A5074148045- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Immune Cell Function and Interaction
- Pancreatic function and diabetes
- Diabetes Treatment and Management
- PI3K/AKT/mTOR signaling in cancer
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Pluripotent Stem Cells Research
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
Royal Netherlands Academy of Arts and Sciences
2021-2025
University Medical Center Utrecht
2023-2025
Oncode Institute
2025
Roche (Switzerland)
2024
University of Applied Sciences Utrecht
2017
Tumors arise from uncontrolled cell proliferation driven by mutations in genes that regulate stem renewal and differentiation. Intestinal tumors, however, retain some hierarchical organization, maintaining both cancer cells (CSCs) differentiated (CDCs). This heterogeneity, coupled with cellular plasticity enabling CDCs to revert CSCs, contributes therapy resistance relapse. Using genetically encoded fluorescent reporters human tumor organoids, combined our machine-learning-based tracker,...
Optimization of CRISPR/Cas9-mediated genome engineering has resulted in base editors that hold promise for mutation repair and disease modeling. Here, we demonstrate the application generation complex tumor models human ASC-derived organoids. First show efficacy cytosine adenine modeling CTNNB1 hot-spot mutations hepatocyte Next, use C > T to insert nonsense PTEN endometrial organoids tumorigenicity even heterozygous state. Moreover, drug sensitivity assays on harboring either or PIK3CA...
BEST4/CA7+ cells of the human intestine were recently identified by single-cell RNA sequencing. While their gene expression profile predicts a role in electrolyte balance, cell function has not been explored experimentally owing to absence mice and paucity vitro models. Here, we establish protocol that allows emergence intestinal organoids. Differentiation requires activation Notch signaling transcription factor SPIB. numbers strongly increase response cytokine interferon-γ, supporting...
Enteroendocrine cells (EECs) are gut epithelial that respond to intestinal contents by secreting hormones, including the incretins glucagon-like peptide 1 (GLP-1) and gastric inhibitory protein (GIP), which regulate multiple physiological processes. Hormone release is controlled through metabolite-sensing proteins. Low expression, interspecies differences, existence of EEC subtypes have posed challenges study these sensors. We describe differentiation stomach EECs complement existing...
The intestinal mucosal barrier contains microbial organisms within the lumen while preserving ability to absorb nutrients. Dietary, microbial, and other exposures shaped human evolution continue impact disease susceptibility. Here, we established engineered models of small intestine colon composed a multilineage epithelium, mucus layer, accessible compartment autologous tissue-resident immune cells. epithelium has crypt- villus-like topological domains, with stem cells differentiating into...