A5034807742- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Mycotoxins in Agriculture and Food
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Microbial Fuel Cells and Bioremediation
- Probiotics and Fermented Foods
- Wastewater Treatment and Nitrogen Removal
- COVID-19 Clinical Research Studies
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Electrospun Nanofibers in Biomedical Applications
- Antibiotic Resistance in Bacteria
- Advanced Sensor and Energy Harvesting Materials
- GABA and Rice Research
- Listeria monocytogenes in Food Safety
- Metalloenzymes and iron-sulfur proteins
- Microbial infections and disease research
- COVID-19 epidemiological studies
- COVID-19 and healthcare impacts
- Microbial bioremediation and biosurfactants
- Ammonia Synthesis and Nitrogen Reduction
- COVID-19 diagnosis using AI
Ambulance Service of New South Wales
2025
Edinburgh Napier University
2023
Earlham Institute
2020-2022
Norwich Research Park
2009-2021
Genomics (United Kingdom)
2020
Quadram Institute
2009-2016
University of Würzburg
2012
University of East Anglia
1999-2006
John Innes Centre
2004-2005
Electro-blown spinning (EBS) is an emergent hybridized nanofibers formation technology. Recently, there has been a great interest in introducing this novel method for producing sub-micron, and into several applications. For the first time, comprehensive paper provides detailed review EBS process, including working principle, operation parameters, materials, setup modifications, various manufacturing process which combines between solution-blown (SBS) electrospinning driving forces. The can...
Background and Aims Both deoxynivalenol (DON) nontyphoidal salmonellosis are emerging threats with possible hazardous effects on both human animal health. The objective of this study was to examine whether DON at low but relevant concentrations interacts the intestinal inflammation induced by Salmonella Typhimurium. Methodology By using a porcine ileal loop model, we investigated intake early inflammatory response Results A significant higher expression IL-12 TNFα clear potentiation IL-1β,...
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. Following optimization logistics that shipping isolates as thermolysates in ambient conditions, project assembled diverse 10,419 from low- middle-income countries. The genomes were sequenced using LITE library...
Salmonella Typhimurium infections in pigs often result the development of carriers that intermittently excrete very low numbers. During periods stress, for example transport to slaughterhouse, recrudescence may occur, but mechanism this stress related is poorly understood. Therefore, aim present study was determine role hormone cortisol by pigs. We showed a 24 h feed withdrawal increases intestinal load pigs, which correlated with increased serum levels. A second vivo trial demonstrated can...
Abstract Background There is a high prevalence of COVID-19 in university-age students, who are returning to campuses. little evidence regarding the feasibility universal, asymptomatic testing help control outbreaks this population. This study aimed pilot mass on university research park, assess and acceptability scaling up all staff students. Methods was cross-sectional park East England. All students (5625) were eligible participate. participants offered four PCR swabs, which they...
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A proportion of patients due to anatomical variation do not receive chest compressions over the left ventricle. Transoesophageal echocardiography (TOE) has potential impact survival rates by identifying area maximal compression (AMC), potentially improving forward flow and systemic perfusion in cardiac arrest. There is a paucity data regarding use TOE during out hospital arrest (OHCA) pre-hospital setting, with most coming from studies performed hospital. We therefore set retrospectively...
Summary Methylmenaquinol : fumarate reductase (Mfr) is a newly recognized type of present in some ε‐proteobacteria, where the active site subunit (MfrA) localized periplasm, but for which physiological role has not been identified. We show that Campylobacter jejuni mfrABE operon transcribed from single promoter, with mfrA gene preceded by small open reading‐frame ( mfrX ) encoding C. ‐specific polypeptide unknown function. The growth characteristics and enzyme activities mutants menaquinol A...
The mycotoxin T-2 toxin and Salmonella Typhimurium infections pose a significant threat to human animal health. Interactions between both agents may result in different outcome of the infection. Therefore, aim presented study was investigate effects low relevant concentrations on course infection pigs. We showed that presence 15 83 μg per kg feed significantly decreased amount bacteria present cecum contents, tendency reduced colonization jejunum, ileum, cecum, colon contents noticed. In...
Rhodobacter sphaeroides strain 2.4.1 is a widely studied bacterium that has recently been shown to cleave the abundant marine anti-stress molecule dimethylsulfoniopropionate (DMSP) into acrylate plus gaseous dimethyl sulfide. It does so by using lyase encoded dddL, promoter-distal gene of three-gene operon, acuR-acuI-dddL. Transcription operon was enhanced when cells were pre-grown with substrate DMSP, but this induction indirect, and requires conversion DMSP product acrylate, bona fide...
The expression of genes within Salmonella Pathogenicity Islands 1 and 2 (SPI1, SPI2) is required to facilitate invasion intracellular replication respectively S. Typhimurium in host cell lines. Control their complex occurs via a variety factors operating at transcriptional post-transcriptional levels response the environmental stimuli found host. Several that modulate SPI1 SPI2 are involved redistribution or modification RNA polymerase (RNAP) specificity. These include bacterial alarmone,...
