A5069470970- Erythrocyte Function and Pathophysiology
- Enzyme Structure and Function
- Tryptophan and brain disorders
- Reproductive tract infections research
- Stress Responses and Cortisol
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Neonatal Health and Biochemistry
- Research on Leishmaniasis Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Protein Structure and Dynamics
- Reproductive System and Pregnancy
- Ubiquitin and proteasome pathways
- Enzyme-mediated dye degradation
- Circadian rhythm and melatonin
- Cancer Research and Treatments
- Material Science and Thermodynamics
- Pancreatic function and diabetes
- HIV/AIDS drug development and treatment
- Chromium effects and bioremediation
- Synthesis and Biological Activity
- Urologic and reproductive health conditions
- Nanomaterials for catalytic reactions
- Diet, Metabolism, and Disease
- Marine Sponges and Natural Products
Instituto Nacional de Psiquiatría
2015-2025
Universidad Nacional Autónoma de México
2003-2011
Center for Research and Advanced Studies of the National Polytechnic Institute
2008-2010
Human triosephosphate isomerase deficiency is a rare autosomal disease that causes premature death of homozygous individuals. The most frequent mutation leads to this illness in position 104, which involves conservative change Glu for Asp. Despite the extensive work has been carried out on E104D mutant enzyme hemolysates and whole cells, molecular basis poorly understood. Here, we show purified, recombinant E104D, while exhibiting normal catalytic activity, shows impairments formation active...
Changes in cytokine levels major depression and during treatment have been reported adults. However, few studies examined an adolescent sample despite this being a common age of onset. Methods . We measured proinflammatory (IL-2, IFN- γ , IL-1 β TNF- α IL-6, IL-12, IL-15) anti-inflammatory (IL-4, IL-5, IL-13, IL-1Ra, IL-10) serum 22 adolescents with 18 healthy volunteers. Cytokines were by multiplex bead-based immunoassays at baseline, 4 8 weeks after commencement fluoxetine administration...
Major depressive disorder (MDD) has a prevalence of 5% in adolescents. Several studies have described the association between inflammatory response and MDD, but little is known about relationship MDD growth factors, such as IL-7, IL-9, IL-17A, VEGF, basic FGF, G-CSF, GM-CSF. It must be appointed that there are scarce reports on factors adolescents with even fewer clinical follow-up. In this work, we evaluated levels (IL-7, GM-CSF) follow-up during eight weeks treatment fluoxetine. Methods ....
ABSTRACT Metacaspases (MCA), are cysteine‐dependent proteases closely related to caspases. In protozoa, MCA plays an important role in programmed cell death (PCD). Trichomonas vaginalis , a kind of PCD that resembles apoptosis has been described, but the activators this mechanism have not demonstrated. We performed genome‐wide silico analysis T. database using consensus domains. A total 15 protein annotations for MCA‐like sequences were retrieved. Only 7/15 (TvMCA1‐6 and TvMCA9) annotated as...
Abstract Glutathione S ‐transferases (GSTs) are a family of detoxifying enzymes that catalyze the conjugation glutathione (GSH) to electrophiles, thereby increasing solubility xenobiotics and aiding its excretion from cell. The present work presents inhibition mu‐class GST marine shrimp Litopenaeus vannamei by copper (Cu 2+ ) cadmium (Cd ). protein was overexpressed in bacteria enzymatic activity measured using 1‐chloro‐2,4‐dinitrobenzene. mean inhibitory concentration (IC 50 for against Cu...
Monomeric Ubiquitin (Ub) is a 76-amino acid highly conserved protein found in eukaryotes. The first described biological activity of Ub the 1970s was extracellular, but it quickly gained relevance due to its intracellular role, i.e., post-translational modification proteins (ubiquitination) that regulate numerous eukaryotic cellular processes. In following years, extracellular role relegated background, until correlation between higher survival rate and increased serum concentrations...
Glucosamine‐6‐phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D ‐glucosamine‐6‐phosphate into ‐fructose‐6‐phosphate and ammonium. Here we describe existence two mammalian glucosamine‐6‐phosphate enzymes. We present crystallographic structure one them, long human deaminase, at 1.75 Å resolution. Crystals belong to space group P2 1 2 a whole hexamer in asymmetric unit. The active‐site lid (residues 162–182) presented significant structural...
Murine leprosy, caused by Mycobacterium lepraemurium (MLM), is a chronic disease that closely resembles human leprosy. Even though this does not directly involve the nervous system, we investigated possible effect on working memory during infection in Balb/c mice. We evaluated alterations dorsal region of hippocampus and measured peripheral levels cytokines at 40, 80, 120 days post-infection. To evaluate memory, used T-maze while morphometric analysis was conducted regions CA1, CA2, CA3,...
Monomeric ubiquitin (Ub) is a 76-amino-acid highly conserved protein found in eukaryotes. The biological activity of Ub first described the 1970s was extracellular, but it quickly gained relevance due to its intracellular role, i.e., post-translational modification proteins (ubiquitination) that regulate numerous eukaryotic cellular processes. In following years, extracellular role relegated background, until correlation between higher survival rate and increased serum concentrations...
To understand whether protein Tv-PSP1 from Trichomonas vaginalis recognizes mRNA parasite stem-loop structures, we conducted REMSA and intrinsic fluorescence assays. We found the recombinant structure, determined with X-ray crystallography, showed unusual thermal stability of quaternary associated a disulfide bridge CYS76-CYS104. gain deeper insight into interaction stem-loops (mRNAsl) its relationship stability, also used an integrated computational protocol that combined molecular dynamics...