A5061025737- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Renal and related cancers
- Acupuncture Treatment Research Studies
- RNA Research and Splicing
- Neonatal Respiratory Health Research
- Digestive system and related health
- Cancer Genomics and Diagnostics
- Polysaccharides and Plant Cell Walls
- Air Quality and Health Impacts
- Healthcare and Venom Research
- Congenital Diaphragmatic Hernia Studies
- Traditional Chinese Medicine Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Air Quality Monitoring and Forecasting
- Metabolomics and Mass Spectrometry Studies
- Cancer Research and Treatments
- Bioinformatics and Genomic Networks
- Cell Image Analysis Techniques
- Polysaccharides Composition and Applications
- Polymer composites and self-healing
- Recycling and Waste Management Techniques
- Toxic Organic Pollutants Impact
Shenyang Agricultural University
2021-2025
Hohai University
2024
University of Basel
2020-2023
Roche (Switzerland)
2021-2023
Institute of Molecular and Clinical Ophthalmology Basel
2019-2023
Shandong University
2020-2023
Changchun University of Chinese Medicine
2022-2023
Northeast Forestry University
2023
Shandong Academy of Agricultural Sciences
2023
Institute of Plant Protection
2023
Organs are composed of diverse cell types that traverse transient states during organogenesis. To interrogate this diversity human development, we generate a single-cell transcriptome atlas from multiple developing endodermal organs the respiratory and gastrointestinal tract. We illuminate states, transcription factors, organ-specific epithelial stem mesenchyme interactions across lineages. implement as high-dimensional search space to benchmark pluripotent (hPSC)-derived intestinal...
Abstract Organoids generated from human pluripotent stem cells provide experimental systems to study development and disease, but quantitative measurements across different spatial scales molecular modalities are lacking. In this study, we multiplexed protein maps over a retinal organoid time course primary adult tissue. We developed toolkit visualize progenitor neuron location, the arrangements of extracellular subcellular components global patterning in each addition, single-cell...
Lipids are essential to brain functions, yet they remain largely unexplored. Here we investigated the lipidome composition of prefrontal cortex gray matter in 396 cognitively healthy individuals with ages spanning 100 years, as well 67 adult diagnosed autism (ASD), schizophrenia (SZ), and Down syndrome (DS). Of 5024 detected lipids, 95% showed significant age-dependent concentration differences clustering into four temporal stages, resulting a gradual increase membrane fluidity ranging from...
Physiochemical properties of engineered nanoparticles (NPs) play a vital role in nano-bio interactions, which are critical for nanotoxicity and nanomedicine research. To understand the effects NP hydrophobicity on formation protein corona, we synthesized four gold NPs with continuous change ranging from -2.6 to 2.4. Hydrophobic adsorbed 2.1-fold proteins compared hydrophilic ones. Proteins small molecular weights (<50 kDa) negatively charge (PI < 7) constituted majority especially...
Abstract The functions of human brains highly depend on the precise temporal regulation gene expression, and brain transcriptome profile across lifespan has been observed. substantial alteration in neural disorders like autism also observed is thought to be important for pathology. While cell type composition known variable brains, it remains unclear how contributes pathological changes brains. Here, we applied a deconvolution procedure an age series RNA-seq dataset healthy samples, quantify...
Epithelial organoids derived from intestinal tissue, called enteroids, recapitulate many aspects of the organ in vitro and can be used for biological discovery, personalized medicine, drug development. Here, we interrogated cell signaling environment within developing human intestine to identify niche cues that may important epithelial development homeostasis. We identified an EGF family member, EPIREGULIN (EREG), which is robustly expressed crypt. Enteroids generated grown standard culture...
The spatiotemporal distribution of recent human adaptation is a long standing question. We developed new coalescent-based method that collectively assigned genome regions to modes neutrality or positive, negative, balancing selection. Most importantly, the selection times were estimated for all positive signals, which ranged over last half million years, penetrating emergence anatomically modern (AMH). These time estimates further supported by analyses sequences from three ancient AMHs and...
In recent years, superhydrophobic coatings with self-cleaning abilities have attracted considerable attention. this study, we introduced hydroxyl-terminated polydimethylsiloxane (OH−PDMS) into castor-oil-based waterborne polyurethanes and synthesized silicone-modified UV-curable (SCWPU). Further, identified the optimal amount of OH−PDMS to be added different amounts micro- nanoscale heptadecafluorodecyltrimethoxysilane-modified SiO2 particles (FAS−SiO2) prepare rough-surface SCWPU dense...
Lung-resident immune cells, spanning both innate and adaptive compartments, preserve the integrity of respiratory barrier, but become pathogenic if dysregulated1. Current in vitro organoid models aim to replicate interactions between alveolar epithelium cells have not yet incorporated lung-specific critical for tissue residency2. Here we address this shortcoming by describing human lung immuno-organoids (LIO) that contain an autologous tissue-resident lymphoid compartment, primarily composed...
The intestinal mucosal barrier contains microbial organisms within the lumen while preserving ability to absorb nutrients. Dietary, microbial, and other exposures shaped human evolution continue impact disease susceptibility. Here, we established engineered models of small intestine colon composed a multilineage epithelium, mucus layer, accessible compartment autologous tissue-resident immune cells. epithelium has crypt- villus-like topological domains, with stem cells differentiating into...
Organoids generated from human pluripotent stem cells (PSCs) provide experimental systems to study development and disease. However, we lack quantitative spatiotemporal descriptions of organoid that incorporate measurements across different molecular modalities. Here focus on the retina use a single-cell multimodal approach reconstruct retinal development. We establish an computational pipeline generate multiplexed spatial protein maps over time course primary adult retina, registering...
PM2.5 is a complex pollutant that pervasive threat to human health. The health risks and toxicity mechanisms of components must be identified alleviate the corresponding risks. In this study, reductionism approach based on model particles was used investigate contributions most harmful in its toxicity. Human liver kidney cells were as models. results showed Cr(VI) critical toxic component among other (Pb, As, benzo[a]pyrene) cells. PM2.5-Cr(VI) induced oxidative stress, which led...
Abstract Bumblebees are important pollinators in agricultural ecosystems, but their abundance is declining globally. There an urgent need to protect bumblebee health and pollination services. possess specialized gut microbiota with potential be used as probiotics help defend at‐risk populations. However, evidence for probiotic benefits on bumblebees lacking. Here, we evaluated how supplementation Lactobacillus melliventris isolated from affected the colony development of Bombus terrestris ....