A5055415104- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fungal and yeast genetics research
- Genomics, phytochemicals, and oxidative stress
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Nuclear Receptors and Signaling
- MicroRNA in disease regulation
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Protist diversity and phylogeny
- Plant Gene Expression Analysis
- Algal biology and biofuel production
- Heat shock proteins research
- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- Enzyme-mediated dye degradation
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Microbial metabolism and enzyme function
Universidad Nacional Autónoma de México
2014-2024
Center for Scientific Research and Higher Education at Ensenada
2023
We have proposed that reactive oxygen species (ROS) play essential roles in cell differentiation. Enzymes belonging to the NADPH oxidase (NOX) family produce superoxide a regulated manner. identified three distinct NOX subfamilies fungal kingdom and shown NoxA is required for sexual differentiation Aspergillus nidulans. Here we show Neurospora crassa NOX-1 elimination results complete female sterility, decreased asexual development, reduction of hyphal growth. The lack NOX-2 did not affect...
NADPH oxidase (NOX)-derived reactive oxygen species (ROS) act as signaling determinants that induce different cellular processes. To characterize NOX function during fungal development, we utilized the genetically tractable ascomycete Sordaria macrospora. Genome sequencing of a sterile mutant led us to identify encoding nox1 gene required for fruiting body formation, regular hyphal growth, and fusion. These phenotypes are shared by nor1, lacking regulator NOR1. Further phenotypic analyses...
The regulation of gene expression in response to increased levels reactive oxygen species (ROS) is a ubiquitous aerobic organisms. However, different organisms use strategies perceive and respond high ROS levels. Yeast Yap1 paradigmatic example specific mechanism used by eukaryotic cells link sensing regulation. activation this transcription factor H2O2 mediated peroxiredoxins, which are widespread enzymes that cysteine thiols sense ROS, as well catalyze the reduction peroxides water. In...
The NADPH oxidases (NOX) catalyze the production of superoxide by transferring electrons from to O2, in a regulated manner. In Neurospora crassa NOX-1 is required for normal growth hyphae, development aerial mycelium and asexual spores, it essential sexual differentiation cell-cell fusion. Determining subcellular localization critical step understanding mechanisms which this enzyme can regulate all these different processes. Using fully functional versions tagged with mCherry, we show that...
Massive testing is a cornerstone in efforts to effectively track infections and stop COVID-19 transmission, including places with good vaccination coverage. However, SARS-CoV-2 by RT-qPCR requires specialized personnel, protection equipment, commercial kits, dedicated facilities, which represent significant challenges for massive resource-limited settings. It therefore important develop protocols that are inexpensive, fast, sufficiently sensitive. Here, we optimized the composition of buffer...
Diabetes complications such as nephropathy, retinopathy, or cardiovascular disease arise from vascular dysfunction. In this context, it has been observed that past hyperglycemic events can induce long-lasting alterations, a phenomenon termed "metabolic memory." study, we evaluated the genome-wide gene expression and chromatin accessibility alterations caused by transient high-glucose exposure in human endothelial cells (ECs) vitro. We found exposed to high glucose exhibited substantial...
ABSTRACT Various diabetes complications, including nephropathy, retinopathy, and cardiovascular disease, arise from vascular dysfunction. In this context, it has been observed that past hyperglycaemic events can induce long-lasting transcriptional changes, a phenomenon termed “metabolic memory”. Yet, the underlying mechanisms driving these persistent effects are not fully characterized. study, we evaluated genome-wide gene expression chromatin accessibility alterations caused by transient...
ABSTRACT Massive testing is a cornerstone in efforts to effectively track infections and stop COVID-19 transmission, including places where good vaccination coverage has been achieved. However, SARS-CoV-2 by RT-qPCR requires specialized personnel, protection equipment, commercial kits, dedicated facilities, which represent significant challenges for massive implementation resource-limited settings. It therefore important develop protocols that facilitate are inexpensive, fast, sufficiently...