A5078146033- Fungal and yeast genetics research
- Enzyme-mediated dye degradation
- Fungal Biology and Applications
- Algal biology and biofuel production
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Heat shock proteins research
- bioluminescence and chemiluminescence research
- Protein Structure and Dynamics
- Chromium effects and bioremediation
- Antifungal resistance and susceptibility
- Protein Interaction Studies and Fluorescence Analysis
- Endoplasmic Reticulum Stress and Disease
- Metal-Catalyzed Oxygenation Mechanisms
- Amino Acid Enzymes and Metabolism
- Mycotoxins in Agriculture and Food
- Microbial Metabolic Engineering and Bioproduction
- Redox biology and oxidative stress
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial biofilms and quorum sensing
- Arsenic contamination and mitigation
- Gene Regulatory Network Analysis
- Marine Toxins and Detection Methods
- Biocrusts and Microbial Ecology
Universidad Nacional Autónoma de México
2008-2023
Instituto de Química y Fisicoquímica Biológicas
2006
Instituto Nacional de Cardiología
2002-2005
Heinrich Heine University Düsseldorf
1993
Universidad Autónoma del Estado de Morelos
1986
Rhizopus oryzae is the primary cause of mucormycosis, an emerging, life-threatening infection characterized by rapid angioinvasive growth with overall mortality rate that exceeds 50%. As a representative paraphyletic basal group fungal kingdom called "zygomycetes," R. also used as model to study evolution. Here we report genome sequence strain 99-880, isolated from fatal case mucormycosis. The highly repetitive 45.3 Mb assembly contains abundant transposable elements (TEs), comprising...
We have proposed that reactive oxygen species (ROS) play essential roles in cell differentiation. Enzymes belonging to the NADPH oxidase (NOX) family produce superoxide a regulated manner. identified three distinct NOX subfamilies fungal kingdom and shown NoxA is required for sexual differentiation Aspergillus nidulans. Here we show Neurospora crassa NOX-1 elimination results complete female sterility, decreased asexual development, reduction of hyphal growth. The lack NOX-2 did not affect...
Different bands of catalase activity in zymograms (Cat-1a-Cat-1e) appear during Neurospora crassa development and under stress conditions. Here we demonstrate that singlet oxygen modifies Cat-1a, giving rise to a sequential shift electrophoretic mobility, similar the one observed vivo. Purified Cat-1a was modified with generated from photosensitization reaction; even when reaction separated enzyme by an air barrier, condition which only can reach diffusion. Modification hindered reducing...
Reactive oxygen species (ROS) such as hydrogen peroxide, produced externally or during normal metabolism, can damage different cell components and usually trigger a counteracting antioxidant response. The fact that animals humans utilize ROS related nitrogen reactive to prevent fungal infection has generated great interest in defining the of response studying their role virulence determinants fungi. Here we review specific enzyme non-enzyme mediated mechanisms virulence, well signal...
ABSTRACT We use asexual development of Neurospora crassa as a model system with which to determine the causes cell differentiation. Air exposure mycelial mat induces hyphal adhesion, and adherent hyphae grow aerial that, in turn, form conidia. Previous work indicated hyperoxidant state at start these morphogenetic transitions large increase catalase activity during conidiation. Catalase 3 (CAT-3) increases end exponential growth is induced by different stress conditions. Here we analyzed...
Neurospora crassa glutamine synthetase mRNA was measured by its capacity to direct the synthesis of specific protein in a cell-free system derived from rabbit reticulocytes. N. cultures grown on glutamate as sole nitrogen source had higher activities than did those glutamine. The differences were about 10-fold when polysomal RNA used for translation and 5-fold either total cellular or polyadenylic acid-enriched used. These data indicate that exponentially growing crassa, regulates adjusting levels.
The conidiation process of Neurospora crassa is characterized by three morphogenetic steps: hyphal adhesion, aerial formation, and production conidia. Previous data indicated the occurrence a hyperoxidant state at onset all steps. Because glutamine synthetase (GS) biosynthetic glutamate dehydrogenase [GDH(NADP)] enzymes are susceptible to inactivation reactive oxygen species, we followed these enzyme activities during under different physiological conditions related them states...