A5023582215- Angiogenesis and VEGF in Cancer
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Metabolism, Diabetes, and Cancer
- Advanced Glycation End Products research
- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Blood Coagulation and Thrombosis Mechanisms
- Acute Ischemic Stroke Management
- Venous Thromboembolism Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Galectins and Cancer Biology
- Lymphatic System and Diseases
- Medicinal Plants and Neuroprotection
- Periodontal Regeneration and Treatments
- Cancer, Hypoxia, and Metabolism
- Platelet Disorders and Treatments
- Genomics, phytochemicals, and oxidative stress
- Signaling Pathways in Disease
- Peripheral Artery Disease Management
- Cellular Mechanics and Interactions
- Polysaccharides and Plant Cell Walls
- Lung Cancer Treatments and Mutations
- Pressure Ulcer Prevention and Management
Southwest Medical University
2014-2025
West China Hospital of Sichuan University
2024
Sichuan University
2024
First Affiliated Hospital of Sichuan Medical University
2012-2023
Affiliated Hospital of Southwest Medical University
2023
Hangzhou Medical College
2015
Harry S. Truman Memorial Veterans' Hospital
2014
University of Missouri
2014
University of Michigan
2014
Lac Courte Oreilles Ojibwa Community College
2014
Methylglyoxal (MGO) is an active metabolite of glucose and plays a prominent role in the pathogenesis diabetic vascular complications, including endothelial cell apoptosis induced by oxidative stress. Metformin (MET), widely prescribed antidiabetic agent, appears to reduce excessive reactive oxygen species (ROS) generation limit apoptosis. However, molecular mechanisms underlying this process are still not fully elucidated. We reported here that MET prevents MGO-induced suppressing stress...
Plasminogen activator inhibitor-1 (PAI-1) regulates angiogenesis via effects on extracellular matrix proteolysis and cell adhesion. However, no previous study has implicated PAI-1 in controlling vascular endothelial growth factor (VEGF) signaling. We tested the hypothesis that downregulates VEGF receptor-2 (VEGFR-2) activation by inhibiting a vitronectin-dependent cooperative binding interaction between VEGFR-2 αVβ3.
An increased incidence of venous thromboembolism (VTE) is associated with anti-vascular endothelial growth factor (VEGF) treatment in cancer. However, the mechanism underlying this effect remains elusive. In study, we examined bevacizumab, a humanized monoclonal antibody against VEGF-A, on VTE murine xenograft A549 cell tumor model.Inferior vena cava stenosis model and FeCl3-induced saphenous vein thrombosis were performed mouse models human lung adenocarcinoma.We found that bevacizumab...
ABSTRACT Our previous studies have identified mitsugumin 53 (MG53) as a novel regulator for angiogenesis by directly entering endothelial cells and modulating focal adhesion kinase (FAK) activation, but little is known about how rhMG53 taken up mediates cell movement. In the present study, we demonstrated that knockdown of caveolin‐1 clathrin inhibitor, pitstop‐2, both significantly reduced entry into cells, indicating caveolae‐dependent clathrin‐dependent endocytosis during this process....
Methylglyoxal (MGO), an active metabolite of glucose, has been reported to induce vascular cell apoptosis in diabetic complication. Polydatin (PD), a small natural compound from Polygonum cuspidatum, number biological functions, such as antioxidative, anti-inflammatory, and nephroprotective properties. However, the protective effects PD on MGO-induced endothelial cells remain be elucidated. In this study, human umbilical vein (HUVECs) were used explore possible mechanism involved. HUVECs...
Background Cross-talk between integrins and cadherins regulates cell function. We tested the hypothesis that vitronectin (VN), a multi-functional adhesion molecule present in extracellular matrix plasma, vascular permeability via effects on VE-cadherin, critical regulator of endothelial (EC) adhesion. Methodology/Principal Findings Addition multimeric VN (mult VN) significantly increased VE-cadherin internalization human umbilical vein EC (HUVEC) monolayers. This effect was blocked by...
Abstract Mitochondrial pyruvate carrier 1 (MPC1), a key factor that controls transportation in the mitochondria, is known to be frequently dysregulated tumor initiation and progression. However, clinical relevance potential molecular mechanisms of MPC1 lung adenocarcinoma (LAC) progression remain illustrated. Herein, was lowly expressed LAC tissues significantly associated with favorable survival patients LAC. Functionally, markedly suppressed stemness, invasion, migration vitro spreading...
