A5090495175- Neonatal Respiratory Health Research
- Optical Coherence Tomography Applications
- Congenital Diaphragmatic Hernia Studies
- Renal and related cancers
- Systemic Sclerosis and Related Diseases
- Tissue Engineering and Regenerative Medicine
- Tracheal and airway disorders
- Angiogenesis and VEGF in Cancer
- Asthma and respiratory diseases
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Dementia and Cognitive Impairment Research
- Photoacoustic and Ultrasonic Imaging
- Toxoplasma gondii Research Studies
- Allergic Rhinitis and Sensitization
- Protease and Inhibitor Mechanisms
- Connexins and lens biology
- 3D Printing in Biomedical Research
- Wnt/β-catenin signaling in development and cancer
- Herpesvirus Infections and Treatments
- Digestive system and related health
- Treatment of Major Depression
- Cancer-related cognitive impairment studies
- Vagus Nerve Stimulation Research
- AI in cancer detection
Yale University
2022-2024
The University of Texas Health Science Center at Houston
2024
University of Michigan
2016-2023
Children's Hospital at Westmead
2023
North Greenville University
2022-2023
Michigan United
2023
Miami University
2019-2022
California Institute for Regenerative Medicine
2020
University of Pennsylvania
2020
Massachusetts General Hospital
2014-2019
The current study aimed to understand the developmental mechanisms regulating bud tip progenitor cells in human fetal lung, which are present during branching morphogenesis, and use this information induce a progenitor-like population from pluripotent stem (hPSCs) vitro. We identified cues that maintained isolated lung epithelial vitro induced three-dimensional hPSC-derived organoids with tip-like domains. Bud domains could be isolated, expanded, as nearly homogeneous population. Molecular...
Human pluripotent stem cell (hPSC) derived tissues often remain developmentally immature in vitro, and become more adult-like their structure, cellular diversity function following transplantation into immunocompromised mice. Previously we have demonstrated that hPSC-derived human lung organoids (HLOs) resembled fetal tissue vitro (<xref ref-type="bibr" rid="bib10">Dye et al., 2015</xref>). Here show HLOs required a bioartificial microporous poly(lactide-co-glycolide) (PLG)...
Many lung diseases result from a failure of efficient regeneration damaged alveolar epithelial cells (AECs) after injury. During regeneration, AEC2s proliferate to replace lost cells, which proliferation halts and some transdifferentiate into AEC1s restore normal structure function. Although the mechanisms underlying AEC2 have been studied, responsible for halting inducing transdifferentiation are poorly understood. To identify candidate signaling pathways transdifferentiation, we performed...
The inability to visualize airway smooth muscle (ASM) cells in vivo is a major obstacle understanding their role normal physiology and diseases. At present, there no imaging modality available assess ASM vivo. Confocal endomicroscopy lacks the penetration depth field of view, conventional optical coherence tomography (OCT) does not have sufficient contrast differentiate from surrounding tissues. We developed birefringence microscopy platform that leverages micro-organization tissue add...
The intestine is maintained by stem cells located at the base of crypts and distinguished expression LGR5. Genetically engineered mouse models have provided a wealth information about intestinal cells, whereas less known human owing to difficulty detecting isolating these cells. We established an organoid repository from patient-derived adenomas, adenocarcinomas normal colon, which we analyzed for variants in 71 colorectal cancer (CRC)-associated genes. Normal neoplastic colon tissue...
Lung carcinoma diagnosis on tissue biopsy can be challenging because of insufficient tumor and lack architectural information. Optical coherence tomography (OCT) is a high-resolution imaging modality that visualizes microarchitecture in volumes orders magnitude larger than biopsy. It has been proposed OCT could potentially replace biopsy.We aim to determine whether histology diagnosing lung carcinomas. We develop validate interpretation criteria for common primary carcinomas: adenocarcinoma,...
