A5022751421- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Photosynthetic Processes and Mechanisms
- Catalysis for Biomass Conversion
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Pancreatic function and diabetes
- Micro and Nano Robotics
- Advanced Thermodynamics and Statistical Mechanics
- Plant responses to water stress
- RNA and protein synthesis mechanisms
- Enzyme Catalysis and Immobilization
- Algal biology and biofuel production
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Diet, Metabolism, and Disease
- Protist diversity and phylogeny
- Polyamine Metabolism and Applications
University of Illinois Urbana-Champaign
2014-2021
Tianjin University
2011
Industrial polyploid yeast strains harbor numerous beneficial traits but suffer from a lack of available auxotrophic markers for genetic manipulation. Here we demonstrated quick and efficient strategy to generate in industrial with the RNA-guided Cas9 nuclease. We successfully constructed quadruple mutant popular strain, Saccharomyces cerevisiae ATCC 4124, ura3, trp1, leu2, his3 auxotrophies through Even though multiple alleles marker genes had be disrupted simultaneously, observed knockouts...
Saccharomyces boulardiiis a probiotic yeast that has been used for promoting gut health as well preventing diarrheal diseases. This not only exhibits beneficial phenotypes but also can stay longer in the than cerevisiae Therefore, S. an attractive host metabolic engineering to produce biomolecules of interest gut. However, lack auxotrophic strains with defined genetic backgrounds hampered use this strain engineering. Here, we report development well-defined mutants (leu2,ura3,his3, and trp1)...
Microorganisms commonly exhibit preferential glucose consumption and diauxic growth when cultured in mixtures of other sugars. Although various genetic perturbations have alleviated the effects repression on specific sugars, a broadly applicable mechanism remains unknown. Here, we report that reduction rate phosphorylation alleviates Saccharomyces cerevisiae. Through adaptive evolution under mixture xylose analog 2-deoxyglucose, isolated mutant strain capable simultaneously consuming xylose....
Abstract Isomerases perform biotransformations without cofactors but often cause an undesirable mixture of substrate and product due to unfavorable thermodynamic equilibria. We demonstrate the feasibility using engineered yeast strain harboring oxidoreductase reactions overcome limit isomerization reaction. Specifically, a capable consuming lactose intracellularly is produce tagatose from through three layers manipulations. First, GAL1 coding for galactose kinase deleted eliminate...
Global climate change caused by the emission of anthropogenic greenhouse gases (GHGs) is a grand challenge to humanity. To alleviate trend, consumption fossil fuels needs be largely reduced and alternative energy technologies capable controlling GHG emissions are anticipated. In this study, we introduced synthetic reductive pentose phosphate pathway (rPPP) into xylose-fermenting Saccharomyces cerevisiae strain SR8 achieve simultaneous lignocellulosic bioethanol production carbon dioxide...
ABSTRACT The probiotic yeast Saccharomyces boulardii has been extensively studied for the prevention and treatment of diarrheal diseases, it is now commercially available in some countries. S. displays notable phenotypic characteristics, such as a high optimal growth temperature, tolerance against acidic conditions, inability to form ascospores, which differentiate from cerevisiae . majority prior studies stated that exhibits sluggish or halted galactose utilization. Nonetheless, molecular...
Microorganisms have evolved to produce specific end products for many reasons, including maintaining redox balance between NAD+ and NADH. The yeast Saccharomyces cerevisiae, example, produces ethanol as a primary product from glucose the regeneration of NAD+. Engineered S. cerevisiae strains been developed ferment lignocellulosic sugars, such xylose, lactic acid by expression heterologous lactate dehydrogenase (ldhA Rhizopus oryzae) without genetic perturbation native pathway. Surprisingly,...