A5100342082- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Inflammatory Bowel Disease
- Antibiotic Resistance in Bacteria
- Antifungal resistance and susceptibility
- Microbial infections and disease research
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Microscopic Colitis
- Inflammation biomarkers and pathways
- Peptidase Inhibition and Analysis
- Antimicrobial Resistance in Staphylococcus
- Cytomegalovirus and herpesvirus research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fungal Infections and Studies
- Aquaculture disease management and microbiota
- Trace Elements in Health
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- vaccines and immunoinformatics approaches
Huazhong Agricultural University
2021-2025
Center of Hubei Cooperative Innovation for Emissions Trading System
2021-2025
Royal Netherlands Academy of Arts and Sciences
2022-2025
Oncode Institute
2022-2025
Princess Máxima Center
2022-2025
Minjiang University
2023-2025
Shanghai Zhangjiang Laboratory
2024
University of California, Los Angeles
2011-2024
The People's Hospital Tongling
2024
University Medical Center Utrecht
2024
We sought to define protective mechanisms of immunity Staphylococcus aureus and Candida albicans bloodstream infections in mice immunized with the recombinant N-terminus Als3p (rAls3p-N) vaccine plus aluminum hydroxide (Al(OH3) adjuvant, or adjuvant controls. Deficiency IFN-γ but not IL-17A enhanced susceptibility control both infections. However, vaccine-induced against required CD4+ T-cell-derived IL-17A, functional phagocytic effectors. Vaccination primed Th1, Th17, Th1/17 lymphocytes,...
Opposing roles have been proposed for IL-22 in intestinal pathophysiology. We optimized human small organoid (hSIO) culturing, constitutively generating all differentiated cell types while maintaining an active stem compartment. does not promote the expansion of cells but rather slows growth hSIOs. In hSIOs, is required formation Paneth cells, prime producers antimicrobial peptides (AMPs). Introduction inflammatory bowel disease (IBD)-associated loss-of-function mutations co-receptor gene...
Extreme-drug-resistant (XDR) Acinetobacter baumannii is a rapidly emerging pathogen causing infections with unacceptably high mortality rates due to inadequate available treatment. New methods prevent and treat such are critical unmet medical need. To conduct rational vaccine discovery program, OmpA was identified as the primary target of humoral immune response after intravenous infection by A. in mice. >99% conserved at amino acid level across clinical isolates harvested between 1951 2009...
Rhizopus oryzae is the most common cause of mucormycosis, an angioinvasive fungal infection that causes more then 50% mortality rate despite first-line therapy. Clinical and animal model data clearly demonstrate presence elevated available serum iron predisposes host to mucormycosis. The high affinity permease gene (FTR1) required for R. transport in iron-depleted environments. Here we FTR1 full virulence mice. We show expressed during diabetic ketoacidosis (DKA) In addition, disrupted by...
ABSTRACT Vaccination with the recombinant N terminus of candidal adhesin Als3p (rAls3p-N) protects mice from lethal candidemia. Candidal also is structurally similar to microbial surface components recognizing adhesive matrix molecule adhesin, clumping factor, Staphylococcus aureus. To determine potential for cross-kingdom vaccination, we immunized rAls3p-N or negative control proteins and challenged them via tail vein S. aureus other gram-positive gram-negative pathogens. The vaccine, but...
ABSTRACT New treatments are needed for extensively drug-resistant (XDR) Gram-negative bacilli (GNB), such as Acinetobacter baumannii . Toll-like receptor 4 (TLR4) was previously reported to enhance bacterial clearance of GNB, including A. However, here we have shown that 100% wild-type mice versus 0% TLR4-deficient died septic shock due infection, despite having similar tissue burdens. The strain lipopolysaccharide (LPS) content and TLR4 activation by extracted LPS did not correlate with in...
TGF-β modulates immune response by suppressing non-regulatory T (Treg) function and promoting Treg function. The question of whether achieves distinct effects on non-Treg cells through discrete signaling pathways remains outstanding. In this study, we investigated the requirements Smad-dependent -independent for T-cell Smad2 Smad3 double deficiency in led to lethal inflammatory disorder mice. Non-Treg were spontaneously activated produced effector cytokines vivo deletion both Smad3....
Granzyme B (GrzB) is a serine proteinase expressed by memory T cells and NK cells. Methods to measure GrzB protein usually involve intracellular (flow cytometry) extracellular (ELISA ELISpot) assays. CD8 are the main source of during immunological reactions, but activated CD4 deploy as well. Because an important mediator cell death, tissue pathology disease, clarification differences expression secretion between for understanding effector functions these cells.Memory were purified from human...
The use of high-dose per fraction radiotherapy delivered as stereotactic body is a standard care for prostate cancer. It hypothesized that may enhance or suppress tumor-reactive immunity. objective this study was to assess both antitumor and immunosuppressive effects induced by in cancer coclinical models, ultimately, test whether combination with targeted immunotherapy can immunity.We studied the without anti-Gr-1 using syngeneic murine allograft models. dynamic change immune populations,...
