A5009614864- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Nutrition and Health in Aging
- Telomeres, Telomerase, and Senescence
- Parvovirus B19 Infection Studies
- Frailty in Older Adults
- Laser Applications in Dentistry and Medicine
- Mosquito-borne diseases and control
- Bacterial Identification and Susceptibility Testing
- Genetics, Aging, and Longevity in Model Organisms
- Body Composition Measurement Techniques
- Electrochemical sensors and biosensors
- Electrochemical Analysis and Applications
- Antimicrobial Resistance in Staphylococcus
- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- Infection Control in Healthcare
- Infective Endocarditis Diagnosis and Management
- Nutritional Studies and Diet
- Toxoplasma gondii Research Studies
- Phytochemicals and Antioxidant Activities
- HIV/AIDS oral health manifestations
- Dental Trauma and Treatments
- Monoclonal and Polyclonal Antibodies Research
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2017-2024
Universidade Estadual de Campinas (UNICAMP)
2007-2012
Centro Universitário Herminio Ometto de Araras
2008-2012
Immunosenescence is an age-related reduction of immune system activity that can be associated with frailty. This study aimed to compare cytomegalovirus (CMV) and Epstein–Barr virus (EBV) reactivations (based on viremias) between young elderly women who had a chronic CMV and/or EBV infection (i.e., IgG+ serostatus) without acute IgM− serostatus), among the group categorized according frailty status. DNA was extracted from plasma using standard protocols serostatus determined by enzyme-linked...
Certain cut-off points for sarcopenia screening and diagnosis are arbitrary based on European populations, with normative references often obtained from healthy young adults. Although respiratory skeletal muscle strength tests represent low-cost clinical measures commonly performed in practice by health professionals, a gap remains regarding whether adequate sensitive screening. This study aimed to verify the value of handgrip as possible discriminators identify establish community-dwelling,...
We examined associations between adverse childhood experiences (ACEs) and shorter telomere length (TL) in 83 older women, including 42 women with less than secondary education 41 or more a city of Northeast Brazil, region substantial socioeconomic inequalities. The low sample was selected from representative survey at local neighborhood health centers, while the high group consisted convenience recruited by advertising community centers affiliated university. Relative leukocyte TL measured...
The aim of this study was to simultaneously monitoring cytomegalovirus and human herpesvirus 6 active infections using nested-polymerase chain reaction and, together with clinical findings, follow the status patients undergoing liver transplant. β-herpesviruses, including 6, are ubiquitous among populations. Active common after transplantation, possibly induced facilitated by allograft rejection immunosuppressive therapy. Both viruses affect success transplant procedure. Thirty submitted at...
Thomasini RL, Bonon SH, Durante P, Costa SCB. Correlation of cytomegalovirus and human herpesvirus 7 with CD3 + CD4 cells in chronic periodontitis patients. J Periodont Res 2012; 47: 114–120. © 2011 John Wiley & Sons A/S Background Objective: Human is an inflammatory process characterized by dense accumulation immune the periodontal tissue. The can lead to loss teeth patient pathogenesis this disease not completely known. This study tested hypothesis that periodontitis‐affected sites...
Human herpesvirus-6 and -7 (HHV-6, HHV-7) remain latent after primary infection can reactivate transplantation. HHV-6 active has been related to some clinical manifestation, but the role of HHV-7 remains unclear. The significance DNAemia is not completely known immune response against poorly studied in In this study, we investigated liver transplant recipients evaluated immunoglobulin (Ig) G IgM HHV-7. A total 22 adult were followed up for 90 days. was detected by nested polymerase chain...
Human herpesvirus (HHV)-6, HHV-7, and cytomegalovirus (CMV) that remain latent after primary infection can be reactivated during immunosuppression following organ transplantation in liver transplant recipients. The aim of this study was to monitor active infections for HHV-6, CMV among adult recipients using antigenemia detected by an immunoperoxidase staining. Twenty-eight patients were monitored blood samples obtained at the time transplantation, as well weekly first month once a 6 months....
Introduction: Photobiomodulation with low-level laser treatment can enhance bone formation by stimulating the cell division of osteoblasts and increasing amount protein deposition, thus encouraging new bone. The aim this study was to evaluate effects photobiomodulation a on proliferation gene expression related calcium signaling in human osteoblasts. Methods: Osteoblastic lines hFOB1.19 lineage, osteoblasts, were grown assigned into two groups, control (C; n=78 cultured wells) (L; n=6 per...