The human respiratory epithelium is derived from a progenitor cell in the distal buds of developing lung. These "bud tip progenitors" are regulated by reciprocal signaling with surrounding mesenchyme; however, mesenchymal heterogeneity and function lung poorly understood. We interrogated single-cell RNA sequencing data multiple specimens identified population present during development that highly enriched for expression WNT agonist RSPO2, we found adjacent bud progenitors RSPO2 receptor...
Using scRNA-seq and microscopy, we describe a cell that is enriched in the lower airways of developing human lung identified by unique coexpression SCGB3A2/SFTPB/CFTR . To functionally interrogate these cells, apply single-cell barcode-based lineage tracing method, called CellTagging, to track fate cells during airway organoid differentiation vitro. Lineage reveals have distinct potential from basal giving rise predominantly pulmonary neuroendocrine subset multiciliated distinguished high C6...
With recent advancements in personalized medicine, biopsies must contain sufficient tumor for histologic diagnosis and molecular testing. However, inadvertent biopsy of tumor-associated fibrosis compromises yield, resulting delayed diagnoses and/or repeat procedures when additional is needed. The ability to differentiate from intraprocedurally during could significantly increase yield. Polarization-sensitive optical coherence tomography (PS-OCT) an imaging modality that endoscope-...
Toxoplasmosis is a potentially fatal infection for immunocompromised people and the developing fetus. Current medicines toxoplasmosis have high rates of adverse effects that interfere with therapeutic prophylactic regimens. Endochin-like quinolones (ELQs) are potent inhibitors Toxoplasma gondii proliferation in vitro animal models acute latent infection. ELQ-316, particular, was found to be effective orally against mice highly selective T. cytochrome b over human .
Expansion of pulmonary neuroendocrine cells (PNECs) is a pathological feature many human lung diseases. Human PNECs are inherently difficult to study due their rarity (<1% total cells) and lack established protocols for isolation. We used induced pluripotent stem (iPSCs) generate (iPNECs), which express core PNEC markers, including ROBO receptors, secrete major neuropeptides, recapitulating known functions primary PNECs. Furthermore, we demonstrate that differentiation efficiency increased...
ABSTRACT Background and objective In vivo evaluation of the microstructural differences between asthmatic non‐asthmatic airways their functional consequences is relevant to understanding and, potentially, treating asthma. this study, we use endobronchial optical coherence tomography investigate how allergic with asthma differ from in baseline microstructure response allergen challenge. Methods A total 45 subjects completed including 20 allergic, mildly individuals, 22 controls 3 healthy...
Human lung organoids (HLOs) are enabling the study of human development and disease by modeling native organ tissue structure, cellular composition, organization. In this report, we demonstrate that HLOs derived from pluripotent stem cells cultured in alginate, a fully defined nonanimal product substrate, exhibit enhanced differentiation compared with commercially available Matrigel. More specifically, observed an earlier onset increase number multiciliated cells, along mucus producing...
Fibroblast growth factor (FGF) signaling is known to play an important role in lung organogenesis. However, we recently demonstrated that FGF10 fails induce branching human fetal lungs as observed mouse. Our previous RNA sequencing data exhibited increased FGF18 during the pseudoglandular stage of development, suggestive its importance morphogenesis. Whereas it has been previously reported critical alveologenesis, few studies have described implication branching, specifically human....
Simplified methods of acquisition and quantification would facilitate the use synaptic density imaging in multicenter longitudinal studies Alzheimer disease (AD). We validated a simplified tissue-to-reference ratio method using SUV ratios (SUVRs) for estimating with [
SUMMARY Mammalian respiratory system development is regulated by complex reciprocal signaling events that take place between epithelial cells and the surrounding mesenchymal cells; however, heterogeneity function in developing human lung poorly understood. We interrogated single cell RNA sequencing data from multiple specimens identified a population present during highly enriched for expression of WNT agonist R-SPONDIN2 ( RSPO2 ), we found adjacent bud tip progenitors are receptor LGR5 . By...