A5044529811- Cancer Cells and Metastasis
- CAR-T cell therapy research
- Artificial Immune Systems Applications
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Digestive system and related health
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Immune Cell Function and Interaction
- Biomedical and Engineering Education
- Single-cell and spatial transcriptomics
- Liver physiology and pathology
Roche (Switzerland)
2021-2025
École Polytechnique Fédérale de Lausanne
2021-2022
Epithelial organoids are stem cell–derived tissues that approximate aspects of real organs, and thus they have potential as powerful tools in basic translational research. By definition, self-organize, but the structures formed often heterogeneous irreproducible, which limits their use lab clinic. We describe methodologies for spatially temporally controlling organoid formation, thereby rendering a stochastic process more deterministic. Bioengineered cell microenvironments used to specify...
The intimate relationship between the epithelium and immune system is crucial for maintaining tissue homeostasis, with perturbations therein linked to autoimmune disease cancer
Abstract Predicting the toxicity of cancer immunotherapies preclinically is challenging because models tumours and healthy organs do not typically fully recapitulate expression relevant human antigens. Here we show that patient-derived intestinal organoids tumouroids supplemented with immune cells can be used to study on-target off-tumour toxicities T-cell-engaging bispecific antibodies (TCBs), capture clinical predicted by conventional tissue-based as well inter-patient variabilities in TCB...
Human complex in vitro models (CIVMs) have demonstrated remarkable potential to study tissue development, physiology and disease at high-throughput. To effectively employ these miniaturized systems translational preclinical research, their in-depth benchmarking is pivotal. Histology has been the core of characterization for centuries foundation spatial phenotyping. However, standard histology workflows are inherently low-throughput centered on large pieces. This does not match high sample...
The intestinal mucosal barrier contains microbial organisms within the lumen while preserving ability to absorb nutrients. Dietary, microbial, and other exposures shaped human evolution continue impact disease susceptibility. Here, we established engineered models of small intestine colon composed a multilineage epithelium, mucus layer, accessible compartment autologous tissue-resident immune cells. epithelium has crypt- villus-like topological domains, with stem cells differentiating into...