A5046817854- Aquaculture disease management and microbiota
- Protein Hydrolysis and Bioactive Peptides
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Biochemical and Molecular Research
- Allergic Rhinitis and Sensitization
- Glutathione Transferases and Polymorphisms
- Aquaculture Nutrition and Growth
- Food Allergy and Anaphylaxis Research
- Asthma and respiratory diseases
- Meat and Animal Product Quality
- Vibrio bacteria research studies
- vaccines and immunoinformatics approaches
- Antimicrobial Peptides and Activities
- Porphyrin and Phthalocyanine Chemistry
- Phytochemistry and Bioactive Compounds
- Genomics, phytochemicals, and oxidative stress
- Environmental Toxicology and Ecotoxicology
- SARS-CoV-2 and COVID-19 Research
- Insect Resistance and Genetics
- Plant Gene Expression Analysis
- Phytochemical compounds biological activities
- Tuberculosis Research and Epidemiology
- Alcoholism and Thiamine Deficiency
- Insect Utilization and Effects
Universidad de Sonora
2015-2025
Universidad de Hermosillo
2021
Universidad Estatal de Sonora
2020
Centro de Investigación en Alimentación y Desarrollo
2008-2010
Chickpea (Cicer arietinum L.) peptides have shown in vitro potential to inhibit the angiotensin I-converting enzyme (ACE-I). However, molecular interactions between chickpea (CP) and ACE-I as well their ADMET (absorption/distribution/metabolism/excretion/toxicity) characteristics remain unknown. Thus, our aim was study silico of CP with characteristics. Legumin provicilin sequences were submitted analysis search for inhibitory peptides. Simulated enzymatic hydrolysis performed using...
Abstract Glutathione S ‐transferases (GSTs) are a family of detoxifying enzymes that catalyze the conjugation glutathione (GSH) to electrophiles, thereby increasing solubility xenobiotics and aiding its excretion from cell. The present work presents inhibition mu‐class GST marine shrimp Litopenaeus vannamei by copper (Cu 2+ ) cadmium (Cd ). protein was overexpressed in bacteria enzymatic activity measured using 1‐chloro‐2,4‐dinitrobenzene. mean inhibitory concentration (IC 50 for against Cu...
Vibrio parahaemolyticus is a pathogenic bacterium that threatens food safety by infecting humans and marine organisms. Among its virulence factors, lecithin-dependent hemolysin (vpLDH) has been identified as promising target for attenuating pathogenicity. This study explores the inhibitory mechanisms of three natural flavonoids—quercetin, morin, EGCG—on LDH using enzyme kinetics, fluorescence quenching, molecular dynamics simulations. The flavonoids quercetin, EGCG inhibited vpLDH...
Vibrio parahaemolyticus ( Vp ), a typical microorganism inhabiting marine ecosystems, uses pathogenic virulence molecules such as hemolysins to cause bacterial infections of both human and animals. The thermolabile hemolysin TLH lyses erythrocytes by phospholipase B/A2 enzymatic activity in egg-yolk lecithin. However, few studies have been characterized the biochemical properties use molecular target for natural compounds an alternative control infection. Here, we evaluated inhibition...
The SARS-CoV-2 virus was detected for the first time in December 2019 Wuhan, China. Currently, this has spread around world, and new variants have emerged. This pandemic provoked rapid development of diagnostic tools, therapies vaccines to control disease called COVID-19. Antibody detection by ELISA been broadly used recognize number persons infected with or evaluate response vaccinated individuals. As spread, questions arose, such as prevalence antibodies after natural infection induced...
Enzymatic activity is dependent on temperature, although some proteins have evolved to retain at low temperatures the expense of stability. Cold adapted enzymes are present in a variety organisms and there ample interest their structure-function relationships. Lysozyme (E.C. 3.2.1.17) one most studied due its antibacterial against Gram positive bacteria also cold protein. In this work characterization lysozyme from insect Manduca sexta presented. Both M. lysozymes natural recombinant showed...
Connective tissue (CT) proteins in jumbo squid (Dosidicus gigas) play an important role because they are the responsible for union between various cells; hence, there is a close relationship their functions and muscle firmness during ice storage. In this study, thermal resistance solubility of CT extracted from fins, mantle arms storage (20 days) was evaluated. The fractionated based on [NaCl-soluble (SSCT) insoluble (ICT)]. affected An increase SSCT after 10 days with subsequent decrease...
Chickpea (Cicer arietinum L.) peptides can inhibit dipeptidyl peptidase IV (DPP-IV), an important type 2 diabetes mellitus therapeutic target. The molecular interactions between the inhibitory and active site of DPP-IV have not been thoroughly examined, nor their pharmacokinetic properties. Therefore, predictions legumin- provicilin-derived peptides, with DPP-IV, properties were carried out. Ninety-two unique identified. Papain trypsin enzymes highest AE (0.0927) lowest BE (6.8625 × 10−7)...
DNA replication requires processivity factors that allow replicative polymerases to extend long stretches of DNA. Some viruses encode their own polymerase, such as the white spot syndrome virus (WSSV) infects decapod crustaceans but still require host accessory factors. We have determined by X-ray diffraction three-dimensional structure Pacific leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member sliding clamp family proteins, binds and repair...
Background and objectives: The epidemiology of food allergy (FA) food-dependent anaphylaxis remains unknown in Colombia. Our aim was to estimate by parent-report the prevalence FA a Colombian population schoolchildren. Materials methods: A printed questionnaire sent parents schoolchildren aged 5–12 years old from Medellín, Colombia order collect FA-related data. Results: Nine hundred sixty-nine (969) returned with valid responses (response rate, 52.5%). estimated rates (95% CI) were: adverse...
The SARS-CoV-2 virus was detected for the first time in December 2019 Wuhan, China. Currently, this has spread around world, and new variants have emerged. This pandemic provoked rapid development of diagnostic tools, therapies vaccines to control disease called COVID-19. Antibody detection by ELISA been broadly used recognize number persons infected with or evaluate response vaccinated individuals. As spread, questions arose, such as prevalence antibodies after natural infection induced...
Na + /K -ATPase is an essential transmembrane enzyme found in all mammalian cells with critical functions for cell ion homeostasis. The inhibition of this by several cardiotonic steroids (CTS) has been associated the cytotoxic effect on cancer lines phytochemicals such as ouabain and digitoxin. This study evaluated inhibitory capacity cardenolides calotropin corotoxigenin 3-O-glucopyranoside (C3OG) from Asclepias subulata over activity vitro silico . assays showed that C3OG decreased IC 50...
Proliferating cell nuclear antigen (PCNA) is the eukaryotic sliding clamp that tethers DNA polymerase to during replication. The full-length cDNA of Pacific white shrimp Litopenaeus vannamei PCNA (LvPCNA) was cloned and encoded a protein 260 amino acids highly similar other Crustacean PCNAs. theoretical structure has all domains are necessary for its interaction with template polymerase. RT-PCR analysis showed LvPCNA expressed mainly in muscle hemocytes much less hepatopancreas gills. mRNA...
White spot syndrome virus (WSSV) is the causative agent of white syndrome, one most devastating diseases in shrimp aquaculture. The genome WSSV includes a gene that encodes putative family B DNA polymerase (ORF514), which 16% identical amino acid sequence to Herpes 1 polymerase. aim this work was demonstrate activity ORF514-encoded protein as and hence antiviral target. A 3.5 kbp fragment encoding conserved exonuclease domains ORF514 overexpressed bacteria. recombinant showed but with very...