Dialysis patients with chronic renal failure receiving deferoxamine for treating iron overload are uniquely predisposed mucormycosis, which is most often caused by Rhizopus oryzae. Although the siderophore not secreted Mucorales, previous studies established that species utilize from ferrioxamine (iron-rich form of deferoxamine). Here we determined CBS domain proteins Fob1 and Fob2 act as receptors on cell surface R. oryzae during uptake ferrioxamine. expression was induced in presence bound...
New strategies to treat antibiotic-resistant infections are urgently needed. We serendipitously discovered that stem cell conditioned media possessed broad antimicrobial properties. Biochemical, functional, and genetic assays confirmed the effect was mediated by supra-physiological concentrations of transferrin. Human transferrin inhibited growth gram-positive (Staphylococcus aureus), gram-negative (Acinetobacter baumannii), fungal (Candida albicans) pathogens sequestering iron disrupting...
Different pathogens share similar medical settings and rely on virulence strategies to cause infections. We have previously applied 3-D computational modeling bioinformatics discover novel antigens that target more than one human pathogen. Active passive immunization with the recombinant N-terminus of Candida albicans Hyr1 (rHyr1p-N) protect mice against lethal candidemia. Here we determine Hyr1p shares homology cell surface proteins multidrug resistant Gram negative bacterium, Acinetobacter...
Intestinal dysbiosis is thought to confer susceptibility inflammatory bowel disease (IBD), but it unknown whether dynamic changes in the microbiome contribute fluctuations activity. We explored this question using mice with intestine-specific deletion of C1galt1 (also known as T-synthase) (Tsyn mice). These develop spontaneous microbiota-dependent colitis a remitting/relapsing course due loss mucin core-1 derived O-glycans. 16S rRNA sequencing and untargeted metabolomics demonstrated...
Abstract Although TLR signaling in B cells has been implicated the germinal center (GC) responses during viral infections and autoimmune diseases, underlying mechanism is unclear. Bacterial phage Qβ-derived virus-like particle (Qβ-VLP) contains ligands, which can enhance Qβ-VLP-induced Ab response, including GC through TLR/MyD88 cells. In this study, by examining Ag-specific cell response to Qβ-VLP, we found that lack of MyD88 from beginning immune led a more severe defect scale than...
ABSTRACT Interaction between viruses and bacteria during the development of infectious diseases is a complex question that requires continuous study. In this study, we explored interactions pseudorabies virus (PRV) Pasteurella multocida (PM), which are recognized as primary secondary agents porcine respiratory disease (PRDC), respectively. vivo tests using mouse models demonstrated intranasal inoculation with PRV at sublethal dose induced disruption murine barrier promoted invasion damages...
Both protein disulfide isomerase (PDI) and SARS-CoV-2 main protease (Mpro) are reliant on active site cysteines stabilized by adjacent amino acids. We reasoned that redox compounds might interfere with both enzymes acting in the vicinity of these reactive sites thus interfering viral replication thrombus formation. Our previous screen 1019 flavonoids identified several inhibit Mpro. goal was to identify phytochemical inhibitors Mpro block thiol isomerases antithrombotic. PDI, ERp57, ERp5,...
Pasteurella multocida toxin (PMT) is an exotoxin produced by several members of the zoonotic respiratory pathogen P. multocida. The role PMT in disrupting mammalian barrier remains to be elucidated. In this study, we showed that inoculation recombinantly expressed increased permeability epithelial mouse and cell models. This was evidenced a decreased expression tight junctions (ZO-1, occludin) adherens (β-catenin, E-cadherin), as well enhanced cytoskeletal rearrangement. mechanism,...
BEST4/CA7+ cells of the human intestine were recently identified by single-cell RNA sequencing. While their gene expression profile predicts a role in electrolyte balance, cell function has not been explored experimentally owing to absence mice and paucity vitro models. Here, we establish protocol that allows emergence intestinal organoids. Differentiation requires activation Notch signaling transcription factor SPIB. numbers strongly increase response cytokine interferon-γ, supporting...
We previously reported that vaccination with Freund's adjuvant plus the recombinant N-terminus of candidal adhesin, Als3p (rAls3p-N), protects mice from disseminated candidiasis. Here we report rAls3p-N vaccine is effective when combined aluminum hydroxide adjuvant. Antibody titers > or =1:6400 accurately predicted protection infection. Nevertheless, neither B lymphocytes nor serum immunized animals transferred to vaccine-naive animals. In contrast, CD3(+), CD4(+), CD8(+) T protection, and...
Abstract Natural pathogens, such as viruses, often induce T-dependent and T-independent Ab responses. However, the activation differentiation of Ag-specific B cells under these conditions had not been examined in detail. In this study, we used bacterial phage Qβ-derived virus-like particles (Qβ-VLPs) an immunogen to examine phases response mice. Using Qβ-specific cell labeling enrichment methods developed were able characterize rare Surprisingly, found that Qβ-VLPs could Bcl-6 expression...