A5003179197- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Probiotics and Fermented Foods
- Polyamine Metabolism and Applications
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Polysaccharides Composition and Applications
- Enzyme Catalysis and Immobilization
- Mycotoxins in Agriculture and Food
- Food composition and properties
- Phytase and its Applications
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Plant Pathogenic Bacteria Studies
- Enzyme Structure and Function
- Proteins in Food Systems
- Fungal Infections and Studies
- CRISPR and Genetic Engineering
National Center for Agricultural Utilization Research
2015-2024
United States Department of Agriculture
2011-2024
Agricultural Research Service
2015-2024
Oak Ridge Associated Universities
2022
Peoria campus of the University of Illinois System
2019
Tel Aviv University
2013
Agricultural Research Organization
2013
Hebrew University of Jerusalem
2013
Seton Hall University
2012
Pace University
2012
Rhizopus oryzae is the primary cause of mucormycosis, an emerging, life-threatening infection characterized by rapid angioinvasive growth with overall mortality rate that exceeds 50%. As a representative paraphyletic basal group fungal kingdom called "zygomycetes," R. also used as model to study evolution. Here we report genome sequence strain 99-880, isolated from fatal case mucormycosis. The highly repetitive 45.3 Mb assembly contains abundant transposable elements (TEs), comprising...
Mucormycosis causes mortality in at least 50% of cases despite current first-line therapies. Clinical and animal data indicate that the presence elevated available serum iron predisposes host to mucormycosis. Here we demonstrate deferasirox, an chelator recently approved for use humans by US FDA, is a highly effective treatment Deferasirox effectively chelated from Rhizopus oryzae demonstrated cidal activity vitro against 28 29 clinical isolates Mucorales concentrations well below clinically...
DNA isolated from the wild-type aflatoxin-producing (Afl+) fungus Aspergillus parasiticus NRRL 5862 was used to construct a cosmid genomic library employing homologous gene (pyrG) encoding orotidine monophosphate decarboxylase for selection of fungal transformants. The transformed into an Afl- mutant, A. CS10 (ver-1 wh-1 pyrG), deficient in conversion aflatoxin biosynthetic intermediate versicolorin A sterigmatocystin. One pyrG+ Afl+ transformant identified. fragments this transformant,...
Rhizopus oryzae is the most common cause of mucormycosis, an angioinvasive fungal infection that causes more then 50% mortality rate despite first-line therapy. Clinical and animal model data clearly demonstrate presence elevated available serum iron predisposes host to mucormycosis. The high affinity permease gene (FTR1) required for R. transport in iron-depleted environments. Here we FTR1 full virulence mice. We show expressed during diabetic ketoacidosis (DKA) In addition, disrupted by...
Abstract Mucormycosis is a life-threatening infection caused by Mucorales fungi. Here we sequence 30 fungal genomes, and perform transcriptomics with three representative Rhizopus Mucor strains human airway epithelial cells during invasion, to reveal key host determinants contributing pathogenesis. Analysis of the transcriptional response reveals platelet-derived growth factor receptor B (PDGFRB) signaling as part core divergent pathogenic fungi; inhibition PDGFRB reduces Mucorales-induced...
Environmental isolates of the fungus Rhizopus have been shown to harbor a bacterial endosymbiont (Burkholderia) that produces rhixozin, plant mycotoxin. We sought define role rhizoxin production by endosymbionts in pathogenesis mucormycosis. Endosymbiotic bacteria were identified polymerase chain reaction 15 (54%) 28 clinical Zygomycetes, with 33% strains showing 87% identity Burkholderia 16S rDNA. The presence myclial extracts from fungi harboring was confirmed high-performance liquid...
Dialysis patients with chronic renal failure receiving deferoxamine for treating iron overload are uniquely predisposed mucormycosis, which is most often caused by Rhizopus oryzae. Although the siderophore not secreted Mucorales, previous studies established that species utilize from ferrioxamine (iron-rich form of deferoxamine). Here we determined CBS domain proteins Fob1 and Fob2 act as receptors on cell surface R. oryzae during uptake ferrioxamine. expression was induced in presence bound...
ABSTRACT Rhizopus oryzae is used for industrial production of lactic acid, yet little known about the genetics this fungus. In study I cloned two genes, ldhA and ldhB , which code NAD + -dependent l -lactate dehydrogenases (LDH) (EC 1.1.1.27 ), from a acid-producing strain R. . These genes are similar to each other exhibit more than 90% nucleotide sequence identity they contain no introns. This first description ldh in fungus, comparisons revealed that these distinct previously isolated...
Plant cell walls have been shown to contain acetyl groups in hemicelluloses and pectin. The gene aes1, encoding the esterase (Aes1) of Hypocrea jecorina, was identified by amino-terminal sequencing, peptide mass spectrometry, genomic sequence analyses. coded polypeptide had 348 amino acid residues with first 19 serving as a secretion signal peptide. calculated molecular isoelectric point secreted enzyme were 37,088 Da pH 5.89, respectively. No significant homology found between predicated...
RNA transcript accumulation for the ver-1 and nor-1 genes, which are associated with aflatoxin biosynthesis in fungus Aspergillus parasiticus, was measured before during production liquid shake culture. Transcripts were not detected until near end of trophophase (growth phase) could still be observed well into stationary phase batch fermentation an aflatoxin-supporting growth medium. Maximum both transcripts occurred just prior to onset phase. Aflatoxin B1 first approximately 8 h after...
ABSTRACT Efficient and rapid production of value‐added chemicals from lignocellulosic biomass is an important step toward a sustainable society. Lactic acid, used for synthesizing the bioplastic polylactide, has been produced by microbial fermentation using primarily glucose. Lignocellulosic hydrolysates contain high concentrations cellobiose xylose. Here, we constructed recombinant Saccharomyces cerevisiae strain capable fermenting xylose into lactic acid. Specifically, genes ( cdt‐1 ,...
Rhizopus delemar and associated species attack a wide range of fruit vegetables after harvest. Host nutrients acidic pH are required for optimal germination R. delemar, we studied how this process is triggered. Glucose induced spore swelling in an environment, expressed by up to 3-fold increase diameter, whereas diameter was smaller neutral environment. When suspended the spores started float, indicating change their density. Treatment with HgCl2, aquaporin blocker, prevented floating...
Glucansucrases, which can be produced by different Lactic Acid Bacteria (LAB), catalyze the synthesis of α-glucans with structures and properties using sucrose as substrate. In this study, a novel glucansucrase (GTFA) from Lactobacillus reuteri E81 was identified heterologously expressed. Alignments GTFA other glucansucrases revealed its novelty putative 3D model structure obtained. The biochemical truncated enzyme without N-terminal variable region, GTFA-ΔN, characterized. Km Vmax were...