Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared extraintestinal E. (ExPEC) isolated from clinical cases of humans extra-intestinal diseases such urinary tract infections (UTI) and bacteremia. PCR results showed genes usually found ColV plasmid (tsh, iucA, iss, hlyF)...

Microbial pathogenicity in Staphylococcus aureus is a complex process involving number of virulence genes that are regulated by global regulatory systems including sar and agr. To evaluate the roles these two loci virulence, we constructed sar-/agr- mutants strains RN6390 RN450 compared their phenotypic profiles to corresponding single sar- agr- parents. The secretion all hemolysins was absent while residual beta-hemolysin activity remained mutants. fibronectin binding capacity significantly...

Platelet aggregation by bacteria is felt to play an important role in the pathogenesis of infective endocarditis. However, mechanisms involved bacterium-induced platelet are not well-defined. In present study, we examined which Staphylococcus aureus causes rabbit vitro. normal plasma, kinetics S. aureus-induced were rapid and biphasic. The onset magnitude phase 1 varied with bacterium-platelet ratio, maximal observed at a ratio 5:1. 2 was delayed presence apyrase (an ADP hydrolase),...

This study determined the prevalence of reactive HIV serology at 12 months age in infants exposed to utero. Of 80 patients analyzed, 50 (63.3%) were anti-HIV reactive. suggests that it is probably not helpful perform antibody testing HIV-exposed non-breastfed who have met virologic criteria exclude infection first year life.

Abstract Aims Antimicrobial resistance in P. aeruginosa represents a major global challenge public and veterinary health, particularly from One Health perspective. This study aimed to investigate antimicrobial resistance, the presence of virulence genes, genetic diversity isolates diverse sources. Methods Results The utilized susceptibility testing, genomic analysis for multilocus sequence typing characterize total 737 were collected humans, domestic animals, aquatic environments northern...

São apresentados dois casos de paracoccidioidomicose, um em paciente com a síndrome da imunodeficiência adquirida e o outro infecção pelo HIV. Trata-se dos primeiros relatos que esta associação é descrita na literatura. No primeiro, micose se evidenciou durante acompanhamento AIDS, passou apresentar hépato-esplenomegalia febre elevada. A ecografia, radiografia simples tomografia computadorizada do abdômen, demonstraram nódulos sólidos, alguns calcificados, no parênquima esplénico. punção...

ABSTRACT A striking increase in the numbers of cultures positive for Mycobacterium tuberculosis was noticed a mycobacterial reference laboratory Campinas, Sao Paulo State, Brazil, May 1995. contaminated bronchoscope suspected cause increase. All 91 M. isolates grown from samples patients between 8 and 18 July 1995 were characterized by spoligotyping IS 6110 fingerprinting. Sixty-one had identical spoligotype patterns, pattern arbitrarily designated S36. The 61 specimens containing these been...

Many Escherichia coli strains harbour astA, which is the gene encoding enteroaggregative E. heat-stable enterotoxin (EAST1). This embedded in a putative transposase (ORF1) and presents polymorphism diarrheagenic strains. Although astA orf1 are detected extraintestinal strains, little known about differential transcription this pathotype. In present work, from humans (ExPEC - Extraintestinal Pathogenic coli) poultry (APEC Avian were assayed to verify presence of astA/orf1 possible...

The prevalence of resistance to imipenem and ceftazidime among Pseudomonas aeruginosa isolates is increasing worldwide.Risk factors for nosocomial recovery (defined as the finding culture-positive after hospital admission) imipenem-resistant P. (IRPA) ceftazidime-resistant (CRPA) were determined.Two separate case-control studies conducted. Control subjects matched case patients (ratio, 2:1) on basis admission same ward at time patient. Variables investigated included demographic...

The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the status patients undergoing liver transplant. β-herpesviruses, including 6, are ubiquitous among populations. Active common after transplantation, possibly induced facilitated by allograft rejection immunosuppressive therapy. Both viruses affect success transplant procedure. Thirty submitted at...

