A5027192420- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Leptospirosis research and findings
- Public Health in Brazil
- Central Venous Catheters and Hemodialysis
- Healthcare Regulation
- Meta-analysis and systematic reviews
- Antibiotics Pharmacokinetics and Efficacy
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Phytochemistry Medicinal Plant Applications
- Bacterial Identification and Susceptibility Testing
- Medical Device Sterilization and Disinfection
- Diphtheria, Corynebacterium, and Tetanus
- Salmonella and Campylobacter epidemiology
- Insect and Arachnid Ecology and Behavior
- Hip and Femur Fractures
- Pituitary Gland Disorders and Treatments
- Legionella and Acanthamoeba research
- Bacterial biofilms and quorum sensing
- Microscopic Colitis
Universidade Estadual Paulista (Unesp)
1987-2021
Instituto Adolfo Lutz
1969
Avian pathogenic Escherichia coli (APEC) strains belong to a category that is associated with colibacillosis, serious illness in the poultry industry worldwide. Additionally, some APEC groups have recently been described as potential zoonotic agents. In this work, we compared extraintestinal E. (ExPEC) isolated from clinical cases of humans extra-intestinal diseases such urinary tract infections (UTI) and bacteremia. PCR results showed genes usually found ColV plasmid (tsh, iucA, iss, hlyF)...
Species identification and antifungal susceptibility tests were carried out on 212 Candida isolates obtained from bloodstream infections, urinary tract infections dialysis-associated peritonitis, cases attended at a Brazilian public tertiary hospital January 1998 to 2005.Candida albicans represented 33% of the isolates, parapsilosis 31.1%, tropicalis 17.9%,Candida glabrata 11.8%, others species 6.2%. In blood culture, C. was most frequently encountered (48%). The resistance levels azoles...
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. Following optimization logistics that shipping isolates as thermolysates in ambient conditions, project assembled diverse 10,419 from low- middle-income countries. The genomes were sequenced using LITE library...
The aim of the present study was to investigate prevalence D iarrheagenic E scherichia coli ( DEC ) pathotypes, a leading cause diarrhea worldwide, among diarrheal and healthy children, up 5 years age, living in city B otucatu, S ão P aulo, razil. , investigated by PCR detection virulence factor‐encoding genes associated with distinct isolated from 18.0% patients, 19.0% controls, enteroaggregative . EAEC ), most frequent pathotype, being detected equal proportion between patients controls...
Dissemination of carbapenem-resistant Acinetobacter baumannii is currently one the priority themes discussed around world, including in Brazil, where this pathogen considered endemic. A total 107 A. (CRAB) isolates were collected from patients with bacteraemia attended at a teaching hospital Brazil 2008 to 2014. From these samples, 104 (97.2%) carried blaOXA-23-like, all them associated ISAba1 The blaOXA-231 (1.9%) and blaOXA-72 (0.9%) genes also detected low frequencies. All susceptible...
Over the last decades, there have been important changes in epidemiology of Candida infections. In recent years, species emerged as causes invasive infections mainly among immunocompromised patients. This study analyzed spp. isolates and compared frequency biofilm production different sources isolation: blood, urine, vulvovaginal secretions peritoneal dialysis fluid. Biofilm was quantified 327 obtained from patients attended at a Brazilian tertiary public hospital (Botucatu, Sao Paulo). C....
Foram estudados 117 pacientes com fraturas expostas, submetidos a protocolo para identificação do paciente e características trauma, internados tratamento cirúrgico, durante um período de dois anos. A avaliação microbiológica da ferida foi realizada em 45 antes desbridamento predomínio dos germes gram positivos. antibioticoterapia utilizada profilaticamente todos os pacientes, podendo, no entanto, ser melhor padronizada. O perfil maioria amostra foi: sexo masculino, branco, casado, idade...
Acinetobacter baumannii is the main species of genus; however, non-baumannii (NBA) causing infections have been described for past years, as well antimicrobial resistance. In this study, we describe occurrence two multidrug-resistant (MDR) IMP-1-producing bereziniae isolates recovered from bloodstream in different patients but same intensive care unit among 134 carbapenem-resistant screened. Antimicrobial susceptibility testing revealed resistance to carbapenems, extended spectrum, and...
Phenotypic and genotypic SPM IMP metallo-β-lactamases (MBL) detection also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples either blaSPM or blaIMP genes, while best phenotypic assay (ceftazidime substrate mercaptopropionic acid inhibitor) 13 these samples. Imipenem, MICs higher for MBL compared...
Objective To evaluate the effectiveness of Gram stain in initial diagnosis etiologic agent peritonitis continuous ambulatory peritoneal dialysis (CAPD). Design Retrospective study analyzing sensitivity (S), specificity (SS), positive predictive value (+PV), and negative (-PV) relating to results cultures 149 episodes CAPD. The data were analyzed two studies. In first, only cases with detection a single by taken (Study 1). second, agents evaluated 2). Setting Dialysis Unit Laboratory...
Para se detectar diferenças imuno-antigênicas entre 8 amostras de P. brasiliensis isoladas diferentes áreas endêmicas (Botucatu: Pb 1, 2 e 3; São Paulo: Pb: 18, 192 265; Venezuela: 9 73), esutdaram-se: 1. A reatividade antigênica cada amostra nas reações imunofluorescência indireta (II) imunodifusão dupla em gel agar (ID) contra painel 20 soros controles positivos para paracoccidioidomicose; 2. capacidade induzir resposta imune humoral (medida por imunodifusão) celular pelo teste coxim...
Peritonitis remains the main complication of peritoneal dialysis (PD). Antimicrobial therapy may be threatened by development bacterial resistance, demanding continuous surveillance infectious agents to improve empirical treatment. The aims this study were evaluate causing peritonitis and their antimicrobial susceptibility in patients undergone PD a Brazilian center, between 1993 2013. Strains recovered from fluid, identified usual methods submitted Etest susceptibility. A total 400 strains...
Phenotypic and genotypic SPM IMP metallo-β-lactamases (MBL) detection also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples either blaSPM or blaIMP genes, while best phenotypic assay (ceftazidime substrate mercaptopropionic acid inhibitor) 13 these samples. Imipenem, MICs higher for MBL compared...
Objective: to compare the efficacy of 80% (w/v) alcohol, rubbed for 30 and 60 seconds, in manual processing stainless-steel wash bowls, after cleaning with running water neutral detergent. Method: experimental study conducted a hospital state São Paulo, Brazil, on 50 bowls randomly divided into two groups 25 each interventions seconds rubbing alcohol. Results: based microbiological analyses collected, before both groups, partial disinfectant was observed even when extending time. In there...
The experimental model of paracoccidioidomycosis induced in mice by the intravenous injection yeast-forms P. brasiliensis (Bt2 strain; 1 x 10(6) viable fungi/animal) was used to evaluate sequentially 2, 4, 8, 16 and 20 weeks after inoculation: 1. presence immunoglobulins C3 pulmonary granuloma-ta, direct immunofluorescence; 2. humoral (immunodiffusion test) cellular (footpad sweeling immune response; 3. histopathology lesions. cell-immune response positive since week showing a transitory...
São relatados dois casos humanos de leptospirose por L. javanica, oriundos Bôca do Acre, Amazonas, comprovados através das provas sôro-aglutinação.