Abstract Host stress is well known to result in flare-ups of many bacterial, viral and parasitic infections. The mechanism by which host exploited increase pathogen loads, poorly understood. Here we show that Salmonella enterica subspecies serovar Typhimurium employs a dedicated mechanism, driven the scsA gene, respond hormone cortisol. Through this cortisol increases proliferation inside macrophages, resulting increased intestinal infection loads DBA/2J mice. ScsA directs overall virulence...
ABSTRACT The transcription factor NNR from Paracoccus denitrificans was expressed in a strain of Escherichia coli carrying plasmid-borne fusion the melR promoter to lacZ , with consensus FNR-binding site 41.5 bp upstream start site. This activated by under anaerobic growth conditions media containing nitrate, nitrite, or NO + donor sodium nitroprusside. Activation nitrate abolished mutation molybdenum cofactor biosynthesis pathway, indicating requirement for reductase activity. modulated...
The necessity to respond the level of fixed nitrogen and external oxygen concentrations provide sufficient energy for fixation imposes common regulatory principles amongst diazotrophs. NifL–NifA system in Azotobacter vinelandii integrates signals redox, fixed-nitrogen carbon status regulate nif transcription. Multidomain signalling interactions between NifL NifA are modulated by redox changes, ligand binding interaction with signal-transduction protein GlnK. Under adverse conditions (excess...
The nitrite reductase and nitric oxide regulator (NNR) from Paracoccus denitrificans activates transcription in response to (NO). mechanism of NO sensing has not been elucidated for NNR, or any its orthologues the FNR/CRP family transcriptional regulators. Using regulated expression nnr gene Escherichia coli, evidence now obtained indicate that activation NNR by does require de novo synthesis polypeptide. In anaerobic cultures, is inactivated slowly following removal source NO. contrast,...
Abstract Background Invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium ( S . Typhimurium) requires expression the extracellular virulence gene programme (ST EX ), activation which is dependent on signalling molecule guanosine tetraphosphate (ppGpp). Recently, next-generation transcriptomics (RNA-seq) has revealed unexpected complexity bacterial transcriptomes and in this report we use differential RNA sequencing (dRNA-seq) to define high-resolution...
We have used differential RNA-seq (dRNA-seq) to characterise the transcriptomic architecture of S. Typhimurium SL1344, and its dependence on bacterial alarmone, guanosine tetraphosphate (ppGpp) during late stationary phase, (LSP). Under LSP conditions we were able identify transcriptional start sites (TSSs) for 53% open reading frames (ORFs) discovered 282 candidate non-coding RNAs (ncRNAs). The mapping TSSs enabled a detailed comparison with previous dRNA-seq study early phase (ESP) SL1344...
Salmonella enterica serovar Enteritidis has developed the potential to contaminate table eggs internally, by colonization of chicken reproductive tract and internalization in forming egg. The serotype mechanisms colonize oviduct more successfully than other serotypes. Until now, strategies exploited do so have remained largely unknown. For that reason, a microarray-based transposon library screen was used identify genes are essential for persistence inside primary gland cells vitro vivo. A...
Eggs contaminated with Salmonella Enteritidis are an important source of human foodborne infections.Salmonella is able to contaminate egg white during formation the within chicken oviduct, and it has developed strategies withstand antimicrobial properties survive in this hostile environment.The mechanisms involved persistence likely be complex.To address issue, a microarray-based transposon library screen was performed identify genes necessary for survival at body temperature.The majority...
Abstract We have developed an efficient and inexpensive pipeline for streamlining large-scale collection genome sequencing of bacterial isolates. Evaluation this method involved a worldwide research collaboration focused on the model organism Salmonella enterica , 10KSG consortium. By optimising logistics that collected isolates as thermolysates, permitting shipment in ambient conditions, project assembled diverse 10,419 clinical environmental from low- middle-income countries less than one...
The Paracoccus denitrificans transcription factor FnrP has been characterized using artificial FNR-dependent promoter-lacZ fusion plasmids in Escherichia coli. can activate both class I and II promoters response to anoxia but shows a marked preference for the promoter, where FNR binding site is centered at -41.5 with respect start site. was found be inactive an iscS mutant vivo, demonstrating requirement cysteine desulfurase activity assemble iron-sulfur cluster FnrP. Accordingly, could...
ABSTRACT A pleiotropic mutant of Paracoccus denitrificans , which has a severe defect that affects its anaerobic growth when either nitrate, nitrite, or nitrous oxide is used as the terminal electron acceptor and also unable to use ethanolamine carbon energy source for aerobic growth, was isolated. This phenotype expressed only during on minimal media can be reversed by addition cobalamin (vitamin B 12 ) cobinamide rich media. Sequence analysis revealed mutation causing this in gene...