The present study aimed to investigate the effects of asiaticoside (AS) on pathology and associated mechanisms β-amyloid (Aβ)-induced Alzheimer's disease (AD) in rats. An AD rat model was established by lateral intracerebroventricular injection Aβ 1-42 oligomers. Learning memory function were evaluated Morris water maze (MWM) test. In addition, hematoxylin eosin (H&E) staining, transmission electron microscopy (TEM), immunohistochemistry, ELISA western blot analysis performed evaluate...
Background Severe hindlimb ischemia is a chronic disease with poor prognosis that can lead to amputation or even death. This study aimed assess the therapeutic effect of liraglutide on hind-limb in type 2 diabetic mice and elucidate underlying mechanism. Methods Results Blood flow reperfusion capillary densities after treatment vehicle were evaluated mouse model lower-limb normal background streptozotocin-induced diabetes. The proliferation, migration, tube formation human umbilical vein...
Abstract Glycation of extracellular matrix proteins has been demonstrated to contribute the pathogenesis vascular complications. However, no previous report shown role glycated fibronectin (FN) in endothelial growth factor (VEGF)‐induced angiogenesis. Thus, this study aimed investigate effects FN on VEGF signalling and clarify molecular mechanisms involved. was incubated with methylglyoxal (MGO) vitro synthesize FN, human umbilical vein cells (HUVECs) were seeded onto unmodified MGO‐glycated...
Vascular endothelial growth factor (VEGF) plays a key role in regulating angiogenesis, and this process is largely dependent on the newly formed extracellular matrix (ECM). The levels of vitronectin (VN) are increased patients with various cardiovascular diseases. A for VN VEGF-induced angiogenesis has not been previously reported. We tested hypothesis that regulates VEGFR-2 activation via effects αvβ3, thus contributing to angiogenesis.We used 3-dimensional assay, examined VEGF-mediated...
The impairment of the angiopoietin-1 (Ang-1)/Tie-2 signaling pathway has been thought to play a critical role in diabetic complications. However, underlying mechanisms remain unclear. present study aims investigate effects Tie-2 glycation on Ang-1 activation and Ang-1-induced angiogenesis. We identified that was modified by advanced end products (AGEs) aortae derived from high fat diet (HFD)-fed mice methylglyoxal (MGO)-treated human umbilical vein endothelial cells (HUVECs). MGO-induced...
Mitsugumin 53 (MG53), which is expressed predominantly in striated muscle, has been demonstrated to be a myokine/cardiokine secreted from muscle under specific conditions. The important roles of MG53 non-striated tissues have also examined multiple disease models. However, no previous study implicated the control endothelial cell function. In order explore effects on cells, human umbilical vein cells (HUVECs) were stimulated with recombinant (rhMG53). Then, rhMG53 uptake, focal adhesion...
OBJECTIVE: Type II diabetes mellitus (DM) leads to the development of microvascular diseases and is associated with impaired angiogenesis. Elevated plasma levels plasminogen activator inhibitor-1 (PAI-1) are DM. However, role PAI-1 in DM-associated disease poorly defined. RESEARCH DESIGN AND METHODS: Hind-limb ischemia was generated by femoral artery occlusion diet-induced diabetic mice age-matched normal chow (NC)-fed mice. Plasma analyzed for glucose, non-esterified fatty acids, PAI-1....
Objective: Platelets can regulate endothelial cell genes expression through their adhesion to the subendothelium in response vascular injury. Recently it has been reported that some cells (ECs)-secreted modulate smooth muscle (VSMCs) phenotypic transformation by ECs/VSMCs co-culture. However, little is known about effects of platelets ECs interaction on VSMCs phenotype. In this study, we investigated role secreted EC regulating SMC transformation. Methods and Results: By Q-RT-PCR, PAI-1,...
Abstract Thromboembolism is a major source of morbidity and mortality in patients with cancer. Increased incidence venous thromboembolism (VTE) was associated anti-vascular endothelial growth factor (VEGF) treatment However, the mechanism VTE initiation remains elusive. In this study, we addressed effect bevacizumab, humanized monoclonal antibody against VEGF-A on either an inferior vena cava stenosis or saphenous vein model using xenograft mouse model. We found that bevacizumab accelerated...