A5021823990- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Drug-Induced Hepatotoxicity and Protection
- Iron Metabolism and Disorders
- Ferroptosis and cancer prognosis
- Nephrotoxicity and Medicinal Plants
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Hemoglobinopathies and Related Disorders
- Trace Elements in Health
- Immune cells in cancer
- Metabolism, Diabetes, and Cancer
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Interference and Gene Delivery
- Drug Transport and Resistance Mechanisms
- Paraquat toxicity studies and treatments
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Inflammatory mediators and NSAID effects
- Circular RNAs in diseases
University of New Mexico
2018-2025
Guangxi University
2025
Nanfang Hospital
2011-2024
Southern Medical University
2011-2024
First Affiliated Hospital of Wannan Medical College
2022-2024
Jinan University
2023-2024
Nanjing University
2023-2024
Chinese People's Liberation Army
2024
Sichuan Agricultural University
2023
Nanjing Medical University
2018-2023
Oxidative stress is an important event under both physiological and pathological conditions. In this study, we demonstrate how to quantify oxidative by measuring total reactive oxygen species (ROS) using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining in colorectal cancer cell lines as example. This protocol describes detailed steps including preparation of DCFH-DA solution, incubation cells with measurement normalized intensity. a simple cost-effective way detect ROS cells. It...
Oxygen dynamics in the liver is a central signaling mediator controlling hepatic homeostasis, and dysregulation of cellular oxygen associated with injury. Moreover, transcription factor relaying changes levels, hypoxia-inducible (HIF), critical metabolism, sustained increase HIF can lead to spontaneous steatosis, inflammation, tumorigenesis. However, direct responses genetic networks regulated by HIFs are unclear. To help define signal-transduction pathway, an animal model overexpression was...
Anti-SARS-CoV-2 virus antibody levels in convalescent plasma (CP), which may be useful severe infections, have been rarely reported.A total of eight donors were considered for enrollment; two them excluded because ineligible routine check. Of the six remaining participants, five samples tested weakly positive by IgM ELISA. Meanwhile, high titers IgG observed samples. The patient treated with CP did not require mechanical ventilation 11 days after transfusion, and was then transferred to a...
Abstract Hypoxia-inducible factor (HIF), a key modulator of the transcriptional response to hypoxia, is increased in colon cancer. However, role HIF carcinogenesis vivo remains unclear. In this study, we found that intestinal epithelium-specific disruption von Hippel–Lindau tumor suppressor protein (VHL) resulted constitutive signaling, and expression augmented tumorigenesis Apcmin/+ model. Intestine-specific Vhl multiplicity progression from adenomas carcinomas. These effects were...
Brahma-related gene 1 (BRG1) and long non-coding RNAs (lncRNAs) play important roles in cellular processes. However, little is known regarding their thoracic aortic aneurysms. We investigated BRG1 expression aneurysms the of lncRNA HIF alpha-antisense RNA regulating proliferation apoptosis smooth muscle cells vitro. mRNA protein human media specimens were examined by quantitative real-time polymerase chain reaction, immunohistochemical staining western blot. was up-regulated lentiviral...
Significance Inflammation is a major risk factor for many cancers and the role of metabolic reprogramming in inflammatory progression cancer not clear. We used quantitative proteomic approach to identify mitochondrial proteins that are altered early intestinal inflammation. show iron dysregulation an event initiates dysfunction. Through analysis, we identified reductase, six-transmembrane epithelial antigen prostate 4 (STEAP4), as being highly elevated during Using epithelial-specific STEAP4...
Abstract Microfold cells (M-cells) are specialized of the intestine that sample luminal microbiota and dietary antigens to educate immune intestinal lymphoid follicles. The function M-cells in systemic inflammatory responses still unclear. Here we show epithelial non-canonical NFkB signaling mediated by NFkB-inducing kinase (NIK) is highly active follicles, required for M-cell maintenance. Intestinal NIK modulates differentiation elicits both local IL-17A IgA production. Importantly, mouse...
Abstract Transferrin receptor (TFRC) is the major mediator for iron entry into a cell. Under excessive conditions, TFRC expected to be reduced lower uptake and toxicity. However, mechanism whereby expression maintained at high levels in iron‐enriched cancer cells contribution of development are enigmatic. Here work shows induced by adenomatous polyposis coli (APC) gene loss‐driven β ‐catenin activation colorectal cancer, whereas TFRC‐mediated intratumoral accumulation potentiates signaling...
Significance Several distinct congenital disorders can lead to tissue-iron overload with anemia. Tissue-iron accumulation is the major cause of mortality in these patients. Intestinal hypoxia-inducible factor-2α (HIF2α) and its downstream target gene divalent metal transporter-1 (DMT1) are essential for iron absorption during times increased demand. However, role intestinal HIF2α/DMT1 signaling axis has not been assessed. We demonstrate that HIF2α DMT1 small intestine highly activated early...
The mTOR complex 1 (mTORC1) and endoplasmic reticulum (ER) stress pathways are critical regulators of intestinal inflammation colon cancer growth. Sestrins stress-inducible proteins, which suppress both mTORC1 ER stress; however, the role in physiology tumorigenesis has been elusive due to lack studies human tissues or appropriate animal models. In this study, we show that SESN2 expression is elevated ulcerative colitis patients but lost upon p53 inactivation during carcinogenesis. mouse...
Major depressive disorder (MDD) is a considerable public health concern, which affects patients worldwide. MDD associated with psychosocial impairment, poor quality of life, and significant disability, morbidity mortality. Stress major factor in depression, impairs the structural functional plasticity hippocampus. Previous studies have demonstrated that chronic unpredictable mild stress able to downregulate expression brain‑derived neurotrophic (BDNF) methyl‑CpG‑binding protein 2 (MeCP2),...
The intestine is maintained by stem cells located at the base of crypts and distinguished expression LGR5. Genetically engineered mouse models have provided a wealth information about intestinal cells, whereas less known human owing to difficulty detecting isolating these cells. We established an organoid repository from patient-derived adenomas, adenocarcinomas normal colon, which we analyzed for variants in 71 colorectal cancer (CRC)-associated genes. Normal neoplastic colon tissue...