OBJECTIVE:To determine the incidence of Mycobacterium tuberculosis complex and non-tuberculous mycobacterial isolates in routine setting a large general hospital using an "in-house" multiplex polymerase chain reaction method to establish paradigm for definitive identification mycobacteria isolated semi-automated equipment.METHODS: Established tests, including restriction enzyme analysis, PNB, NAP inhibition tests as gold standard, showed 100% agreement with IS6110/hsp65 when used identify...

Syphilis is a reemergent infection worldwide. There has been steady increase in incidence across many population groups the last decades, requiring public health authorities' attention. We report case of 67-year-old man with neurosyphilis who presented abrupt clinical ophthalmic changes and sensorineural hearing loss. The neuroradiological investigation demonstrated cranial nerves vascular involvement, detected on high-resolution vessel wall imaging brain magnetic resonance imaging. CSF...

OBJETIVO: A micobacteriose é frequentemente diagnosticada entre pacientes infectados pelo HIV. Em Moçambique, onde apenas um pequeno número de encontra-se sob tratamento anti-retroviral, e a tuberculose tem alta prevalência, existe necessidade melhor caracterização destes agentes bacterianos, em nível espécie, bem como se caracterizar os padrões resistência às drogas antituberculosas. MÉTODOS: uma coorte 503 indivíduos HIV positivos suspeitos pulmonar, 320 apresentaram positividade para...

One hundred and fifty-one methicillin-resistant z (MRSA) strains have been isolated from patients admitted in tertiary care hospitals two metropolitan areas (Campinas City Ribeirão Preto City) the southeast region of Brazil analyzed through PCR-based techniques [(PCR amplification spa, coa, housekeeping genes (arcC, aroE, gmk, pta, tpi, yqiL)] further restriction fragment typing coa genes. The heterogeneity spa gene was determined directly by agarose gel electrophoresis migration. results...

Lymphocytic interstitial pneumonitis (LIP) was classically described by Carrington and Leibow in 1966 non-AIDS patients (1). This pathological description of diffuse lymphocytic infiltration the interstitium later associated with various autoimmune disorders such as Sjogren's syndrome, thyroiditis others (2). Since beginning AIDS epidemic several reports common finding LIP children infected human immume deficiency virus (HIV) (3). Less commonly, reported adults, mostly Haitians, or...

The etiology of pulmonary infections in HIV patients is determined by several variables including geographic region and availability antiretroviral therapy. A cross-sectional prospective study was conducted from 2012 to 2016 evaluate the occurrence fungal infection HIV-patients hospitalized due infections. Patients' serums were tested for (1–3)-β-D-Glugan, galactomannan, lactate dehydrogenase. association among analyzed univariate multivariate regression analysis. 60 included study....

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Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present local prevalent strains Mycobacterium tuberculosis, distribution and clustering strains. DNA fingerprinting was performed on sample M. tuberculosis isolates from patients with AFB smear-positive pulmonary TB, at hospital Brazil. The profiles were analyzed compared database Houston Initiative, Houston, US. Seventy-six fingerprints...

As doenças respiratórias constituem expressiva parcela da morbidade na população cidade de Ribeirão Preto, como comprovam alguns autores. Em particular, as pulmonares crônicas não-específicas motivam número grande internações a cada ano. Tais podem ser detectadas através sintomas relatados em entrevistas pessoais, quando se usa um questionário padronizado. uma amostra 3.353 indivíduos mais 3 anos idade, correspondendo 878 domicílios visitados, foi feito levantamento respiratórios, do...

Abstract This study evaluated the IS6220-RFLP patterns of 109 Mycobacterium tuberculosis isolates patients with HIV cared for at a Brazilian university hospital. Thirteen clusters involving 35 (32.1%) individuals were identified. Nosocomial transmission was possible in 5 cases. Strategies to prevent M. should be implemented hospitals developing countries.

FIGURE 1: (A) Chest computed tomography, pulmonary window, axial, showing small areas of consolidation in the peripheral upper segment left lower lobe (black arrow), a typical finding patients with COVID-19 pneumonia; (B and C) axial chest tomography contrast mediastinal window.Large filling defects adherent to walls both right main arteries before their bifurcation (white arrows), an appearance compatible thromboembolism.