dry-cured Sanchuan ham, Laowo Nuodeng Heqing ham that have fermented for two years produced from western Yunnan China. Results showed at genus level, dominant bacteria in four types Halomonas Staphylococcus, while fungi Aspergillus Yamadazyma. A total 422 different identified mainly amino acids, peptides, fatty their structural analogs, which involved pantothenate...
Background Tuberculosis (TB) is a disease caused by the chronic and continuous infection of pathogen Mycobacterium tuberculosis (M. tuberculosis). M. an intracellular bacterial eliminated mainly through CD4+ effector Th cells. induces regulatory T lymphocytes (Tregs) that mediate immune suppression cell-to-cell contact or secreting cytokines such as transforming growth factor-β (TGF-β). To understand role T-cells in pathogenesis TB, we have measured vivo frequency associated cytokine...
Transrectal ultrasound (TRUS)-guided biopsy is the standard procedure for evaluating presence and aggressiveness of prostate cancer. TRUS involves tissue removal, suffers from low core yield as well high false negative rate. A less invasive more accurate diagnostic cancer therefore highly desired. Combining optical sensitivity ultrasonic resolution to resolve spatial distribution major molecular components in tissue, photoacoustic (PA) technology could be an alternative approach diagnosis...
Pathologic microvasculature plays a crucial role in innumerable diseases causing death and major organ impairment. A clinical challenge is the development of selective therapies to remove these diseased microvessels without damaging surrounding tissue. This report describes our novel photo-mediated ultrasound therapy (PUT) technology for precisely removing choroidal blood vessels eye. PUT selectively removes by concurrently applying nanosecond laser pulses with bursts. In experiments on...
We demonstrated the ultrasound modulated droplet lasers, in which laser intensity from whispering gallery mode (WGM) of oil droplets can be reversibly enhanced up to 20-fold when pressure is beyond a certain threshold. The lasing enhancement was investigated with various frequencies and pressures. Furthermore, modulation output achieved by controlling pressure, duty cycle, frequency bursts. Its potential application explored via study on human whole blood vessel phantom. A theoretical...
Photo-mediated ultrasound therapy (PUT) is a novel, non-invasive, agent-free, highly selective, and precise anti-vascular technique. PUT removes microvessels through promoting cavitation activity precisely in targeted by applying synchronized nanosecond laser pulses bursts. The synchronization between critical to the outcome of PUT.Through theoretical simulation experimental study, effect bursts on during evaluated.By using model, we found that was enhanced when were such produced...
Background The purpose of this study was to evaluate the performance BACTEC MGIT 960 (M960) system compared with proportion method (PM) on Löwenstein-Jensen (L-J) medium in a peripheral laboratory China for testing Mycobacterium tuberculosis (MTB) susceptibility streptomycin (SM), isoniazid (INH) rifampicin (RIF) and ethambutol (EMB) combination known as SIRE. Methods 205 clinical isolates MTB SM, INH, RIF EMB performed M960 system. drugs were tested at following concentrations: 1.0 µg/ml...
Photo-mediated ultrasound therapy (PUT) is a non-invasive, agent-free technique to shut down microvessels with high precision by promoting cavitation activity precisely in the targeted microvessels. PUT based on photoacoustic (PA) generated through concurrently applied nanosecond laser pulses and bursts. In this study, PA model employed understand enhanced during PUT, full consideration of optical absorption blood vessels. Bubble size evolution cylindrically-shaped absorbers (vessels) due...
Abstract Objectives Retinal neovascularization (RNV) is the growth of abnormal microvessels on retinal surface and into vitreous, which can lead to severe vision loss. By combining relatively low‐intensity ultrasound nanosecond‐pulse‐duration laser, we developed a novel treatment method, namely photo‐mediated therapy (PUT), holds potential remove RNV with minimal or no damage adjacent tissues. Methods was created in both albino pigmented rabbits ( n = 10) through single intravitreal...
Abstract Background Tuberculosis is one of the most infectious diseases in world. Molecular typing methods such as spoligotyping, and VNTR (variable number tandem repeats), IS 6110 NTF region LSP (large sequence polymorphisms) analysis are generally useful tools for resolution various issues related to classical epidemiology Mycobacterium tuberculosis ( M. ). Methods To determine transmission characteristics strains isolated Beijing, China, their genetic relationships, especially those among...
