A5075047605- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- Metalloenzymes and iron-sulfur proteins
- Bacterial Genetics and Biotechnology
- Microbial Metabolism and Applications
- Microbial Fuel Cells and Bioremediation
- Legume Nitrogen Fixing Symbiosis
- Insect and Pesticide Research
- Nitric Oxide and Endothelin Effects
- Plant Disease Resistance and Genetics
- Microbial Community Ecology and Physiology
- Synthetic Organic Chemistry Methods
- Mycorrhizal Fungi and Plant Interactions
- Plant biochemistry and biosynthesis
- Animal Behavior and Reproduction
- Carbohydrate Chemistry and Synthesis
- Insect behavior and control techniques
- Metal-Catalyzed Oxygenation Mechanisms
- Chemical Synthesis and Analysis
- Photosynthetic Processes and Mechanisms
John Innes Centre
2005-2025
Norwich Research Park
2014-2024
University of East Anglia
2012-2022
Norwich University
2017
Rigshospitalet
2017
University of St Andrews
2015
Northumbria University
2008
Molina Center for Energy and the Environment
2005
University of Southampton
2000
Abstract Background Attine ants live in an intensely studied tripartite mutualism with the fungus Leucoagaricus gongylophorus , which provides food to ants, and antibiotic-producing actinomycete bacteria. One hypothesis suggests that bacteria from genus Pseudonocardia are sole, co-evolved mutualists of attine transmitted vertically by queens. A recent study identified a -produced antifungal, named dentigerumycin, associated lower Apterostigma dentigerum consistent idea make novel...
Attine ants are dependent on a cultivated fungus for food and use antibiotics produced by symbiotic Actinobacteria as weedkillers in their gardens. Actinobacterial species belonging to the genera Pseudonocardia, Streptomyces Amycolatopsis have been isolated from attine ant nests shown confer protection against range of microfungal weeds. In previous work higher Acromyrmex octospinosus we strain that produces candicidin, consistent with another report Streptomyces-produced candicidin...
The regulatory protein NsrR, a member of the Rrf2 family transcription repressors, is specifically dedicated to sensing nitric oxide (NO) in variety pathogenic and non-pathogenic bacteria. It has been proposed that NO directly modulates NsrR activity by interacting with predicted [Fe-S] cluster protein, but no experimental evidence published support this hypothesis. Here we report purification from obligate aerobe Streptomyces coelicolor. We demonstrate using UV-visible, near UV CD EPR...
Ant pharming: antibacterial polyketides from plant-ant associated bacteria.
Abstract Acromyrmex leafcutter ants form a mutually beneficial symbiosis with the fungus Leucoagaricus gongylophorus and Pseudonocardia bacteria. Both are vertically transmitted actively maintained by ants. The garden is manured freshly cut leaves provides sole food for ant larvae, while cultures reared on ant-cuticle make antifungal metabolites to help protect cultivar against disease. If left unchecked, specialized parasitic Escovopsis fungi can overrun lead colony collapse. We report that...
The vertebrate gut microbiome (GM) can vary substantially across individuals within the same natural population. Although there is evidence linking GM to health in captive animals, very little known about consequences of variation for host fitness wild. Here, we explore relationship between faecal diversity, body condition, and survival using data from long-term study a discrete population Seychelles warbler (Acrocephalus sechellensis) on Cousin Island. To our knowledge, this first time that...
Summary Vancomycin is the front‐line therapy for treating problematic infections caused by methicillin‐resistant Staphylococcus aureus (MRSA), and spread of vancomycin resistance an acute problem. blocks cross‐linking between peptidoglycan intermediates binding to d ‐Ala‐ ‐Ala termini bacterial cell wall precursors, which are substrate transglycosylase/transpeptidase. We have characterized a cluster seven genes ( vanSRJKHAX ) in Streptomyces coelicolor that confers inducible, high‐level...
Summary We took advantage of the vancomycin‐dependent phenotype Streptomyces coelicolor femX null mutants to isolate a collection spontaneous, drug‐independent suppressor that expressed vancomycin‐resistance ( van ) genes constitutively. All mutations were in vanS but, unexpectedly, many predicted be loss‐of‐function mutations. Confirming this interpretation, constructed deletion mutation also resulted constitutive expression genes, suggesting VanS negatively regulated VanR function absence...
Significance The evolutionary significance of assortative mating by diet, mediated gut bacteria is a puzzle, but it has had huge impact and provided keystone to support increasing interest in the “holobiome.” However, species such as Drosophila melanogaster that have flexible microbiomes, any reproductive isolation specific host diets can only be transient. Here, we replicated extended tests this idea. Despite differences failed recover previously observed patterns nonrandom found no...
Abstract NsrR from Streptomyces coelicolor ( Sc) regulates the expression of three genes through progressive degradation its [4Fe–4S] cluster on nitric oxide (NO) exposure. We report 1.95 Å resolution crystal structure dimeric holo-ScNsrR and show that is coordinated by invariant Cys residues one monomer and, unexpectedly, Asp8 other. A cavity map suggests NO displaces as a ligand while D8A D8C variants remain sensitive, DNA binding affected. structural comparison with an apo-IscR-DNA...
We must reduce reliance on agrochemicals, and there is increasing interest in using bacterial strains to promote plant growth protect against disease. Our study follows up reports that Arabidopsis thaliana specifically recruits Streptomyces bacteria its roots. test the hypotheses they offer benefits their A. hosts isolated from these plants might be used as probiotics. roots genome sequenced five phylogenetically distinct strains. Genome mining bioassays indicated all have growth-promoting...
ABSTRACT The Escherichia coli norVW genes encode a flavorubredoxin and NADH:(flavo)rubredoxin reductase, respectively, which are involved in nitric oxide detoxification under anaerobic growth conditions. Here it is shown that the also have role protection against reactive nitrogen intermediates generated from nitroprusside. Transcription norV promoter activated by presence of nitroprusside medium; activation requires product divergently transcribed regulatory gene, norR .
Abstract: Background/Aims: Resolution of liver fibrosis is possible but the identity matrix metalloproteinases (MMPs) which degrade accumulated collagens uncertain. We examined MMP‐2 and MMP‐14 expression in established resolving to assess their role resolution fibrosis. Methods: MMP tissue inhibitor metalloproteinase (TIMP)‐2 extracts was by ribonuclease protection assay, Western blotting gelatin zymography. activity 14 C degradation. Results: In human cirrhotic liver, mRNA increased...