This study reports, for the first time, chemical composition and biological activity of essential oil from Brazilian Cymbopogon densiflorus flowers. Thirty compounds were identified by gas chromatography coupled with mass spectrometry (GC/MS), corresponding to 95 % total an chromatogram obtained chromatography-flame ionization detection (GC-FID ). The was mainly composed cis-p-mentha-1(7),8-dien-2-ol (26 %), trans-p-mentha-1(7),8-dien-2-ol (18 trans-p-mentha-2,8-dien-1-ol (13 %) limonene (9...
INTRODUCTION Molecular techniques for the detection of pathogens have been shown to be effective diagnostic tools with high sensitivity and short turnaround times. METHODS This study compared five Staphylococcus aureus DNA extraction methods by polymerase chain reaction. RESULTS: The concentration purity extracted showed that did not yield significant quality. However, most protocols yielded 100% positivity, even low concentrations. CONCLUSIONS: Although one protocol seemed more efficient...
Background The most of the hepatitis C‐infected patients remain undiagnosed until they develop severe liver damage or submitted for serological screening. Objective To evaluate a recombinant multiepitope protein detection IgG anti‐hepatitis C virus. Method: A synthetic gene was cloned, expressed in Escherichia coli , and purified. Human serum panel consisted 88 positives (20 HCV genotyped) 376 negatives C, 6 human acquired immunodeficiency virus, syphilis positives, B were tested by...
Purpose: To evaluate osteocalcin gene and protein expression in vitro an vivo model of ostectomy. Methods: Twenty Wistar rats were assigned into two groups A (n=10, laser) B control). Ostectomy was performed the femur diaphysis; twenty fragments removed, composed named as (A B) cultivated CO2 atmosphere for thirteen days. Low-level laser irradiation (in vitro) by GaAlAs device (λ=808 nm, dose 2J/cm2, power 200mW, density 0.2W/cm2, total energy 1.25J, spot diameter 0.02mm) 5 seconds, at one...
Leukocyte telomere length in the elderly has been positively associated with healthy living and physical activity. Factors that interfere shortening are similar to those may be decreasing functional capacity. To investigate relationship between mean leukocyte capacity community-dwelling individuals, this is an observational, cross sectional, multicentric study conducted Brazilian patients. Sample characterization was performed using a sociodemographic clinical questionnaire. Telomere...
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections the host. The significance viral DNA detected leukocytes by PCR unclear cross-reaction serological tests may occur. This study aimed to evaluate nested-PCR detect liver recipients compared healthy individuals. was performed on sera 53 volunteers 29 recipients. In volunteers, 28.3% 0% serum. 48.2% Nested-PCR extracted from latent...
This study assessed whether telomere length is related to chronic conditions, cardiovascular risk factors, and inflammation in women aged 65 74 from Northeast Brazil. Participants were selected two sources, a representative sample of the International Mobility Aging Study (n = 57) convenience 49) recruited at senior centers. Leukocyte was measured by quantitative polymerase chain reaction blood samples 83 women. Natural log-transformed telomere/single copy gene ratio used as dependent...
Despite current literature pointing to a link between shortened telomeres and aging, chronic diseases, geriatric syndromes, the precise implications of this connection remain unclear. The aim exploratory, cross-sectional, observational study was investigate association relative telomere length (RTL) peripheral blood leukocyte subtypes (mononuclear cells granulocytes) physical performance using Short Physical Performance Battery (SPPB) in older adults. A cohort 95 participants recruited,...
INTRODUCTION: Human herpesvirus (HHV)-7 establishes a latent infection during the lifetime of host and can reactivate after primary infection, leading to lytic replication in immunosuppressed patients. METHODS: This study aimed develop an enzyme-linked immunosorbent assay (ELISA) identify HHV-7 serum antibodies compare its performance with that indirect immunofluorescence (IFA). RESULTS: Serum samples (n=102) were tested by IgG-IFA ELISA. IFA ELISA showed IgG-positive results 77 73 samples,...
Abstract Leukocyte telomere length in the older adults has been associated with cell aging and relationship frailty syndrome is unclear. Factors that interfere shortening could be similar to those linked frailty. The aims of this study were compare relative telomeric (RTL) DNA extracted from whole blood leukocytes, mononuclear cells granulocytes community-dwelling classified as frail, non-frail, pre-frail, evaluate if there a between subpopulations subgroups adults. This an observational,...