Photo-mediated ultrasound therapy (PUT) is a novel, noninvasive antimicrovascular approach that can treat neovascularization with high precision. We developed photoacoustic (PA) sensing (PAS) system for PUT and achieved real-time PAS-guided PUT. Experiments performed on chicken yolk sac membrane model demonstrated PAS could monitor the treatment effect in microvessel during Vessel shrinkage induced decrease PA signal amplitude, while vessel rupture an abrupt increase amplitude. The...
Psoriasis is an inflammatory skin disease mediated by the immune system and characterized ring, also known as epithelial microenvironment (EIME). The interaction between tissue of has a crucial role in cycle psoriasis. Although formation new blood vessels lesions provides energy support for proliferation epidermal keratinocytes, angiogenesis psoriasis not been extensively studied. Vascular endothelial growth factor A (VEGFA) key regulator that important development VEGFA promotes directly...
Purpose: Corneal neovascularization (CNV) is the invasion of new blood vessels into avascular cornea, leading to reduced corneal transparency and visual acuity, impaired vision, even blindness. Current treatment options for CNV are limited. We developed a novel method, termed photo-mediated ultrasound therapy (PUT), that combines laser ultrasound, we tested its feasibility treating in rabbit model. Methods: A suture-induced model was established New Zealand White rabbits, which were randomly...
Most schistosome-endemic areas in China are characterized by low-intensity infections that independent of prevalence. To establish an effective diagnostic method, we developed a magnetic affinity enzyme-linked immunoassay based on soluble egg antigens (SEA-MEIA) for diagnosing schistosomiasis persons with infection Schistosoma japonicum comparing it conventional immunosorbent assay (ELISA). Our results showed the SEA-MEIA had higher sensitivity and greater precision diagnosis S. than ELISA....
Corneal neovascularization (NV) is a sight-threatening condition often associated with infection, inflammation, prolonged contact lens use, corneal burns, and acute graft rejection. Macrophages recruited to the cornea release nitric oxide (NO) superoxide anion (O2(-)), which react together form highly toxic molecule peroxynitrite (ONOO(-)). The role of ONOO(-) in upregulating multiple angiogenic factors cultured human limbal epithelial (HCLE) cells was investigated.Human were incubated 500...
To explore the molecular mechanisms in lens development and pathogenesis of Peters anomaly Smad4 defective mice.Le-Cre transgenic mouse line was employed to inactivate surface ectoderm selectively. Pathological techniques were used reveal morphological changes anterior segment eye. Immunohistochemical staining observe expression E-cadherin, N-cadherin α-SMA mice control at embryonic (E) day 16.5. Real-time quantitative polymerase chain reaction (qPCR) performed detect Snail, Zeb1, Zeb2...
Background and Objectives We have developed a novel anti‐vascular technique, termed photo‐mediated ultrasound therapy (PUT), which utilizes nanosecond duration laser pulses synchronized with bursts to remove the microvasculature through cavitation. The objective of current study is explore potential PUT in removing subcutaneous microvessels. Study Design/Materials Methods auricular blood vessels two New Zealand white rabbits were treated by peak negative pressure 0.45 MPa at 0.5 MHz, fluence...
This report describes a novel therapeutic technique called photo-mediated ultrasound therapy (PUT). PUT applies synchronized short pulse duration (nanosecond) laser and burst on targeted tissue, offering high-precision localized treatment. is based controlled induction promotion of micro-cavitation activity in the target tissue. able to safely effectively treat retinal neovascularization rabbits with persistent nonperfusion up 4 weeks after choroidal vasculature.Clinical Relevance- can...
Host immune pressure and associated evasion of pathogenic bacteria are key features host-pathogen co-evolution. A previous study showed that human T-cell epitopes Mycobacterium tuberculosis evolutionarily hyperconserved thus it was deduced M. lacks antigenic variation evasion. Here, we selected 173 clinical complex (MTBC) isolates from China, amplified the genes encoding Rv2945c Rv0309, compared sequences. The results genetic diversity existed in these two among MTBC strains single...