A5012131316- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Biochemical and Structural Characterization
- Cardiac Valve Diseases and Treatments
- Orthopedic Infections and Treatments
- Bacterial Genetics and Biotechnology
- Neonatal and Maternal Infections
- Immune Response and Inflammation
- Fungal Infections and Studies
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Microbial Metabolism and Applications
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- RNA and protein synthesis mechanisms
- Mycobacterium research and diagnosis
- Plant Pathogenic Bacteria Studies
Harbor–UCLA Medical Center
2015-2024
UCLA Medical Center
2015-2024
University of California, Los Angeles
2015-2024
The Lundquist Institute
2012-2024
Los Angeles Medical Center
2006-2024
Biomedical Research Institute
2005-2019
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2017
Olive View-UCLA Medical Center
2017
Office of Infectious Diseases
1981-2016
American Heart Association
1997-2015
Evidence-based guidelines for the management of patients with methicillin-resistant Staphylococcus aureus (MRSA) infections were prepared by an Expert Panel Infectious Diseases Society America (IDSA). The are intended use health care providers who adult and pediatric MRSA infections. discuss a variety clinical syndromes associated disease, including skin soft tissue (SSTI), bacteremia endocarditis, pneumonia, bone joint infections, central nervous system (CNS) Recommendations provided...
Background— Despite advances in medical, surgical, and critical care interventions, infective endocarditis remains a disease that is associated with considerable morbidity mortality. The continuing evolution of antimicrobial resistance among common pathogens cause creates additional therapeutic issues for physicians to manage this potentially life-threatening illness. Methods Results— This work represents the third iteration an “treatment” document developed by American Heart Association...
Evidence-based guidelines for the management of patients with methicillin-resistant Staphylococcus aureus (MRSA) infections were prepared by an Expert Panel Infectious Diseases Society America (IDSA). The are intended use health care providers who adult and pediatric MRSA infections. discuss a variety clinical syndromes associated disease, including skin soft tissue (SSTI), bacteremia endocarditis, pneumonia, bone joint infections, central nervous system (CNS) Recommendations provided...
OR DECADES, INFECTIVE ENDOCARditis (IE) caused by Staphylococcus aureus has been viewed primarily as a community-acquired disease, especially associated with injection drug use. [1]][10][11] S IE is relatively infrequent at any individual institution, and observations of its characteristics were based upon small samples, 1,3,6,9,[12][13][14] single-center experiences, 5,6,8,9,[13][14][15][16] or retrospectively identified patients. 2,7,8,15,16tient characteristics, treatment practices,...
Necrotizing fasciitis is a life-threatening infection requiring urgent surgical and medical therapy. Staphylococcus aureus has been very uncommon cause of necrotizing fasciitis, but we have recently noted an alarming number these infections caused by community-associated methicillin-resistant S. (MRSA).
DAJANI, ADNAN S.; TAUBERT, KATHRYN A.; WILSON, WALTER; BOLGER, ANN F.; BAYER, ARNOLD; FERRIERI, PATRICIA; GEWITZ, MICHAEL H.; SHULMAN, STANFORD T.; NOURI, SORAYA; NEWBUGER, JANE W.; HUTTO, CECILIA; PALIASCH, THOMASJ; GAGE, TOMMY LEVISON, MATTHEW E.; PETER, GEORGES; ZUCCARO, GREGORY JR.; Wiklund, Richard A. M.D. Author Information
Abstract The microbiology, epidemiology, diagnostics, and treatment of infective endocarditis (IE) have changed significantly since the Duke Criteria were published in 1994 modified 2000. International Society for Cardiovascular Infectious Diseases (ISCVID) convened a multidisciplinary Working Group to update diagnostic criteria IE. resulting 2023 Duke-ISCVID IE propose significant changes, including new microbiology diagnostics (enzyme immunoassay Bartonella species, polymerase chain...
Many bacterial pathogens achieve resistance to defensin-like cationic antimicrobial peptides (CAMPs) by the multiple peptide factor (MprF) protein. MprF plays a crucial role in Staphylococcus aureus virulence and it is involved CAMP-like antibiotic daptomycin. large membrane protein that modifies anionic phospholipid phosphatidylglycerol with l-lysine, thereby diminishing affinity for CAMPs. Its widespread occurrence recommends as target novel antimicrobials, although mode of action has...
ABSTRACT Increasingly frequent reports have described the in vivo loss of daptomycin susceptibility association with clinical treatment failures. The mechanism(s) resistance is not well understood. We studied an isogenic set Staphylococcus aureus isolates from bloodstream a daptomycin-treated patient recalcitrant endocarditis which serial strains exhibited decreasing to daptomycin. Since membrane-targeting lipopeptide, we compared number membrane parameters initial blood isolate (parental)...
We used daptomycin plus antistaphylococcal β-lactams (ASBL) to clear refractory MRSA bacteremia. In vitro studies showed enhanced bactericidal activity, increased membrane binding, and decrease in positive surface charge induced by ASBLs against nonsusceptible MRSA. Addition of may be benefit (Although the official designation is "daptomycin nonsusceptiblity," we will use term "daptomycin-resistance" this paper for facility presentation.)
Abstract Background The 2023 Duke–International Society of Cardiovascular Infectious Diseases (ISCVID) criteria for infective endocarditis (IE) were introduced to improve classification IE research and clinical purposes. External validation studies are required. Methods We studied consecutive patients with suspected referred the team Amsterdam University Medical Center (from October 2016 March 2021). An international expert panel independently reviewed case summaries assigned a final...
The causes of persistent bacteremia (PB) due to methicillin-resistant Staphylococcus aureus (MRSA) are poorly understood. This investigation examined potential associations between PB with key clinical features and several in vitro bacterial genotypic phenotypic characteristics, isolates from 1 institution.Pulsed-field gel electrophoresis (PFGE) relatedness, thrombin-induced platelet microbicidal protein (tPMP)-susceptibility phenotype, accessory gene regulator (agr) genotype functionality...
Microbial pathogenicity in Staphylococcus aureus is a complex process involving number of virulence genes that are regulated by global regulatory systems including sar and agr. To evaluate the roles these two loci virulence, we constructed sar-/agr- mutants strains RN6390 RN450 compared their phenotypic profiles to corresponding single sar- agr- parents. The secretion all hemolysins was absent while residual beta-hemolysin activity remained mutants. fibronectin binding capacity significantly...
Background. Staphylococcus aureus native valve infective endocarditis (SA-NVIE) is not completely understood. The objective of this investigation was to describe the characteristics a large, international cohort patients with SA-NVIE. Methods. International Collaboration on Endocarditis Merged Database (ICE-MD) combination 7 existing electronic databases from 5 countries that contains data 2212 cases definite (IE). Results. Of IE, 566 (34%) had IE due S. aureus, and 1074 pathogens other than...
The Gram-positive bacterium Staphylococcus aureus infects diverse tissues and causes a wide spectrum of diseases, suggesting that it possesses repertoire distinct molecular mechanisms promoting bacterial survival in disparate vivo environments. Signature-tag transposon mutagenesis screening 1520-member library identified numerous S. genetic loci affecting growth four complementary animal infection models including mouse abscess, bacteraemia wound rabbit endocarditis. Of total 237 attenuated...